Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1976 and EX28DRAFT_1977 are separated by 60 nucleotides EX28DRAFT_1977 and EX28DRAFT_1978 are separated by 35 nucleotides EX28DRAFT_1978 and EX28DRAFT_1979 are separated by 173 nucleotides EX28DRAFT_1979 and EX28DRAFT_1980 are separated by 80 nucleotides
EX28DRAFT_1976: EX28DRAFT_1976 - Predicted SAM-dependent methyltransferases, at 1,977,698 to 1,978,888
_1976
EX28DRAFT_1977: EX28DRAFT_1977 - hemimethylated DNA binding domain, at 1,978,949 to 1,979,266
_1977
EX28DRAFT_1978: EX28DRAFT_1978 - Predicted CoA-binding protein, at 1,979,302 to 1,979,715
_1978
EX28DRAFT_1979: EX28DRAFT_1979 - Uncharacterized protein conserved in bacteria, at 1,979,889 to 1,980,551
_1979
EX28DRAFT_1980: EX28DRAFT_1980 - methylglyoxal synthase, at 1,980,632 to 1,981,090
_1980
Position (kb)
1979
1980 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1978.310 kb on + strand, within EX28DRAFT_1976 at 1978.311 kb on - strand, within EX28DRAFT_1976 at 1978.349 kb on + strand, within EX28DRAFT_1976 at 1978.386 kb on - strand, within EX28DRAFT_1976 at 1978.435 kb on - strand, within EX28DRAFT_1976 at 1978.435 kb on - strand, within EX28DRAFT_1976 at 1978.552 kb on + strand, within EX28DRAFT_1976 at 1978.552 kb on + strand, within EX28DRAFT_1976 at 1978.552 kb on + strand, within EX28DRAFT_1976 at 1978.552 kb on + strand, within EX28DRAFT_1976 at 1978.552 kb on + strand, within EX28DRAFT_1976 at 1978.553 kb on - strand, within EX28DRAFT_1976 at 1978.556 kb on + strand, within EX28DRAFT_1976 at 1978.557 kb on - strand, within EX28DRAFT_1976 at 1978.652 kb on - strand, within EX28DRAFT_1976 at 1978.652 kb on - strand, within EX28DRAFT_1976 at 1978.652 kb on - strand, within EX28DRAFT_1976 at 1978.657 kb on + strand, within EX28DRAFT_1976 at 1978.740 kb on + strand, within EX28DRAFT_1976 at 1978.740 kb on + strand, within EX28DRAFT_1976 at 1978.770 kb on + strand at 1978.770 kb on + strand at 1978.770 kb on + strand at 1978.770 kb on + strand at 1978.771 kb on - strand at 1978.796 kb on - strand at 1978.797 kb on + strand at 1978.797 kb on + strand at 1978.797 kb on + strand at 1978.886 kb on + strand at 1978.886 kb on + strand at 1978.886 kb on + strand at 1978.903 kb on + strand at 1978.916 kb on + strand at 1978.916 kb on + strand at 1978.917 kb on - strand at 1978.923 kb on + strand at 1978.923 kb on + strand at 1978.923 kb on + strand at 1978.923 kb on + strand at 1978.924 kb on - strand at 1978.928 kb on - strand at 1979.147 kb on + strand, within EX28DRAFT_1977 at 1979.148 kb on - strand, within EX28DRAFT_1977 at 1979.265 kb on - strand at 1979.265 kb on - strand at 1979.534 kb on + strand, within EX28DRAFT_1978 at 1979.612 kb on + strand, within EX28DRAFT_1978 at 1979.700 kb on + strand at 1979.778 kb on + strand at 1979.807 kb on + strand at 1979.807 kb on + strand at 1979.808 kb on - strand at 1979.808 kb on - strand at 1979.808 kb on - strand at 1979.808 kb on - strand at 1979.813 kb on + strand at 1979.813 kb on + strand at 1979.814 kb on - strand at 1979.814 kb on - strand at 1979.814 kb on - strand at 1979.814 kb on - strand at 1979.815 kb on + strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.816 kb on - strand at 1979.889 kb on - strand at 1979.986 kb on + strand, within EX28DRAFT_1979 at 1979.987 kb on - strand, within EX28DRAFT_1979 at 1980.117 kb on + strand, within EX28DRAFT_1979 at 1980.117 kb on + strand, within EX28DRAFT_1979 at 1980.117 kb on + strand, within EX28DRAFT_1979 at 1980.118 kb on - strand, within EX28DRAFT_1979 at 1980.118 kb on - strand, within EX28DRAFT_1979 at 1980.118 kb on - strand, within EX28DRAFT_1979 at 1980.118 kb on - strand, within EX28DRAFT_1979 at 1980.146 kb on + strand, within EX28DRAFT_1979 at 1980.282 kb on - strand, within EX28DRAFT_1979 at 1980.339 kb on + strand, within EX28DRAFT_1979 at 1980.339 kb on + strand, within EX28DRAFT_1979 at 1980.340 kb on - strand, within EX28DRAFT_1979 at 1980.549 kb on + strand at 1980.626 kb on - strand at 1980.626 kb on - strand at 1980.631 kb on + strand at 1980.697 kb on + strand, within EX28DRAFT_1980 at 1980.698 kb on - strand, within EX28DRAFT_1980 at 1980.713 kb on - strand, within EX28DRAFT_1980 at 1980.713 kb on - strand, within EX28DRAFT_1980 at 1980.713 kb on - strand, within EX28DRAFT_1980 at 1980.713 kb on - strand, within EX28DRAFT_1980
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,978,310 + EX28DRAFT_1976 0.51 +0.6 1,978,311 - EX28DRAFT_1976 0.51 +0.5 1,978,349 + EX28DRAFT_1976 0.55 +0.5 1,978,386 - EX28DRAFT_1976 0.58 +1.4 1,978,435 - EX28DRAFT_1976 0.62 -0.3 1,978,435 - EX28DRAFT_1976 0.62 +0.4 1,978,552 + EX28DRAFT_1976 0.72 +0.4 1,978,552 + EX28DRAFT_1976 0.72 -0.5 1,978,552 + EX28DRAFT_1976 0.72 +1.2 1,978,552 + EX28DRAFT_1976 0.72 -1.5 1,978,552 + EX28DRAFT_1976 0.72 -0.5 1,978,553 - EX28DRAFT_1976 0.72 -1.7 1,978,556 + EX28DRAFT_1976 0.72 -0.1 1,978,557 - EX28DRAFT_1976 0.72 +0.5 1,978,652 - EX28DRAFT_1976 0.80 +2.0 1,978,652 - EX28DRAFT_1976 0.80 -2.0 1,978,652 - EX28DRAFT_1976 0.80 -0.7 1,978,657 + EX28DRAFT_1976 0.81 -1.0 1,978,740 + EX28DRAFT_1976 0.87 +0.8 1,978,740 + EX28DRAFT_1976 0.87 -0.9 1,978,770 + +0.5 1,978,770 + -0.3 1,978,770 + -1.2 1,978,770 + +1.0 1,978,771 - -0.0 1,978,796 - -0.1 1,978,797 + +1.4 1,978,797 + -0.6 1,978,797 + -1.0 1,978,886 + +1.9 1,978,886 + -0.4 1,978,886 + -0.1 1,978,903 + +0.8 1,978,916 + -0.2 1,978,916 + -0.1 1,978,917 - -4.1 1,978,923 + -2.3 1,978,923 + -0.0 1,978,923 + -3.3 1,978,923 + +1.0 1,978,924 - -2.3 1,978,928 - -0.0 1,979,147 + EX28DRAFT_1977 0.62 +0.6 1,979,148 - EX28DRAFT_1977 0.63 -0.6 1,979,265 - +0.3 1,979,265 - -0.4 1,979,534 + EX28DRAFT_1978 0.56 +1.4 1,979,612 + EX28DRAFT_1978 0.75 -2.0 1,979,700 + -0.3 1,979,778 + +0.3 1,979,807 + +2.6 1,979,807 + +0.5 1,979,808 - +1.2 1,979,808 - +0.6 1,979,808 - +1.5 1,979,808 - +0.3 1,979,813 + -0.1 1,979,813 + -0.3 1,979,814 - +0.1 1,979,814 - -1.3 1,979,814 - -0.8 1,979,814 - -0.2 1,979,815 + +0.8 1,979,816 - -1.4 1,979,816 - +2.3 1,979,816 - +0.5 1,979,816 - -1.4 1,979,816 - -0.1 1,979,816 - +0.4 1,979,889 - +0.2 1,979,986 + EX28DRAFT_1979 0.15 +0.0 1,979,987 - EX28DRAFT_1979 0.15 -0.2 1,980,117 + EX28DRAFT_1979 0.34 -1.3 1,980,117 + EX28DRAFT_1979 0.34 -0.8 1,980,117 + EX28DRAFT_1979 0.34 +1.8 1,980,118 - EX28DRAFT_1979 0.35 +0.8 1,980,118 - EX28DRAFT_1979 0.35 +0.7 1,980,118 - EX28DRAFT_1979 0.35 -0.2 1,980,118 - EX28DRAFT_1979 0.35 -0.2 1,980,146 + EX28DRAFT_1979 0.39 -0.2 1,980,282 - EX28DRAFT_1979 0.59 -1.3 1,980,339 + EX28DRAFT_1979 0.68 +0.8 1,980,339 + EX28DRAFT_1979 0.68 -0.1 1,980,340 - EX28DRAFT_1979 0.68 -2.8 1,980,549 + +0.1 1,980,626 - -0.3 1,980,626 - +0.0 1,980,631 + -2.8 1,980,697 + EX28DRAFT_1980 0.14 -0.1 1,980,698 - EX28DRAFT_1980 0.14 +0.5 1,980,713 - EX28DRAFT_1980 0.18 -0.4 1,980,713 - EX28DRAFT_1980 0.18 -0.1 1,980,713 - EX28DRAFT_1980 0.18 -0.6 1,980,713 - EX28DRAFT_1980 0.18 +0.2
Or see this region's nucleotide sequence