Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1955 and EX28DRAFT_1956 overlap by 4 nucleotides EX28DRAFT_1956 and EX28DRAFT_1957 are separated by 9 nucleotides
EX28DRAFT_1955: EX28DRAFT_1955 - hypothetical protein, at 1,959,442 to 1,960,599
_1955
EX28DRAFT_1956: EX28DRAFT_1956 - hypothetical protein, at 1,960,596 to 1,960,718
_1956
EX28DRAFT_1957: EX28DRAFT_1957 - Predicted membrane protein involved in D-alanine export, at 1,960,728 to 1,961,846
_1957
Position (kb)
1960
1961 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1959.645 kb on - strand, within EX28DRAFT_1955 at 1959.706 kb on + strand, within EX28DRAFT_1955 at 1959.706 kb on + strand, within EX28DRAFT_1955 at 1959.707 kb on - strand, within EX28DRAFT_1955 at 1959.713 kb on + strand, within EX28DRAFT_1955 at 1959.713 kb on + strand, within EX28DRAFT_1955 at 1959.715 kb on + strand, within EX28DRAFT_1955 at 1959.716 kb on - strand, within EX28DRAFT_1955 at 1959.716 kb on - strand, within EX28DRAFT_1955 at 1959.716 kb on - strand, within EX28DRAFT_1955 at 1959.717 kb on + strand, within EX28DRAFT_1955 at 1959.895 kb on + strand, within EX28DRAFT_1955 at 1959.895 kb on + strand, within EX28DRAFT_1955 at 1959.895 kb on + strand, within EX28DRAFT_1955 at 1959.896 kb on - strand, within EX28DRAFT_1955 at 1959.967 kb on - strand, within EX28DRAFT_1955 at 1959.971 kb on - strand, within EX28DRAFT_1955 at 1959.975 kb on - strand, within EX28DRAFT_1955 at 1959.982 kb on - strand, within EX28DRAFT_1955 at 1959.987 kb on + strand, within EX28DRAFT_1955 at 1959.990 kb on + strand, within EX28DRAFT_1955 at 1960.418 kb on - strand, within EX28DRAFT_1955 at 1960.418 kb on - strand, within EX28DRAFT_1955 at 1960.422 kb on + strand, within EX28DRAFT_1955 at 1960.490 kb on + strand at 1960.490 kb on + strand at 1960.491 kb on - strand at 1960.494 kb on + strand at 1960.495 kb on - strand at 1960.495 kb on - strand at 1960.495 kb on - strand at 1960.498 kb on + strand at 1960.498 kb on + strand at 1960.499 kb on - strand at 1960.566 kb on + strand at 1960.686 kb on - strand, within EX28DRAFT_1956 at 1960.731 kb on + strand at 1960.731 kb on + strand at 1960.732 kb on - strand at 1960.801 kb on + strand at 1960.861 kb on - strand, within EX28DRAFT_1957 at 1960.862 kb on + strand, within EX28DRAFT_1957 at 1960.862 kb on + strand, within EX28DRAFT_1957 at 1960.863 kb on - strand, within EX28DRAFT_1957 at 1960.863 kb on - strand, within EX28DRAFT_1957 at 1960.863 kb on - strand, within EX28DRAFT_1957 at 1960.864 kb on + strand, within EX28DRAFT_1957 at 1960.865 kb on - strand, within EX28DRAFT_1957 at 1960.879 kb on - strand, within EX28DRAFT_1957 at 1960.880 kb on + strand, within EX28DRAFT_1957 at 1960.911 kb on + strand, within EX28DRAFT_1957 at 1960.928 kb on - strand, within EX28DRAFT_1957 at 1960.928 kb on - strand, within EX28DRAFT_1957 at 1960.945 kb on + strand, within EX28DRAFT_1957 at 1960.999 kb on + strand, within EX28DRAFT_1957 at 1961.005 kb on + strand, within EX28DRAFT_1957 at 1961.133 kb on - strand, within EX28DRAFT_1957 at 1961.136 kb on - strand, within EX28DRAFT_1957 at 1961.364 kb on + strand, within EX28DRAFT_1957 at 1961.370 kb on + strand, within EX28DRAFT_1957 at 1961.370 kb on + strand, within EX28DRAFT_1957 at 1961.370 kb on + strand, within EX28DRAFT_1957 at 1961.371 kb on - strand, within EX28DRAFT_1957 at 1961.371 kb on - strand, within EX28DRAFT_1957 at 1961.397 kb on - strand, within EX28DRAFT_1957 at 1961.432 kb on + strand, within EX28DRAFT_1957 at 1961.544 kb on + strand, within EX28DRAFT_1957 at 1961.546 kb on + strand, within EX28DRAFT_1957 at 1961.600 kb on + strand, within EX28DRAFT_1957 at 1961.620 kb on + strand, within EX28DRAFT_1957 at 1961.658 kb on + strand, within EX28DRAFT_1957 at 1961.705 kb on + strand, within EX28DRAFT_1957 at 1961.705 kb on + strand, within EX28DRAFT_1957 at 1961.705 kb on + strand, within EX28DRAFT_1957
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,959,645 - EX28DRAFT_1955 0.18 +0.3 1,959,706 + EX28DRAFT_1955 0.23 +0.2 1,959,706 + EX28DRAFT_1955 0.23 -0.3 1,959,707 - EX28DRAFT_1955 0.23 -0.1 1,959,713 + EX28DRAFT_1955 0.23 -0.1 1,959,713 + EX28DRAFT_1955 0.23 -2.3 1,959,715 + EX28DRAFT_1955 0.24 +1.6 1,959,716 - EX28DRAFT_1955 0.24 -0.1 1,959,716 - EX28DRAFT_1955 0.24 +0.2 1,959,716 - EX28DRAFT_1955 0.24 -1.3 1,959,717 + EX28DRAFT_1955 0.24 +0.0 1,959,895 + EX28DRAFT_1955 0.39 -2.0 1,959,895 + EX28DRAFT_1955 0.39 -2.6 1,959,895 + EX28DRAFT_1955 0.39 +0.4 1,959,896 - EX28DRAFT_1955 0.39 -2.3 1,959,967 - EX28DRAFT_1955 0.45 -1.2 1,959,971 - EX28DRAFT_1955 0.46 +0.2 1,959,975 - EX28DRAFT_1955 0.46 -0.1 1,959,982 - EX28DRAFT_1955 0.47 +0.2 1,959,987 + EX28DRAFT_1955 0.47 +1.3 1,959,990 + EX28DRAFT_1955 0.47 +0.5 1,960,418 - EX28DRAFT_1955 0.84 +1.3 1,960,418 - EX28DRAFT_1955 0.84 +1.2 1,960,422 + EX28DRAFT_1955 0.85 +0.0 1,960,490 + +2.1 1,960,490 + +1.3 1,960,491 - +0.0 1,960,494 + -0.6 1,960,495 - -3.2 1,960,495 - -1.0 1,960,495 - -1.0 1,960,498 + +0.3 1,960,498 + -0.4 1,960,499 - -0.5 1,960,566 + +0.3 1,960,686 - EX28DRAFT_1956 0.73 -4.0 1,960,731 + -0.4 1,960,731 + -0.8 1,960,732 - +0.2 1,960,801 + +0.0 1,960,861 - EX28DRAFT_1957 0.12 +0.2 1,960,862 + EX28DRAFT_1957 0.12 -2.0 1,960,862 + EX28DRAFT_1957 0.12 +0.4 1,960,863 - EX28DRAFT_1957 0.12 -2.0 1,960,863 - EX28DRAFT_1957 0.12 -0.4 1,960,863 - EX28DRAFT_1957 0.12 -1.0 1,960,864 + EX28DRAFT_1957 0.12 +0.2 1,960,865 - EX28DRAFT_1957 0.12 -0.4 1,960,879 - EX28DRAFT_1957 0.13 -0.2 1,960,880 + EX28DRAFT_1957 0.14 -0.0 1,960,911 + EX28DRAFT_1957 0.16 -0.7 1,960,928 - EX28DRAFT_1957 0.18 -0.6 1,960,928 - EX28DRAFT_1957 0.18 +0.2 1,960,945 + EX28DRAFT_1957 0.19 +0.0 1,960,999 + EX28DRAFT_1957 0.24 -0.5 1,961,005 + EX28DRAFT_1957 0.25 -0.4 1,961,133 - EX28DRAFT_1957 0.36 -1.2 1,961,136 - EX28DRAFT_1957 0.36 +0.3 1,961,364 + EX28DRAFT_1957 0.57 -0.4 1,961,370 + EX28DRAFT_1957 0.57 -0.0 1,961,370 + EX28DRAFT_1957 0.57 +0.8 1,961,370 + EX28DRAFT_1957 0.57 -0.2 1,961,371 - EX28DRAFT_1957 0.57 -1.7 1,961,371 - EX28DRAFT_1957 0.57 -0.4 1,961,397 - EX28DRAFT_1957 0.60 +0.0 1,961,432 + EX28DRAFT_1957 0.63 -1.1 1,961,544 + EX28DRAFT_1957 0.73 -0.8 1,961,546 + EX28DRAFT_1957 0.73 +0.5 1,961,600 + EX28DRAFT_1957 0.78 -0.3 1,961,620 + EX28DRAFT_1957 0.80 -0.7 1,961,658 + EX28DRAFT_1957 0.83 -0.3 1,961,705 + EX28DRAFT_1957 0.87 -0.2 1,961,705 + EX28DRAFT_1957 0.87 +0.5 1,961,705 + EX28DRAFT_1957 0.87 -1.0
Or see this region's nucleotide sequence