Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1894 and EX28DRAFT_1895 are separated by 21 nucleotides EX28DRAFT_1895 and EX28DRAFT_1896 are separated by 84 nucleotides EX28DRAFT_1896 and EX28DRAFT_1897 are separated by 232 nucleotides EX28DRAFT_1897 and EX28DRAFT_1898 are separated by 45 nucleotides EX28DRAFT_1898 and EX28DRAFT_1899 are separated by 48 nucleotides
EX28DRAFT_1894: EX28DRAFT_1894 - Uncharacterized component of anaerobic dehydrogenases, at 1,903,771 to 1,904,322
_1894
EX28DRAFT_1895: EX28DRAFT_1895 - Histidinol phosphatase and related hydrolases of the PHP family, at 1,904,344 to 1,905,081
_1895
EX28DRAFT_1896: EX28DRAFT_1896 - Phosphoglycerate dehydrogenase and related dehydrogenases, at 1,905,166 to 1,906,104
_1896
EX28DRAFT_1897: EX28DRAFT_1897 - tRNA, at 1,906,337 to 1,906,424
_1897
EX28DRAFT_1898: EX28DRAFT_1898 - RNA, at 1,906,470 to 1,906,607
_1898
EX28DRAFT_1899: EX28DRAFT_1899 - Transcriptional regulator, at 1,906,656 to 1,907,561
_1899
Position (kb)
1905
1906
1907 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1904.234 kb on - strand, within EX28DRAFT_1894 at 1904.279 kb on + strand at 1904.279 kb on + strand at 1904.280 kb on - strand at 1904.345 kb on + strand at 1904.416 kb on + strand at 1904.446 kb on - strand, within EX28DRAFT_1895 at 1904.446 kb on - strand, within EX28DRAFT_1895 at 1904.608 kb on - strand, within EX28DRAFT_1895 at 1904.608 kb on - strand, within EX28DRAFT_1895 at 1904.985 kb on + strand, within EX28DRAFT_1895 at 1904.985 kb on + strand, within EX28DRAFT_1895 at 1904.994 kb on + strand, within EX28DRAFT_1895 at 1905.014 kb on + strand at 1905.015 kb on - strand at 1905.015 kb on - strand at 1905.032 kb on + strand at 1905.032 kb on + strand at 1905.061 kb on - strand at 1905.061 kb on - strand at 1905.077 kb on + strand at 1905.136 kb on - strand at 1905.238 kb on + strand at 1905.239 kb on - strand at 1905.240 kb on + strand at 1905.240 kb on + strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.241 kb on - strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.242 kb on + strand at 1905.243 kb on - strand at 1905.346 kb on - strand, within EX28DRAFT_1896 at 1905.347 kb on + strand, within EX28DRAFT_1896 at 1905.348 kb on - strand, within EX28DRAFT_1896 at 1905.348 kb on - strand, within EX28DRAFT_1896 at 1905.443 kb on + strand, within EX28DRAFT_1896 at 1905.523 kb on - strand, within EX28DRAFT_1896 at 1905.693 kb on + strand, within EX28DRAFT_1896 at 1905.784 kb on + strand, within EX28DRAFT_1896 at 1905.800 kb on + strand, within EX28DRAFT_1896 at 1905.800 kb on + strand, within EX28DRAFT_1896 at 1905.983 kb on + strand, within EX28DRAFT_1896 at 1905.984 kb on - strand, within EX28DRAFT_1896 at 1906.064 kb on + strand at 1906.088 kb on + strand at 1906.096 kb on + strand at 1906.097 kb on - strand at 1906.101 kb on - strand at 1906.172 kb on + strand at 1906.218 kb on + strand at 1906.218 kb on + strand at 1906.254 kb on + strand at 1906.297 kb on + strand at 1906.429 kb on + strand at 1906.429 kb on + strand at 1906.429 kb on + strand at 1906.429 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.604 kb on + strand at 1906.606 kb on + strand at 1906.606 kb on + strand at 1906.607 kb on - strand at 1906.678 kb on + strand at 1906.958 kb on + strand, within EX28DRAFT_1899 at 1906.959 kb on - strand, within EX28DRAFT_1899 at 1906.964 kb on + strand, within EX28DRAFT_1899 at 1906.964 kb on + strand, within EX28DRAFT_1899 at 1906.965 kb on - strand, within EX28DRAFT_1899 at 1907.092 kb on - strand, within EX28DRAFT_1899 at 1907.095 kb on - strand, within EX28DRAFT_1899
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,904,234 - EX28DRAFT_1894 0.84 +0.6 1,904,279 + +0.4 1,904,279 + -0.4 1,904,280 - +0.6 1,904,345 + -0.5 1,904,416 + -0.3 1,904,446 - EX28DRAFT_1895 0.14 -0.2 1,904,446 - EX28DRAFT_1895 0.14 -0.6 1,904,608 - EX28DRAFT_1895 0.36 -0.2 1,904,608 - EX28DRAFT_1895 0.36 -0.1 1,904,985 + EX28DRAFT_1895 0.87 +0.3 1,904,985 + EX28DRAFT_1895 0.87 -1.2 1,904,994 + EX28DRAFT_1895 0.88 +0.7 1,905,014 + +0.1 1,905,015 - +2.4 1,905,015 - -0.0 1,905,032 + +0.9 1,905,032 + +0.4 1,905,061 - -0.6 1,905,061 - -0.0 1,905,077 + +0.5 1,905,136 - +0.1 1,905,238 + +0.1 1,905,239 - -0.1 1,905,240 + -0.4 1,905,240 + +0.8 1,905,241 - +0.2 1,905,241 - -0.4 1,905,241 - +1.1 1,905,241 - -0.8 1,905,241 - +0.6 1,905,241 - +0.0 1,905,242 + -0.5 1,905,242 + -2.0 1,905,242 + +0.4 1,905,242 + -0.3 1,905,242 + -3.3 1,905,242 + -0.3 1,905,243 - +2.6 1,905,346 - EX28DRAFT_1896 0.19 +1.2 1,905,347 + EX28DRAFT_1896 0.19 +0.8 1,905,348 - EX28DRAFT_1896 0.19 -0.5 1,905,348 - EX28DRAFT_1896 0.19 -0.7 1,905,443 + EX28DRAFT_1896 0.29 -0.4 1,905,523 - EX28DRAFT_1896 0.38 -1.0 1,905,693 + EX28DRAFT_1896 0.56 +0.1 1,905,784 + EX28DRAFT_1896 0.66 -0.6 1,905,800 + EX28DRAFT_1896 0.68 -0.1 1,905,800 + EX28DRAFT_1896 0.68 -1.7 1,905,983 + EX28DRAFT_1896 0.87 -1.0 1,905,984 - EX28DRAFT_1896 0.87 -1.0 1,906,064 + -4.2 1,906,088 + -0.2 1,906,096 + -2.4 1,906,097 - +1.0 1,906,101 - -0.1 1,906,172 + -1.0 1,906,218 + -0.1 1,906,218 + -0.0 1,906,254 + +0.2 1,906,297 + -0.3 1,906,429 + +0.3 1,906,429 + -0.2 1,906,429 + -0.3 1,906,429 + -0.5 1,906,604 + -0.1 1,906,604 + -1.2 1,906,604 + +0.6 1,906,604 + -2.0 1,906,604 + +0.4 1,906,604 + -1.0 1,906,606 + -0.0 1,906,606 + +0.4 1,906,607 - -1.1 1,906,678 + +0.9 1,906,958 + EX28DRAFT_1899 0.33 -0.0 1,906,959 - EX28DRAFT_1899 0.33 -0.1 1,906,964 + EX28DRAFT_1899 0.34 +0.4 1,906,964 + EX28DRAFT_1899 0.34 +1.3 1,906,965 - EX28DRAFT_1899 0.34 +0.6 1,907,092 - EX28DRAFT_1899 0.48 -0.0 1,907,095 - EX28DRAFT_1899 0.48 +1.4
Or see this region's nucleotide sequence