Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1540 and EX28DRAFT_1541 are separated by 134 nucleotides EX28DRAFT_1541 and EX28DRAFT_1542 are separated by 109 nucleotides EX28DRAFT_1542 and EX28DRAFT_1543 are separated by 120 nucleotides
EX28DRAFT_1540: EX28DRAFT_1540 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 1,555,366 to 1,556,118
_1540
EX28DRAFT_1541: EX28DRAFT_1541 - hypothetical protein, at 1,556,253 to 1,556,447
_1541
EX28DRAFT_1542: EX28DRAFT_1542 - Universal stress protein UspA and related nucleotide-binding proteins, at 1,556,557 to 1,557,507
_1542
EX28DRAFT_1543: EX28DRAFT_1543 - NAD/NADP transhydrogenase beta subunit, at 1,557,628 to 1,559,001
_1543
Position (kb) 1556
1557
1558 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1555.600 kb on + strand, within EX28DRAFT_1540 at 1555.600 kb on + strand, within EX28DRAFT_1540 at 1555.884 kb on + strand, within EX28DRAFT_1540 at 1555.885 kb on - strand, within EX28DRAFT_1540 at 1555.972 kb on + strand, within EX28DRAFT_1540 at 1555.973 kb on - strand, within EX28DRAFT_1540 at 1556.050 kb on - strand at 1556.054 kb on - strand at 1556.174 kb on + strand at 1556.208 kb on + strand at 1556.208 kb on + strand at 1556.209 kb on - strand at 1556.303 kb on + strand, within EX28DRAFT_1541 at 1556.303 kb on + strand, within EX28DRAFT_1541 at 1556.356 kb on - strand, within EX28DRAFT_1541 at 1556.406 kb on - strand, within EX28DRAFT_1541 at 1556.434 kb on + strand at 1556.503 kb on - strand at 1556.503 kb on - strand at 1556.505 kb on - strand at 1556.505 kb on - strand at 1556.506 kb on + strand at 1556.506 kb on + strand at 1556.507 kb on - strand at 1556.507 kb on - strand at 1556.524 kb on - strand at 1556.541 kb on + strand at 1556.542 kb on - strand at 1556.582 kb on + strand at 1556.586 kb on + strand at 1556.633 kb on - strand at 1556.633 kb on - strand at 1556.692 kb on + strand, within EX28DRAFT_1542 at 1556.788 kb on + strand, within EX28DRAFT_1542 at 1556.788 kb on + strand, within EX28DRAFT_1542 at 1556.791 kb on + strand, within EX28DRAFT_1542 at 1556.792 kb on - strand, within EX28DRAFT_1542 at 1556.818 kb on - strand, within EX28DRAFT_1542 at 1556.820 kb on + strand, within EX28DRAFT_1542 at 1556.820 kb on + strand, within EX28DRAFT_1542 at 1556.964 kb on + strand, within EX28DRAFT_1542 at 1557.124 kb on - strand, within EX28DRAFT_1542 at 1557.585 kb on - strand at 1558.236 kb on + strand, within EX28DRAFT_1543 at 1558.247 kb on + strand, within EX28DRAFT_1543 at 1558.247 kb on + strand, within EX28DRAFT_1543 at 1558.247 kb on + strand, within EX28DRAFT_1543 at 1558.248 kb on - strand, within EX28DRAFT_1543 at 1558.248 kb on - strand, within EX28DRAFT_1543 at 1558.248 kb on - strand, within EX28DRAFT_1543 at 1558.314 kb on + strand, within EX28DRAFT_1543 at 1558.347 kb on - strand, within EX28DRAFT_1543 at 1558.499 kb on - strand, within EX28DRAFT_1543
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,555,600 + EX28DRAFT_1540 0.31 +0.9 1,555,600 + EX28DRAFT_1540 0.31 -0.7 1,555,884 + EX28DRAFT_1540 0.69 -0.1 1,555,885 - EX28DRAFT_1540 0.69 +0.2 1,555,972 + EX28DRAFT_1540 0.80 +2.1 1,555,973 - EX28DRAFT_1540 0.81 -0.2 1,556,050 - +0.9 1,556,054 - +1.1 1,556,174 + +1.6 1,556,208 + -2.9 1,556,208 + +2.0 1,556,209 - +0.8 1,556,303 + EX28DRAFT_1541 0.26 -0.2 1,556,303 + EX28DRAFT_1541 0.26 +1.3 1,556,356 - EX28DRAFT_1541 0.53 +0.5 1,556,406 - EX28DRAFT_1541 0.78 +2.2 1,556,434 + +0.0 1,556,503 - -0.7 1,556,503 - +0.2 1,556,505 - -0.1 1,556,505 - -2.1 1,556,506 + -2.6 1,556,506 + -1.9 1,556,507 - +0.6 1,556,507 - -0.2 1,556,524 - -2.9 1,556,541 + -1.9 1,556,542 - +1.4 1,556,582 + -0.3 1,556,586 + +0.9 1,556,633 - +1.1 1,556,633 - -1.3 1,556,692 + EX28DRAFT_1542 0.14 +0.1 1,556,788 + EX28DRAFT_1542 0.24 +0.1 1,556,788 + EX28DRAFT_1542 0.24 +0.3 1,556,791 + EX28DRAFT_1542 0.25 +0.8 1,556,792 - EX28DRAFT_1542 0.25 +0.9 1,556,818 - EX28DRAFT_1542 0.27 -1.3 1,556,820 + EX28DRAFT_1542 0.28 -0.3 1,556,820 + EX28DRAFT_1542 0.28 +0.3 1,556,964 + EX28DRAFT_1542 0.43 -0.6 1,557,124 - EX28DRAFT_1542 0.60 -0.1 1,557,585 - -0.7 1,558,236 + EX28DRAFT_1543 0.44 -1.9 1,558,247 + EX28DRAFT_1543 0.45 +0.6 1,558,247 + EX28DRAFT_1543 0.45 -1.7 1,558,247 + EX28DRAFT_1543 0.45 +0.9 1,558,248 - EX28DRAFT_1543 0.45 -0.5 1,558,248 - EX28DRAFT_1543 0.45 -1.3 1,558,248 - EX28DRAFT_1543 0.45 -2.6 1,558,314 + EX28DRAFT_1543 0.50 -0.8 1,558,347 - EX28DRAFT_1543 0.52 +0.8 1,558,499 - EX28DRAFT_1543 0.63 -0.1
Or see this region's nucleotide sequence