Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2779 and EX28DRAFT_2780 are separated by 29 nucleotides EX28DRAFT_2780 and EX28DRAFT_2781 are separated by 4 nucleotides EX28DRAFT_2781 and EX28DRAFT_2782 are separated by 342 nucleotides EX28DRAFT_2782 and EX28DRAFT_2783 are separated by 241 nucleotides EX28DRAFT_2783 and EX28DRAFT_2784 are separated by 131 nucleotides EX28DRAFT_2784 and EX28DRAFT_2785 are separated by 69 nucleotides
EX28DRAFT_2779: EX28DRAFT_2779 - tRNA, at 84,872 to 84,948
_2779
EX28DRAFT_2780: EX28DRAFT_2780 - tRNA, at 84,978 to 85,054
_2780
EX28DRAFT_2781: EX28DRAFT_2781 - tRNA, at 85,059 to 85,151
_2781
EX28DRAFT_2782: EX28DRAFT_2782 - carbon storage regulator, CsrA, at 85,494 to 85,679
_2782
EX28DRAFT_2783: EX28DRAFT_2783 - alanyl-tRNA synthetase (EC 6.1.1.7), at 85,921 to 88,548
_2783
EX28DRAFT_2784: EX28DRAFT_2784 - Uncharacterized protein conserved in bacteria, at 88,680 to 89,180
_2784
EX28DRAFT_2785: EX28DRAFT_2785 - protein RecA, at 89,250 to 90,308
_2785
Position (kb) 85
86
87
88
89 Strain fitness (log2 ratio) -2
-1
0
1
2 at 85.374 kb on + strand at 85.739 kb on + strand at 85.740 kb on - strand at 85.740 kb on - strand at 85.741 kb on + strand at 85.742 kb on - strand at 85.773 kb on - strand at 85.773 kb on - strand at 85.779 kb on + strand at 85.812 kb on - strand at 85.827 kb on - strand at 88.703 kb on + strand at 88.704 kb on - strand at 88.712 kb on + strand at 88.713 kb on - strand at 88.735 kb on + strand, within EX28DRAFT_2784 at 88.796 kb on - strand, within EX28DRAFT_2784 at 88.796 kb on - strand, within EX28DRAFT_2784 at 88.799 kb on + strand, within EX28DRAFT_2784 at 88.799 kb on + strand, within EX28DRAFT_2784 at 88.800 kb on - strand, within EX28DRAFT_2784 at 88.991 kb on - strand, within EX28DRAFT_2784 at 89.014 kb on + strand, within EX28DRAFT_2784 at 89.015 kb on - strand, within EX28DRAFT_2784 at 89.015 kb on - strand, within EX28DRAFT_2784 at 89.139 kb on + strand at 89.149 kb on + strand at 89.181 kb on - strand at 89.265 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 85,374 + +1.4 85,739 + -2.4 85,740 - -0.3 85,740 - -0.3 85,741 + -0.1 85,742 - +0.1 85,773 - -0.6 85,773 - +0.1 85,779 + -2.2 85,812 - -0.5 85,827 - -0.9 88,703 + -0.1 88,704 - -1.6 88,712 + +0.7 88,713 - -0.1 88,735 + EX28DRAFT_2784 0.11 +2.6 88,796 - EX28DRAFT_2784 0.23 +0.0 88,796 - EX28DRAFT_2784 0.23 +1.2 88,799 + EX28DRAFT_2784 0.24 -0.0 88,799 + EX28DRAFT_2784 0.24 +0.4 88,800 - EX28DRAFT_2784 0.24 +0.8 88,991 - EX28DRAFT_2784 0.62 +0.2 89,014 + EX28DRAFT_2784 0.67 -0.2 89,015 - EX28DRAFT_2784 0.67 -0.1 89,015 - EX28DRAFT_2784 0.67 -0.5 89,139 + +0.3 89,149 + +0.3 89,181 - -0.4 89,265 - +0.4
Or see this region's nucleotide sequence