Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2807 and EX28DRAFT_2808 are separated by 86 nucleotides EX28DRAFT_2808 and EX28DRAFT_2809 overlap by 8 nucleotides EX28DRAFT_2809 and EX28DRAFT_2810 overlap by 4 nucleotides EX28DRAFT_2810 and EX28DRAFT_2811 overlap by 1 nucleotides EX28DRAFT_2811 and EX28DRAFT_2812 are separated by 9 nucleotides
EX28DRAFT_2807: EX28DRAFT_2807 - Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase, at 111,312 to 112,736
_2807
EX28DRAFT_2808: EX28DRAFT_2808 - hydrogenase maturation protease HycI, at 112,823 to 113,278
_2808
EX28DRAFT_2809: EX28DRAFT_2809 - Formate hydrogenlyase maturation protein HycH, at 113,271 to 113,681
_2809
EX28DRAFT_2810: EX28DRAFT_2810 - Ni,Fe-hydrogenase III small subunit, at 113,678 to 114,445
_2810
EX28DRAFT_2811: EX28DRAFT_2811 - Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I), at 114,445 to 114,987
_2811
EX28DRAFT_2812: EX28DRAFT_2812 - Ni,Fe-hydrogenase III large subunit, at 114,997 to 116,706
_2812
Position (kb) 113
114
115 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 112.696 kb on - strand at 112.697 kb on + strand at 112.697 kb on + strand at 112.697 kb on + strand at 112.698 kb on - strand at 112.698 kb on - strand at 112.698 kb on - strand at 112.789 kb on + strand at 112.873 kb on - strand, within EX28DRAFT_2808 at 112.877 kb on + strand, within EX28DRAFT_2808 at 112.877 kb on + strand, within EX28DRAFT_2808 at 112.877 kb on + strand, within EX28DRAFT_2808 at 112.917 kb on + strand, within EX28DRAFT_2808 at 112.918 kb on - strand, within EX28DRAFT_2808 at 112.918 kb on - strand, within EX28DRAFT_2808 at 112.918 kb on - strand, within EX28DRAFT_2808 at 112.918 kb on - strand, within EX28DRAFT_2808 at 112.992 kb on + strand, within EX28DRAFT_2808 at 112.993 kb on - strand, within EX28DRAFT_2808 at 112.993 kb on - strand, within EX28DRAFT_2808 at 112.993 kb on - strand, within EX28DRAFT_2808 at 113.005 kb on + strand, within EX28DRAFT_2808 at 113.024 kb on - strand, within EX28DRAFT_2808 at 113.299 kb on + strand at 113.590 kb on + strand, within EX28DRAFT_2809 at 113.590 kb on + strand, within EX28DRAFT_2809 at 113.593 kb on + strand, within EX28DRAFT_2809 at 113.594 kb on - strand, within EX28DRAFT_2809 at 113.594 kb on - strand, within EX28DRAFT_2809 at 113.640 kb on - strand, within EX28DRAFT_2809 at 113.646 kb on - strand at 113.705 kb on - strand at 113.705 kb on - strand at 114.024 kb on + strand, within EX28DRAFT_2810 at 114.331 kb on - strand, within EX28DRAFT_2810 at 114.331 kb on - strand, within EX28DRAFT_2810 at 114.463 kb on - strand at 114.701 kb on + strand, within EX28DRAFT_2811 at 114.702 kb on - strand, within EX28DRAFT_2811 at 114.702 kb on - strand, within EX28DRAFT_2811 at 114.777 kb on + strand, within EX28DRAFT_2811 at 114.778 kb on - strand, within EX28DRAFT_2811 at 114.981 kb on + strand at 114.982 kb on - strand at 114.982 kb on - strand at 114.982 kb on - strand at 114.999 kb on - strand at 115.049 kb on + strand at 115.113 kb on - strand at 115.173 kb on + strand, within EX28DRAFT_2812 at 115.174 kb on - strand, within EX28DRAFT_2812 at 115.174 kb on - strand, within EX28DRAFT_2812 at 115.175 kb on + strand, within EX28DRAFT_2812 at 115.175 kb on + strand, within EX28DRAFT_2812 at 115.175 kb on + strand, within EX28DRAFT_2812 at 115.176 kb on - strand, within EX28DRAFT_2812 at 115.199 kb on + strand, within EX28DRAFT_2812 at 115.317 kb on - strand, within EX28DRAFT_2812
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 112,696 - +1.2 112,697 + -0.2 112,697 + -0.2 112,697 + +0.1 112,698 - -0.2 112,698 - +0.9 112,698 - -0.8 112,789 + +0.4 112,873 - EX28DRAFT_2808 0.11 +0.6 112,877 + EX28DRAFT_2808 0.12 -1.2 112,877 + EX28DRAFT_2808 0.12 -0.4 112,877 + EX28DRAFT_2808 0.12 +0.6 112,917 + EX28DRAFT_2808 0.21 +0.1 112,918 - EX28DRAFT_2808 0.21 +0.4 112,918 - EX28DRAFT_2808 0.21 -0.3 112,918 - EX28DRAFT_2808 0.21 +2.7 112,918 - EX28DRAFT_2808 0.21 -0.2 112,992 + EX28DRAFT_2808 0.37 +0.1 112,993 - EX28DRAFT_2808 0.37 +0.2 112,993 - EX28DRAFT_2808 0.37 -0.5 112,993 - EX28DRAFT_2808 0.37 +0.6 113,005 + EX28DRAFT_2808 0.40 -0.0 113,024 - EX28DRAFT_2808 0.44 +1.0 113,299 + -0.1 113,590 + EX28DRAFT_2809 0.78 +0.1 113,590 + EX28DRAFT_2809 0.78 +0.3 113,593 + EX28DRAFT_2809 0.78 -0.5 113,594 - EX28DRAFT_2809 0.79 +0.2 113,594 - EX28DRAFT_2809 0.79 +0.6 113,640 - EX28DRAFT_2809 0.90 -0.2 113,646 - -4.5 113,705 - +0.5 113,705 - -0.4 114,024 + EX28DRAFT_2810 0.45 +0.4 114,331 - EX28DRAFT_2810 0.85 +1.2 114,331 - EX28DRAFT_2810 0.85 +0.3 114,463 - -0.0 114,701 + EX28DRAFT_2811 0.47 -1.9 114,702 - EX28DRAFT_2811 0.47 +1.1 114,702 - EX28DRAFT_2811 0.47 -1.1 114,777 + EX28DRAFT_2811 0.61 +1.3 114,778 - EX28DRAFT_2811 0.61 -0.8 114,981 + +0.1 114,982 - -0.6 114,982 - +0.1 114,982 - +0.1 114,999 - -0.9 115,049 + +0.3 115,113 - +0.8 115,173 + EX28DRAFT_2812 0.10 -0.1 115,174 - EX28DRAFT_2812 0.10 -0.3 115,174 - EX28DRAFT_2812 0.10 -3.4 115,175 + EX28DRAFT_2812 0.10 +1.8 115,175 + EX28DRAFT_2812 0.10 +0.4 115,175 + EX28DRAFT_2812 0.10 +1.0 115,176 - EX28DRAFT_2812 0.10 +0.6 115,199 + EX28DRAFT_2812 0.12 +0.1 115,317 - EX28DRAFT_2812 0.19 -0.4
Or see this region's nucleotide sequence