Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1249 and EX28DRAFT_1250 are separated by 11 nucleotides EX28DRAFT_1250 and EX28DRAFT_1251 are separated by 9 nucleotides EX28DRAFT_1251 and EX28DRAFT_1252 are separated by 132 nucleotides EX28DRAFT_1252 and EX28DRAFT_1253 are separated by 64 nucleotides
EX28DRAFT_1249: EX28DRAFT_1249 - amidohydrolase, PncC family, at 1,266,870 to 1,267,388
_1249
EX28DRAFT_1250: EX28DRAFT_1250 - Protein of unknown function (DUF421), at 1,267,400 to 1,267,846
_1250
EX28DRAFT_1251: EX28DRAFT_1251 - hypothetical protein, at 1,267,856 to 1,268,365
_1251
EX28DRAFT_1252: EX28DRAFT_1252 - TIGR00156 family protein, at 1,268,498 to 1,268,887
_1252
EX28DRAFT_1253: EX28DRAFT_1253 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,268,952 to 1,269,836
_1253
Position (kb)
1267
1268
1269 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1266.865 kb on + strand at 1266.866 kb on - strand at 1266.866 kb on - strand at 1267.359 kb on - strand at 1267.456 kb on + strand, within EX28DRAFT_1250 at 1267.815 kb on + strand at 1267.820 kb on - strand at 1267.867 kb on + strand at 1267.990 kb on - strand, within EX28DRAFT_1251 at 1268.023 kb on + strand, within EX28DRAFT_1251 at 1268.024 kb on - strand, within EX28DRAFT_1251 at 1268.024 kb on - strand, within EX28DRAFT_1251 at 1268.094 kb on + strand, within EX28DRAFT_1251 at 1268.094 kb on + strand, within EX28DRAFT_1251 at 1268.094 kb on + strand, within EX28DRAFT_1251 at 1268.094 kb on + strand, within EX28DRAFT_1251 at 1268.095 kb on - strand, within EX28DRAFT_1251 at 1268.095 kb on - strand, within EX28DRAFT_1251 at 1268.095 kb on - strand, within EX28DRAFT_1251 at 1268.095 kb on - strand, within EX28DRAFT_1251 at 1268.166 kb on + strand, within EX28DRAFT_1251 at 1268.167 kb on - strand, within EX28DRAFT_1251 at 1268.171 kb on + strand, within EX28DRAFT_1251 at 1268.172 kb on - strand, within EX28DRAFT_1251 at 1268.272 kb on + strand, within EX28DRAFT_1251 at 1268.442 kb on + strand at 1268.466 kb on + strand at 1268.781 kb on + strand, within EX28DRAFT_1252 at 1268.951 kb on + strand at 1268.951 kb on + strand at 1268.964 kb on - strand at 1268.973 kb on - strand at 1268.973 kb on - strand at 1269.109 kb on + strand, within EX28DRAFT_1253 at 1269.110 kb on - strand, within EX28DRAFT_1253 at 1269.156 kb on + strand, within EX28DRAFT_1253 at 1269.185 kb on + strand, within EX28DRAFT_1253 at 1269.186 kb on - strand, within EX28DRAFT_1253 at 1269.190 kb on - strand, within EX28DRAFT_1253
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,266,865 + -4.3 1,266,866 - -0.3 1,266,866 - -2.2 1,267,359 - +0.2 1,267,456 + EX28DRAFT_1250 0.13 +0.6 1,267,815 + -0.2 1,267,820 - +0.8 1,267,867 + -0.4 1,267,990 - EX28DRAFT_1251 0.26 +0.7 1,268,023 + EX28DRAFT_1251 0.33 +1.5 1,268,024 - EX28DRAFT_1251 0.33 -3.0 1,268,024 - EX28DRAFT_1251 0.33 +0.4 1,268,094 + EX28DRAFT_1251 0.47 -0.2 1,268,094 + EX28DRAFT_1251 0.47 +1.6 1,268,094 + EX28DRAFT_1251 0.47 +1.6 1,268,094 + EX28DRAFT_1251 0.47 +0.5 1,268,095 - EX28DRAFT_1251 0.47 +1.4 1,268,095 - EX28DRAFT_1251 0.47 +0.5 1,268,095 - EX28DRAFT_1251 0.47 -0.8 1,268,095 - EX28DRAFT_1251 0.47 -0.6 1,268,166 + EX28DRAFT_1251 0.61 +1.1 1,268,167 - EX28DRAFT_1251 0.61 +0.7 1,268,171 + EX28DRAFT_1251 0.62 -0.5 1,268,172 - EX28DRAFT_1251 0.62 -0.2 1,268,272 + EX28DRAFT_1251 0.82 -0.6 1,268,442 + +0.8 1,268,466 + +0.1 1,268,781 + EX28DRAFT_1252 0.73 +1.0 1,268,951 + -1.7 1,268,951 + +0.3 1,268,964 - -0.5 1,268,973 - +0.5 1,268,973 - -1.3 1,269,109 + EX28DRAFT_1253 0.18 +0.3 1,269,110 - EX28DRAFT_1253 0.18 +0.9 1,269,156 + EX28DRAFT_1253 0.23 +0.1 1,269,185 + EX28DRAFT_1253 0.26 -0.0 1,269,186 - EX28DRAFT_1253 0.26 +0.2 1,269,190 - EX28DRAFT_1253 0.27 +1.7
Or see this region's nucleotide sequence