Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1684 and EX28DRAFT_1685 overlap by 26 nucleotides EX28DRAFT_1685 and EX28DRAFT_1686 are separated by 9 nucleotides EX28DRAFT_1686 and EX28DRAFT_1687 are separated by 8 nucleotides EX28DRAFT_1687 and EX28DRAFT_1688 are separated by 313 nucleotides
EX28DRAFT_1684: EX28DRAFT_1684 - FeS assembly protein SufD, at 1,696,439 to 1,697,710
_1684
EX28DRAFT_1685: EX28DRAFT_1685 - FeS assembly ATPase SufC, at 1,697,685 to 1,698,431
_1685
EX28DRAFT_1686: EX28DRAFT_1686 - FeS assembly protein SufB, at 1,698,441 to 1,699,931
_1686
EX28DRAFT_1687: EX28DRAFT_1687 - FeS assembly scaffold SufA, at 1,699,940 to 1,700,308
_1687
EX28DRAFT_1688: EX28DRAFT_1688 - MerR HTH family regulatory protein, at 1,700,622 to 1,701,350
_1688
Position (kb)
1698
1699
1700 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1697.558 kb on - strand, within EX28DRAFT_1684 at 1697.559 kb on + strand, within EX28DRAFT_1684 at 1697.579 kb on - strand, within EX28DRAFT_1684 at 1697.686 kb on + strand at 1697.687 kb on - strand at 1697.687 kb on - strand at 1697.700 kb on - strand at 1697.762 kb on + strand, within EX28DRAFT_1685 at 1697.762 kb on + strand, within EX28DRAFT_1685 at 1697.803 kb on + strand, within EX28DRAFT_1685 at 1697.803 kb on + strand, within EX28DRAFT_1685 at 1697.928 kb on + strand, within EX28DRAFT_1685 at 1697.928 kb on + strand, within EX28DRAFT_1685 at 1697.972 kb on + strand, within EX28DRAFT_1685 at 1698.275 kb on - strand, within EX28DRAFT_1685 at 1698.399 kb on + strand at 1698.427 kb on - strand at 1698.590 kb on - strand, within EX28DRAFT_1686 at 1698.667 kb on + strand, within EX28DRAFT_1686 at 1698.677 kb on + strand, within EX28DRAFT_1686 at 1698.678 kb on - strand, within EX28DRAFT_1686 at 1699.088 kb on - strand, within EX28DRAFT_1686 at 1699.088 kb on - strand, within EX28DRAFT_1686 at 1699.144 kb on + strand, within EX28DRAFT_1686 at 1699.258 kb on + strand, within EX28DRAFT_1686 at 1699.258 kb on + strand, within EX28DRAFT_1686 at 1699.300 kb on + strand, within EX28DRAFT_1686 at 1699.301 kb on - strand, within EX28DRAFT_1686 at 1699.301 kb on - strand, within EX28DRAFT_1686 at 1699.301 kb on - strand, within EX28DRAFT_1686 at 1699.304 kb on + strand, within EX28DRAFT_1686 at 1699.305 kb on - strand, within EX28DRAFT_1686 at 1699.305 kb on - strand, within EX28DRAFT_1686 at 1699.305 kb on - strand, within EX28DRAFT_1686 at 1699.381 kb on + strand, within EX28DRAFT_1686 at 1699.663 kb on + strand, within EX28DRAFT_1686 at 1699.664 kb on - strand, within EX28DRAFT_1686 at 1699.679 kb on - strand, within EX28DRAFT_1686 at 1699.870 kb on + strand at 1699.870 kb on + strand at 1699.870 kb on + strand at 1699.870 kb on + strand at 1699.870 kb on + strand at 1699.871 kb on - strand at 1699.871 kb on - strand at 1699.871 kb on - strand at 1699.899 kb on + strand at 1699.899 kb on + strand at 1699.900 kb on - strand at 1699.900 kb on - strand at 1699.900 kb on - strand at 1699.900 kb on - strand at 1699.919 kb on + strand at 1699.920 kb on - strand at 1699.922 kb on + strand at 1699.931 kb on + strand at 1699.932 kb on - strand at 1699.932 kb on - strand at 1699.932 kb on - strand at 1699.932 kb on - strand at 1699.932 kb on - strand at 1699.941 kb on + strand at 1699.942 kb on - strand at 1700.186 kb on + strand, within EX28DRAFT_1687 at 1700.227 kb on + strand, within EX28DRAFT_1687 at 1700.228 kb on - strand, within EX28DRAFT_1687 at 1700.326 kb on + strand at 1700.341 kb on + strand at 1700.420 kb on - strand at 1700.434 kb on + strand at 1700.435 kb on - strand at 1700.588 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,697,558 - EX28DRAFT_1684 0.88 -0.0 1,697,559 + EX28DRAFT_1684 0.88 +1.2 1,697,579 - EX28DRAFT_1684 0.90 +0.4 1,697,686 + +1.8 1,697,687 - -0.9 1,697,687 - -1.0 1,697,700 - -2.0 1,697,762 + EX28DRAFT_1685 0.10 +3.1 1,697,762 + EX28DRAFT_1685 0.10 +1.1 1,697,803 + EX28DRAFT_1685 0.16 +0.7 1,697,803 + EX28DRAFT_1685 0.16 +0.6 1,697,928 + EX28DRAFT_1685 0.33 +0.3 1,697,928 + EX28DRAFT_1685 0.33 -0.2 1,697,972 + EX28DRAFT_1685 0.38 +0.2 1,698,275 - EX28DRAFT_1685 0.79 +0.6 1,698,399 + +1.6 1,698,427 - +1.0 1,698,590 - EX28DRAFT_1686 0.10 +0.3 1,698,667 + EX28DRAFT_1686 0.15 +0.1 1,698,677 + EX28DRAFT_1686 0.16 +2.5 1,698,678 - EX28DRAFT_1686 0.16 +0.7 1,699,088 - EX28DRAFT_1686 0.43 +1.2 1,699,088 - EX28DRAFT_1686 0.43 +1.2 1,699,144 + EX28DRAFT_1686 0.47 +0.4 1,699,258 + EX28DRAFT_1686 0.55 -1.8 1,699,258 + EX28DRAFT_1686 0.55 +0.2 1,699,300 + EX28DRAFT_1686 0.58 +1.3 1,699,301 - EX28DRAFT_1686 0.58 +0.4 1,699,301 - EX28DRAFT_1686 0.58 +0.1 1,699,301 - EX28DRAFT_1686 0.58 +0.0 1,699,304 + EX28DRAFT_1686 0.58 -0.1 1,699,305 - EX28DRAFT_1686 0.58 +0.9 1,699,305 - EX28DRAFT_1686 0.58 +0.3 1,699,305 - EX28DRAFT_1686 0.58 +0.3 1,699,381 + EX28DRAFT_1686 0.63 +1.5 1,699,663 + EX28DRAFT_1686 0.82 -0.2 1,699,664 - EX28DRAFT_1686 0.82 -0.6 1,699,679 - EX28DRAFT_1686 0.83 -0.0 1,699,870 + +0.3 1,699,870 + +0.4 1,699,870 + +0.1 1,699,870 + +0.3 1,699,870 + +1.7 1,699,871 - +1.2 1,699,871 - -0.2 1,699,871 - +0.6 1,699,899 + +0.2 1,699,899 + -1.2 1,699,900 - +0.8 1,699,900 - -0.2 1,699,900 - +0.3 1,699,900 - +0.2 1,699,919 + +1.6 1,699,920 - +0.2 1,699,922 + +1.7 1,699,931 + +1.9 1,699,932 - +0.1 1,699,932 - +0.3 1,699,932 - +0.4 1,699,932 - -0.2 1,699,932 - +0.8 1,699,941 + +0.4 1,699,942 - -0.4 1,700,186 + EX28DRAFT_1687 0.67 -0.9 1,700,227 + EX28DRAFT_1687 0.78 -0.6 1,700,228 - EX28DRAFT_1687 0.78 +0.4 1,700,326 + -0.4 1,700,341 + +0.1 1,700,420 - -0.1 1,700,434 + -1.6 1,700,435 - +0.1 1,700,588 + -0.1
Or see this region's nucleotide sequence