Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3177 and EX28DRAFT_3178 are separated by 9 nucleotides EX28DRAFT_3178 and EX28DRAFT_3179 are separated by 100 nucleotides
EX28DRAFT_3177: EX28DRAFT_3177 - serine transporter, at 477,894 to 479,225
_3177
EX28DRAFT_3178: EX28DRAFT_3178 - Threonine dehydratase, at 479,235 to 480,224
_3178
EX28DRAFT_3179: EX28DRAFT_3179 - Transcriptional regulator, at 480,325 to 481,245
_3179
Position (kb) 479
480
481 Strain fitness (log2 ratio) -2
-1
0
1
2 at 478.277 kb on - strand, within EX28DRAFT_3177 at 478.410 kb on - strand, within EX28DRAFT_3177 at 478.410 kb on - strand, within EX28DRAFT_3177 at 478.758 kb on - strand, within EX28DRAFT_3177 at 478.856 kb on + strand, within EX28DRAFT_3177 at 478.915 kb on + strand, within EX28DRAFT_3177 at 478.915 kb on + strand, within EX28DRAFT_3177 at 478.916 kb on - strand, within EX28DRAFT_3177 at 478.946 kb on + strand, within EX28DRAFT_3177 at 479.032 kb on + strand, within EX28DRAFT_3177 at 479.172 kb on - strand at 479.225 kb on + strand at 479.225 kb on + strand at 479.226 kb on - strand at 479.226 kb on - strand at 479.312 kb on + strand at 479.313 kb on - strand at 479.326 kb on + strand at 479.327 kb on - strand at 479.327 kb on - strand at 479.612 kb on + strand, within EX28DRAFT_3178 at 479.612 kb on + strand, within EX28DRAFT_3178 at 479.612 kb on + strand, within EX28DRAFT_3178 at 479.613 kb on - strand, within EX28DRAFT_3178 at 479.706 kb on + strand, within EX28DRAFT_3178 at 479.784 kb on - strand, within EX28DRAFT_3178 at 479.888 kb on + strand, within EX28DRAFT_3178 at 479.888 kb on + strand, within EX28DRAFT_3178 at 480.028 kb on - strand, within EX28DRAFT_3178 at 480.216 kb on - strand at 480.224 kb on + strand at 480.281 kb on + strand at 480.543 kb on + strand, within EX28DRAFT_3179 at 480.545 kb on + strand, within EX28DRAFT_3179 at 480.545 kb on + strand, within EX28DRAFT_3179 at 480.546 kb on - strand, within EX28DRAFT_3179 at 480.546 kb on - strand, within EX28DRAFT_3179 at 480.609 kb on + strand, within EX28DRAFT_3179 at 480.626 kb on + strand, within EX28DRAFT_3179 at 480.627 kb on - strand, within EX28DRAFT_3179 at 480.627 kb on - strand, within EX28DRAFT_3179 at 480.854 kb on + strand, within EX28DRAFT_3179 at 480.854 kb on + strand, within EX28DRAFT_3179 at 480.855 kb on - strand, within EX28DRAFT_3179 at 480.919 kb on - strand, within EX28DRAFT_3179 at 480.919 kb on - strand, within EX28DRAFT_3179 at 481.077 kb on + strand, within EX28DRAFT_3179 at 481.104 kb on - strand, within EX28DRAFT_3179 at 481.188 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 478,277 - EX28DRAFT_3177 0.29 -1.5 478,410 - EX28DRAFT_3177 0.39 +0.2 478,410 - EX28DRAFT_3177 0.39 +0.0 478,758 - EX28DRAFT_3177 0.65 +0.1 478,856 + EX28DRAFT_3177 0.72 -2.1 478,915 + EX28DRAFT_3177 0.77 -0.5 478,915 + EX28DRAFT_3177 0.77 -1.1 478,916 - EX28DRAFT_3177 0.77 +1.8 478,946 + EX28DRAFT_3177 0.79 +0.7 479,032 + EX28DRAFT_3177 0.85 -0.0 479,172 - +0.0 479,225 + +0.1 479,225 + +1.1 479,226 - +0.3 479,226 - +1.1 479,312 + +0.4 479,313 - -2.7 479,326 + -0.5 479,327 - -0.4 479,327 - -0.1 479,612 + EX28DRAFT_3178 0.38 -0.7 479,612 + EX28DRAFT_3178 0.38 -0.7 479,612 + EX28DRAFT_3178 0.38 +2.1 479,613 - EX28DRAFT_3178 0.38 +0.6 479,706 + EX28DRAFT_3178 0.48 -2.5 479,784 - EX28DRAFT_3178 0.55 +1.0 479,888 + EX28DRAFT_3178 0.66 +0.2 479,888 + EX28DRAFT_3178 0.66 -0.4 480,028 - EX28DRAFT_3178 0.80 -1.8 480,216 - +0.3 480,224 + -0.6 480,281 + -0.5 480,543 + EX28DRAFT_3179 0.24 +0.2 480,545 + EX28DRAFT_3179 0.24 +2.0 480,545 + EX28DRAFT_3179 0.24 -2.1 480,546 - EX28DRAFT_3179 0.24 +0.2 480,546 - EX28DRAFT_3179 0.24 +2.1 480,609 + EX28DRAFT_3179 0.31 -0.4 480,626 + EX28DRAFT_3179 0.33 +0.1 480,627 - EX28DRAFT_3179 0.33 +0.9 480,627 - EX28DRAFT_3179 0.33 +0.2 480,854 + EX28DRAFT_3179 0.57 +1.1 480,854 + EX28DRAFT_3179 0.57 +0.2 480,855 - EX28DRAFT_3179 0.58 +0.2 480,919 - EX28DRAFT_3179 0.64 +1.5 480,919 - EX28DRAFT_3179 0.64 -1.5 481,077 + EX28DRAFT_3179 0.82 -0.8 481,104 - EX28DRAFT_3179 0.85 -0.0 481,188 + -0.7
Or see this region's nucleotide sequence