Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2989 and EX28DRAFT_2990 are separated by 21 nucleotides EX28DRAFT_2990 and EX28DRAFT_2991 overlap by 4 nucleotides EX28DRAFT_2991 and EX28DRAFT_2992 are separated by 3 nucleotides EX28DRAFT_2992 and EX28DRAFT_2993 overlap by 8 nucleotides
EX28DRAFT_2989: EX28DRAFT_2989 - pyridoxal phosphate enzyme, YggS family, at 300,812 to 301,513
_2989
EX28DRAFT_2990: EX28DRAFT_2990 - Predicted integral membrane protein, at 301,535 to 302,101
_2990
EX28DRAFT_2991: EX28DRAFT_2991 - TIGR00251 family protein, at 302,098 to 302,394
_2991
EX28DRAFT_2992: EX28DRAFT_2992 - non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family, at 302,398 to 302,991
_2992
EX28DRAFT_2993: EX28DRAFT_2993 - putative oxygen-independent coproporphyrinogen III oxidase, at 302,984 to 304,126
_2993
Position (kb) 302
303 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 301.401 kb on + strand, within EX28DRAFT_2989 at 301.432 kb on + strand, within EX28DRAFT_2989 at 301.462 kb on + strand at 301.511 kb on + strand at 301.512 kb on - strand at 301.514 kb on + strand at 301.515 kb on - strand at 301.579 kb on + strand at 301.801 kb on + strand, within EX28DRAFT_2990 at 302.021 kb on + strand, within EX28DRAFT_2990 at 302.022 kb on - strand, within EX28DRAFT_2990 at 302.068 kb on + strand at 302.143 kb on + strand, within EX28DRAFT_2991 at 302.144 kb on - strand, within EX28DRAFT_2991 at 302.144 kb on - strand, within EX28DRAFT_2991 at 302.144 kb on - strand, within EX28DRAFT_2991 at 302.144 kb on - strand, within EX28DRAFT_2991 at 302.145 kb on + strand, within EX28DRAFT_2991 at 302.199 kb on - strand, within EX28DRAFT_2991 at 302.325 kb on + strand, within EX28DRAFT_2991 at 302.326 kb on - strand, within EX28DRAFT_2991 at 302.326 kb on - strand, within EX28DRAFT_2991 at 302.326 kb on - strand, within EX28DRAFT_2991 at 302.326 kb on - strand, within EX28DRAFT_2991 at 302.393 kb on - strand at 302.393 kb on - strand at 302.460 kb on - strand, within EX28DRAFT_2992 at 302.460 kb on - strand, within EX28DRAFT_2992 at 302.460 kb on - strand, within EX28DRAFT_2992 at 302.724 kb on - strand, within EX28DRAFT_2992 at 302.769 kb on + strand, within EX28DRAFT_2992 at 302.966 kb on + strand at 303.013 kb on + strand at 303.014 kb on - strand at 303.014 kb on - strand at 303.141 kb on - strand, within EX28DRAFT_2993 at 303.463 kb on + strand, within EX28DRAFT_2993 at 303.667 kb on - strand, within EX28DRAFT_2993 at 303.667 kb on - strand, within EX28DRAFT_2993 at 303.720 kb on - strand, within EX28DRAFT_2993 at 303.744 kb on - strand, within EX28DRAFT_2993
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 301,401 + EX28DRAFT_2989 0.84 +1.3 301,432 + EX28DRAFT_2989 0.88 -0.7 301,462 + +0.5 301,511 + -2.7 301,512 - -0.0 301,514 + +0.6 301,515 - +0.3 301,579 + +0.4 301,801 + EX28DRAFT_2990 0.47 -0.3 302,021 + EX28DRAFT_2990 0.86 +0.2 302,022 - EX28DRAFT_2990 0.86 -0.2 302,068 + +1.1 302,143 + EX28DRAFT_2991 0.15 -1.1 302,144 - EX28DRAFT_2991 0.15 +0.6 302,144 - EX28DRAFT_2991 0.15 -1.1 302,144 - EX28DRAFT_2991 0.15 -1.5 302,144 - EX28DRAFT_2991 0.15 -0.9 302,145 + EX28DRAFT_2991 0.16 +0.5 302,199 - EX28DRAFT_2991 0.34 +0.1 302,325 + EX28DRAFT_2991 0.76 -0.3 302,326 - EX28DRAFT_2991 0.77 +0.4 302,326 - EX28DRAFT_2991 0.77 +0.7 302,326 - EX28DRAFT_2991 0.77 -0.2 302,326 - EX28DRAFT_2991 0.77 -1.1 302,393 - +0.2 302,393 - -0.5 302,460 - EX28DRAFT_2992 0.10 -1.3 302,460 - EX28DRAFT_2992 0.10 -1.0 302,460 - EX28DRAFT_2992 0.10 -0.3 302,724 - EX28DRAFT_2992 0.55 -0.4 302,769 + EX28DRAFT_2992 0.62 -2.1 302,966 + -0.8 303,013 + +0.2 303,014 - +0.5 303,014 - -0.1 303,141 - EX28DRAFT_2993 0.14 -3.2 303,463 + EX28DRAFT_2993 0.42 -0.2 303,667 - EX28DRAFT_2993 0.60 +0.6 303,667 - EX28DRAFT_2993 0.60 +0.0 303,720 - EX28DRAFT_2993 0.64 -0.0 303,744 - EX28DRAFT_2993 0.66 +0.4
Or see this region's nucleotide sequence