Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1642 and EX28DRAFT_1643 overlap by 22 nucleotides EX28DRAFT_1643 and EX28DRAFT_1644 are separated by 80 nucleotides
EX28DRAFT_1642: EX28DRAFT_1642 - Predicted membrane protein, at 1,658,544 to 1,660,577
_1642
EX28DRAFT_1643: EX28DRAFT_1643 - Cu/Zn superoxide dismutase, at 1,660,556 to 1,661,074
_1643
EX28DRAFT_1644: EX28DRAFT_1644 - Predicted oxidoreductase, at 1,661,155 to 1,662,051
_1644
Position (kb)
1660
1661
1662 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1659.680 kb on + strand, within EX28DRAFT_1642 at 1659.741 kb on + strand, within EX28DRAFT_1642 at 1659.741 kb on + strand, within EX28DRAFT_1642 at 1659.773 kb on + strand, within EX28DRAFT_1642 at 1659.841 kb on + strand, within EX28DRAFT_1642 at 1659.842 kb on - strand, within EX28DRAFT_1642 at 1659.847 kb on + strand, within EX28DRAFT_1642 at 1659.848 kb on - strand, within EX28DRAFT_1642 at 1659.848 kb on - strand, within EX28DRAFT_1642 at 1659.972 kb on - strand, within EX28DRAFT_1642 at 1660.075 kb on + strand, within EX28DRAFT_1642 at 1660.075 kb on + strand, within EX28DRAFT_1642 at 1660.123 kb on + strand, within EX28DRAFT_1642 at 1660.123 kb on + strand, within EX28DRAFT_1642 at 1660.123 kb on + strand, within EX28DRAFT_1642 at 1660.194 kb on + strand, within EX28DRAFT_1642 at 1660.195 kb on - strand, within EX28DRAFT_1642 at 1660.372 kb on + strand, within EX28DRAFT_1642 at 1660.480 kb on + strand at 1660.481 kb on - strand at 1660.487 kb on + strand at 1660.487 kb on + strand at 1660.488 kb on - strand at 1660.490 kb on - strand at 1660.490 kb on - strand at 1660.490 kb on - strand at 1660.618 kb on + strand, within EX28DRAFT_1643 at 1660.619 kb on - strand, within EX28DRAFT_1643 at 1661.074 kb on + strand at 1661.075 kb on - strand at 1661.104 kb on + strand at 1661.110 kb on + strand at 1661.312 kb on + strand, within EX28DRAFT_1644 at 1661.312 kb on + strand, within EX28DRAFT_1644 at 1661.756 kb on + strand, within EX28DRAFT_1644 at 1661.889 kb on - strand, within EX28DRAFT_1644 at 1661.893 kb on - strand, within EX28DRAFT_1644 at 1661.967 kb on + strand at 1661.967 kb on + strand at 1661.968 kb on - strand at 1661.968 kb on - strand at 1661.968 kb on - strand at 1661.990 kb on + strand at 1661.991 kb on - strand at 1661.999 kb on - strand at 1662.036 kb on + strand at 1662.071 kb on + strand at 1662.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,659,680 + EX28DRAFT_1642 0.56 -2.5 1,659,741 + EX28DRAFT_1642 0.59 -3.4 1,659,741 + EX28DRAFT_1642 0.59 +0.0 1,659,773 + EX28DRAFT_1642 0.60 -0.4 1,659,841 + EX28DRAFT_1642 0.64 -1.2 1,659,842 - EX28DRAFT_1642 0.64 -1.8 1,659,847 + EX28DRAFT_1642 0.64 +1.9 1,659,848 - EX28DRAFT_1642 0.64 +0.2 1,659,848 - EX28DRAFT_1642 0.64 -0.6 1,659,972 - EX28DRAFT_1642 0.70 +0.4 1,660,075 + EX28DRAFT_1642 0.75 -0.1 1,660,075 + EX28DRAFT_1642 0.75 +0.1 1,660,123 + EX28DRAFT_1642 0.78 +0.1 1,660,123 + EX28DRAFT_1642 0.78 -0.8 1,660,123 + EX28DRAFT_1642 0.78 +0.1 1,660,194 + EX28DRAFT_1642 0.81 -0.3 1,660,195 - EX28DRAFT_1642 0.81 +0.1 1,660,372 + EX28DRAFT_1642 0.90 +0.6 1,660,480 + +0.9 1,660,481 - -0.3 1,660,487 + -0.5 1,660,487 + +0.2 1,660,488 - -0.2 1,660,490 - -0.5 1,660,490 - -0.1 1,660,490 - +0.9 1,660,618 + EX28DRAFT_1643 0.12 +0.4 1,660,619 - EX28DRAFT_1643 0.12 +0.5 1,661,074 + +1.5 1,661,075 - +0.8 1,661,104 + +1.5 1,661,110 + +1.0 1,661,312 + EX28DRAFT_1644 0.18 -1.8 1,661,312 + EX28DRAFT_1644 0.18 -1.3 1,661,756 + EX28DRAFT_1644 0.67 -0.6 1,661,889 - EX28DRAFT_1644 0.82 -4.4 1,661,893 - EX28DRAFT_1644 0.82 -2.0 1,661,967 + +2.0 1,661,967 + +0.7 1,661,968 - -2.5 1,661,968 - -3.2 1,661,968 - -0.9 1,661,990 + -0.8 1,661,991 - +0.5 1,661,999 - -1.6 1,662,036 + -0.1 1,662,071 + -0.2 1,662,071 + -4.3
Or see this region's nucleotide sequence