Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2062 and EX28DRAFT_2063 are separated by 38 nucleotides EX28DRAFT_2063 and EX28DRAFT_2064 are separated by 10 nucleotides
EX28DRAFT_2062: EX28DRAFT_2062 - Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase], at 2,084,914 to 2,086,632
_2062
EX28DRAFT_2063: EX28DRAFT_2063 - L-threonine aldolase (EC 4.1.2.5), at 2,086,671 to 2,087,672
_2063
EX28DRAFT_2064: EX28DRAFT_2064 - Predicted nucleoside-diphosphate-sugar epimerases, at 2,087,683 to 2,089,119
_2064
Position (kb)
2086
2087
2088 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2085.790 kb on - strand, within EX28DRAFT_2062 at 2085.849 kb on + strand, within EX28DRAFT_2062 at 2085.855 kb on + strand, within EX28DRAFT_2062 at 2085.856 kb on - strand, within EX28DRAFT_2062 at 2086.042 kb on - strand, within EX28DRAFT_2062 at 2086.042 kb on - strand, within EX28DRAFT_2062 at 2086.042 kb on - strand, within EX28DRAFT_2062 at 2086.081 kb on + strand, within EX28DRAFT_2062 at 2086.082 kb on - strand, within EX28DRAFT_2062 at 2086.551 kb on + strand at 2086.558 kb on + strand at 2086.558 kb on + strand at 2086.558 kb on + strand at 2086.558 kb on + strand at 2086.558 kb on + strand at 2086.559 kb on - strand at 2086.559 kb on - strand at 2086.559 kb on - strand at 2086.559 kb on - strand at 2086.559 kb on - strand at 2086.561 kb on - strand at 2086.561 kb on - strand at 2086.561 kb on - strand at 2086.659 kb on + strand at 2086.896 kb on + strand, within EX28DRAFT_2063 at 2086.896 kb on + strand, within EX28DRAFT_2063 at 2086.923 kb on + strand, within EX28DRAFT_2063 at 2086.923 kb on + strand, within EX28DRAFT_2063 at 2086.923 kb on + strand, within EX28DRAFT_2063 at 2086.923 kb on + strand, within EX28DRAFT_2063 at 2086.923 kb on + strand, within EX28DRAFT_2063 at 2086.924 kb on - strand, within EX28DRAFT_2063 at 2086.924 kb on - strand, within EX28DRAFT_2063 at 2086.924 kb on - strand, within EX28DRAFT_2063 at 2086.924 kb on - strand, within EX28DRAFT_2063 at 2086.924 kb on - strand, within EX28DRAFT_2063 at 2086.924 kb on - strand, within EX28DRAFT_2063 at 2087.112 kb on + strand, within EX28DRAFT_2063 at 2087.112 kb on + strand, within EX28DRAFT_2063 at 2087.177 kb on + strand, within EX28DRAFT_2063 at 2087.200 kb on - strand, within EX28DRAFT_2063 at 2087.307 kb on + strand, within EX28DRAFT_2063 at 2087.307 kb on + strand, within EX28DRAFT_2063 at 2087.366 kb on + strand, within EX28DRAFT_2063 at 2087.389 kb on - strand, within EX28DRAFT_2063 at 2087.601 kb on - strand at 2087.601 kb on - strand at 2087.719 kb on + strand at 2087.720 kb on - strand at 2087.722 kb on - strand at 2087.896 kb on + strand, within EX28DRAFT_2064 at 2087.897 kb on - strand, within EX28DRAFT_2064 at 2087.932 kb on - strand, within EX28DRAFT_2064 at 2088.250 kb on + strand, within EX28DRAFT_2064 at 2088.250 kb on + strand, within EX28DRAFT_2064 at 2088.326 kb on - strand, within EX28DRAFT_2064
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,085,790 - EX28DRAFT_2062 0.51 -0.1 2,085,849 + EX28DRAFT_2062 0.54 +1.0 2,085,855 + EX28DRAFT_2062 0.55 -2.5 2,085,856 - EX28DRAFT_2062 0.55 +0.2 2,086,042 - EX28DRAFT_2062 0.66 -0.9 2,086,042 - EX28DRAFT_2062 0.66 -0.9 2,086,042 - EX28DRAFT_2062 0.66 -2.3 2,086,081 + EX28DRAFT_2062 0.68 +0.1 2,086,082 - EX28DRAFT_2062 0.68 +1.3 2,086,551 + +0.7 2,086,558 + +0.1 2,086,558 + -1.2 2,086,558 + +0.3 2,086,558 + -2.9 2,086,558 + -0.0 2,086,559 - +0.4 2,086,559 - +1.1 2,086,559 - +0.1 2,086,559 - -0.3 2,086,559 - -0.7 2,086,561 - +2.4 2,086,561 - +0.9 2,086,561 - +0.0 2,086,659 + -3.4 2,086,896 + EX28DRAFT_2063 0.22 -1.3 2,086,896 + EX28DRAFT_2063 0.22 +0.2 2,086,923 + EX28DRAFT_2063 0.25 +0.1 2,086,923 + EX28DRAFT_2063 0.25 -0.8 2,086,923 + EX28DRAFT_2063 0.25 -0.3 2,086,923 + EX28DRAFT_2063 0.25 +1.4 2,086,923 + EX28DRAFT_2063 0.25 +0.1 2,086,924 - EX28DRAFT_2063 0.25 -0.1 2,086,924 - EX28DRAFT_2063 0.25 -1.5 2,086,924 - EX28DRAFT_2063 0.25 -1.5 2,086,924 - EX28DRAFT_2063 0.25 +0.7 2,086,924 - EX28DRAFT_2063 0.25 +3.1 2,086,924 - EX28DRAFT_2063 0.25 -0.6 2,087,112 + EX28DRAFT_2063 0.44 -0.0 2,087,112 + EX28DRAFT_2063 0.44 +0.3 2,087,177 + EX28DRAFT_2063 0.50 -0.7 2,087,200 - EX28DRAFT_2063 0.53 +0.6 2,087,307 + EX28DRAFT_2063 0.63 +0.0 2,087,307 + EX28DRAFT_2063 0.63 +1.1 2,087,366 + EX28DRAFT_2063 0.69 +0.1 2,087,389 - EX28DRAFT_2063 0.72 +0.5 2,087,601 - -0.8 2,087,601 - -0.5 2,087,719 + -0.5 2,087,720 - +0.0 2,087,722 - -0.7 2,087,896 + EX28DRAFT_2064 0.15 +0.1 2,087,897 - EX28DRAFT_2064 0.15 +0.1 2,087,932 - EX28DRAFT_2064 0.17 -0.3 2,088,250 + EX28DRAFT_2064 0.39 -0.3 2,088,250 + EX28DRAFT_2064 0.39 -0.1 2,088,326 - EX28DRAFT_2064 0.45 -0.2
Or see this region's nucleotide sequence