Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0532 and EX28DRAFT_0533 are separated by 40 nucleotides EX28DRAFT_0533 and EX28DRAFT_0534 overlap by 7 nucleotides EX28DRAFT_0534 and EX28DRAFT_0535 overlap by 19 nucleotides
EX28DRAFT_0532: EX28DRAFT_0532 - Chain length determinant protein, at 527,068 to 528,048
_0532
EX28DRAFT_0533: EX28DRAFT_0533 - phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 528,089 to 528,700
_0533
EX28DRAFT_0534: EX28DRAFT_0534 - imidazoleglycerol phosphate synthase, cyclase subunit, at 528,694 to 529,470
_0534
EX28DRAFT_0535: EX28DRAFT_0535 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC 5.3.1.16), at 529,452 to 530,189
_0535
Position (kb) 528
529 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 527.127 kb on - strand at 527.135 kb on + strand at 527.190 kb on + strand, within EX28DRAFT_0532 at 527.213 kb on + strand, within EX28DRAFT_0532 at 527.284 kb on + strand, within EX28DRAFT_0532 at 527.285 kb on - strand, within EX28DRAFT_0532 at 527.285 kb on - strand, within EX28DRAFT_0532 at 527.365 kb on - strand, within EX28DRAFT_0532 at 527.412 kb on - strand, within EX28DRAFT_0532 at 527.424 kb on + strand, within EX28DRAFT_0532 at 527.476 kb on - strand, within EX28DRAFT_0532 at 527.518 kb on + strand, within EX28DRAFT_0532 at 527.518 kb on + strand, within EX28DRAFT_0532 at 527.615 kb on + strand, within EX28DRAFT_0532 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.830 kb on + strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532 at 527.886 kb on + strand, within EX28DRAFT_0532 at 527.887 kb on - strand, within EX28DRAFT_0532 at 527.896 kb on + strand, within EX28DRAFT_0532 at 527.999 kb on + strand at 528.023 kb on + strand at 528.031 kb on + strand at 528.075 kb on + strand at 528.168 kb on + strand, within EX28DRAFT_0533 at 528.168 kb on + strand, within EX28DRAFT_0533 at 528.212 kb on + strand, within EX28DRAFT_0533 at 528.213 kb on - strand, within EX28DRAFT_0533 at 528.213 kb on - strand, within EX28DRAFT_0533 at 528.265 kb on + strand, within EX28DRAFT_0533 at 528.266 kb on - strand, within EX28DRAFT_0533 at 528.301 kb on - strand, within EX28DRAFT_0533 at 528.301 kb on - strand, within EX28DRAFT_0533 at 528.592 kb on + strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.595 kb on - strand, within EX28DRAFT_0533 at 528.731 kb on + strand at 528.750 kb on + strand at 528.751 kb on - strand at 528.751 kb on - strand at 529.053 kb on - strand, within EX28DRAFT_0534 at 529.074 kb on - strand, within EX28DRAFT_0534 at 529.098 kb on + strand, within EX28DRAFT_0534 at 529.099 kb on - strand, within EX28DRAFT_0534 at 529.177 kb on - strand, within EX28DRAFT_0534 at 529.322 kb on + strand, within EX28DRAFT_0534 at 529.323 kb on - strand, within EX28DRAFT_0534 at 529.435 kb on + strand at 529.450 kb on + strand at 529.450 kb on + strand at 529.450 kb on + strand at 529.451 kb on - strand at 529.451 kb on - strand at 529.490 kb on + strand at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.567 kb on - strand, within EX28DRAFT_0535
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 527,127 - +0.7 527,135 + +0.6 527,190 + EX28DRAFT_0532 0.12 +0.8 527,213 + EX28DRAFT_0532 0.15 -0.3 527,284 + EX28DRAFT_0532 0.22 -1.0 527,285 - EX28DRAFT_0532 0.22 +1.1 527,285 - EX28DRAFT_0532 0.22 +0.8 527,365 - EX28DRAFT_0532 0.30 +0.4 527,412 - EX28DRAFT_0532 0.35 -1.8 527,424 + EX28DRAFT_0532 0.36 -0.4 527,476 - EX28DRAFT_0532 0.42 +0.1 527,518 + EX28DRAFT_0532 0.46 +1.0 527,518 + EX28DRAFT_0532 0.46 +0.7 527,615 + EX28DRAFT_0532 0.56 -0.3 527,735 + EX28DRAFT_0532 0.68 -3.1 527,735 + EX28DRAFT_0532 0.68 -0.5 527,830 + EX28DRAFT_0532 0.78 +0.6 527,874 - EX28DRAFT_0532 0.82 +0.4 527,874 - EX28DRAFT_0532 0.82 +0.6 527,886 + EX28DRAFT_0532 0.83 -2.3 527,887 - EX28DRAFT_0532 0.83 +1.8 527,896 + EX28DRAFT_0532 0.84 -2.8 527,999 + +2.4 528,023 + -0.5 528,031 + -0.1 528,075 + +1.0 528,168 + EX28DRAFT_0533 0.13 -1.8 528,168 + EX28DRAFT_0533 0.13 -1.3 528,212 + EX28DRAFT_0533 0.20 -5.2 528,213 - EX28DRAFT_0533 0.20 -1.4 528,213 - EX28DRAFT_0533 0.20 -3.7 528,265 + EX28DRAFT_0533 0.29 -2.7 528,266 - EX28DRAFT_0533 0.29 -1.9 528,301 - EX28DRAFT_0533 0.35 -2.5 528,301 - EX28DRAFT_0533 0.35 -2.0 528,592 + EX28DRAFT_0533 0.82 -2.0 528,593 - EX28DRAFT_0533 0.82 -3.7 528,593 - EX28DRAFT_0533 0.82 -0.4 528,593 - EX28DRAFT_0533 0.82 -2.8 528,593 - EX28DRAFT_0533 0.82 -5.0 528,593 - EX28DRAFT_0533 0.82 -4.2 528,595 - EX28DRAFT_0533 0.83 -3.2 528,731 + -2.6 528,750 + -1.7 528,751 - -3.4 528,751 - -4.9 529,053 - EX28DRAFT_0534 0.46 -6.5 529,074 - EX28DRAFT_0534 0.49 -3.5 529,098 + EX28DRAFT_0534 0.52 -2.7 529,099 - EX28DRAFT_0534 0.52 -3.0 529,177 - EX28DRAFT_0534 0.62 +0.0 529,322 + EX28DRAFT_0534 0.81 -2.3 529,323 - EX28DRAFT_0534 0.81 -3.9 529,435 + -3.4 529,450 + -2.7 529,450 + -3.4 529,450 + -2.5 529,451 - -2.5 529,451 - -1.9 529,490 + -3.3 529,566 + EX28DRAFT_0535 0.15 -2.9 529,566 + EX28DRAFT_0535 0.15 -5.9 529,566 + EX28DRAFT_0535 0.15 -2.3 529,567 - EX28DRAFT_0535 0.16 -1.7
Or see this region's nucleotide sequence