Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_2889

Experiment: M9 with sucrose and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_2887 and EX28DRAFT_2888 overlap by 10 nucleotidesEX28DRAFT_2888 and EX28DRAFT_2889 are separated by 75 nucleotidesEX28DRAFT_2889 and EX28DRAFT_2890 are separated by 208 nucleotidesEX28DRAFT_2890 and EX28DRAFT_2891 are separated by 44 nucleotidesEX28DRAFT_2891 and EX28DRAFT_2892 are separated by 88 nucleotides EX28DRAFT_2887: EX28DRAFT_2887 - cysteine desulfurase, sulfur acceptor subunit CsdE, at 192,678 to 193,124 _2887 EX28DRAFT_2888: EX28DRAFT_2888 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1, at 193,115 to 193,921 _2888 EX28DRAFT_2889: EX28DRAFT_2889 - Membrane-bound lytic murein transglycosylase, at 193,997 to 195,094 _2889 EX28DRAFT_2890: EX28DRAFT_2890 - tRNA, at 195,303 to 195,379 _2890 EX28DRAFT_2891: EX28DRAFT_2891 - tRNA, at 195,424 to 195,500 _2891 EX28DRAFT_2892: EX28DRAFT_2892 - N-acetylmuramoyl-L-alanine amidase, at 195,589 to 196,857 _2892 Position (kb) 193 194 195 196Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 193.551 kb on + strand, within EX28DRAFT_2888at 193.551 kb on + strand, within EX28DRAFT_2888at 193.552 kb on - strand, within EX28DRAFT_2888at 193.557 kb on + strand, within EX28DRAFT_2888at 193.558 kb on - strand, within EX28DRAFT_2888at 193.558 kb on - strand, within EX28DRAFT_2888at 193.574 kb on - strand, within EX28DRAFT_2888at 193.751 kb on - strand, within EX28DRAFT_2888at 193.865 kb on + strandat 193.866 kb on - strandat 193.922 kb on - strandat 193.922 kb on - strandat 193.935 kb on + strandat 193.935 kb on + strandat 193.935 kb on + strandat 193.936 kb on - strandat 193.936 kb on - strandat 193.968 kb on + strandat 193.969 kb on - strandat 193.969 kb on - strandat 194.064 kb on + strandat 194.064 kb on + strandat 194.065 kb on - strandat 194.098 kb on + strandat 194.106 kb on + strandat 194.106 kb on + strandat 194.107 kb on - strand, within EX28DRAFT_2889at 194.107 kb on - strand, within EX28DRAFT_2889at 194.194 kb on + strand, within EX28DRAFT_2889at 194.261 kb on + strand, within EX28DRAFT_2889at 194.496 kb on + strand, within EX28DRAFT_2889at 194.496 kb on + strand, within EX28DRAFT_2889at 194.497 kb on - strand, within EX28DRAFT_2889at 194.538 kb on + strand, within EX28DRAFT_2889at 194.589 kb on + strand, within EX28DRAFT_2889at 194.602 kb on - strand, within EX28DRAFT_2889at 194.668 kb on + strand, within EX28DRAFT_2889at 194.673 kb on + strand, within EX28DRAFT_2889at 194.674 kb on - strand, within EX28DRAFT_2889at 194.677 kb on + strand, within EX28DRAFT_2889at 194.678 kb on - strand, within EX28DRAFT_2889at 194.730 kb on + strand, within EX28DRAFT_2889at 194.730 kb on + strand, within EX28DRAFT_2889at 194.806 kb on + strand, within EX28DRAFT_2889at 194.806 kb on + strand, within EX28DRAFT_2889at 194.853 kb on + strand, within EX28DRAFT_2889at 194.853 kb on + strand, within EX28DRAFT_2889at 194.854 kb on - strand, within EX28DRAFT_2889at 194.854 kb on - strand, within EX28DRAFT_2889at 194.860 kb on + strand, within EX28DRAFT_2889at 194.874 kb on + strand, within EX28DRAFT_2889at 194.967 kb on - strand, within EX28DRAFT_2889at 195.000 kb on + strandat 195.001 kb on - strandat 195.001 kb on - strandat 195.001 kb on - strandat 195.073 kb on - strandat 195.095 kb on + strandat 195.095 kb on + strandat 195.096 kb on - strandat 195.096 kb on - strandat 195.096 kb on - strandat 195.098 kb on - strandat 195.098 kb on - strandat 195.098 kb on - strandat 195.098 kb on - strandat 195.098 kb on - strandat 195.098 kb on - strandat 195.248 kb on - strandat 195.339 kb on + strand, within EX28DRAFT_2890at 195.397 kb on - strandat 195.502 kb on + strandat 195.503 kb on - strandat 195.517 kb on + strandat 195.690 kb on + strandat 195.691 kb on - strandat 195.792 kb on + strand, within EX28DRAFT_2892at 195.885 kb on + strand, within EX28DRAFT_2892at 195.885 kb on + strand, within EX28DRAFT_2892at 195.885 kb on + strand, within EX28DRAFT_2892at 195.885 kb on + strand, within EX28DRAFT_2892at 195.886 kb on - strand, within EX28DRAFT_2892at 195.886 kb on - strand, within EX28DRAFT_2892at 195.886 kb on - strand, within EX28DRAFT_2892

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM
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193,551 + EX28DRAFT_2888 0.54 +0.9
193,551 + EX28DRAFT_2888 0.54 +0.6
193,552 - EX28DRAFT_2888 0.54 +0.4
193,557 + EX28DRAFT_2888 0.55 -0.0
193,558 - EX28DRAFT_2888 0.55 -0.8
193,558 - EX28DRAFT_2888 0.55 +1.2
193,574 - EX28DRAFT_2888 0.57 +0.1
193,751 - EX28DRAFT_2888 0.79 +1.4
193,865 + +0.7
193,866 - -1.0
193,922 - +0.2
193,922 - -0.3
193,935 + -0.8
193,935 + +2.2
193,935 + +1.6
193,936 - -0.1
193,936 - +1.5
193,968 + +0.3
193,969 - -0.1
193,969 - +0.1
194,064 + -0.1
194,064 + +0.3
194,065 - +0.8
194,098 + -0.9
194,106 + -1.1
194,106 + +1.0
194,107 - EX28DRAFT_2889 0.10 -0.1
194,107 - EX28DRAFT_2889 0.10 +0.2
194,194 + EX28DRAFT_2889 0.18 +0.5
194,261 + EX28DRAFT_2889 0.24 +0.7
194,496 + EX28DRAFT_2889 0.45 +0.3
194,496 + EX28DRAFT_2889 0.45 -1.9
194,497 - EX28DRAFT_2889 0.46 -0.5
194,538 + EX28DRAFT_2889 0.49 +0.2
194,589 + EX28DRAFT_2889 0.54 +0.0
194,602 - EX28DRAFT_2889 0.55 -0.6
194,668 + EX28DRAFT_2889 0.61 +0.2
194,673 + EX28DRAFT_2889 0.62 +1.1
194,674 - EX28DRAFT_2889 0.62 -0.1
194,677 + EX28DRAFT_2889 0.62 +1.4
194,678 - EX28DRAFT_2889 0.62 -0.6
194,730 + EX28DRAFT_2889 0.67 +0.6
194,730 + EX28DRAFT_2889 0.67 +0.3
194,806 + EX28DRAFT_2889 0.74 -0.1
194,806 + EX28DRAFT_2889 0.74 -1.2
194,853 + EX28DRAFT_2889 0.78 -0.0
194,853 + EX28DRAFT_2889 0.78 +1.4
194,854 - EX28DRAFT_2889 0.78 +0.4
194,854 - EX28DRAFT_2889 0.78 -0.3
194,860 + EX28DRAFT_2889 0.79 -1.8
194,874 + EX28DRAFT_2889 0.80 +0.9
194,967 - EX28DRAFT_2889 0.88 +1.0
195,000 + +0.3
195,001 - +0.5
195,001 - +1.0
195,001 - +0.2
195,073 - -0.1
195,095 + -0.0
195,095 + +0.9
195,096 - +0.5
195,096 - +0.6
195,096 - -0.4
195,098 - -2.8
195,098 - +1.0
195,098 - +0.8
195,098 - -1.9
195,098 - +0.5
195,098 - +0.4
195,248 - -0.7
195,339 + EX28DRAFT_2890 0.47 -0.5
195,397 - +1.1
195,502 + -0.5
195,503 - -0.6
195,517 + -0.0
195,690 + +0.3
195,691 - +0.8
195,792 + EX28DRAFT_2892 0.16 +0.5
195,885 + EX28DRAFT_2892 0.23 -0.9
195,885 + EX28DRAFT_2892 0.23 -0.1
195,885 + EX28DRAFT_2892 0.23 -0.2
195,885 + EX28DRAFT_2892 0.23 -0.6
195,886 - EX28DRAFT_2892 0.23 +0.5
195,886 - EX28DRAFT_2892 0.23 +0.8
195,886 - EX28DRAFT_2892 0.23 +0.5

Or see this region's nucleotide sequence