Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0410 and EX28DRAFT_0411 are separated by 124 nucleotides EX28DRAFT_0411 and EX28DRAFT_0412 are separated by 126 nucleotides EX28DRAFT_0412 and EX28DRAFT_0413 are separated by 129 nucleotides EX28DRAFT_0413 and EX28DRAFT_0414 overlap by 4 nucleotides
EX28DRAFT_0410: EX28DRAFT_0410 - Glutamine synthetase, at 388,051 to 389,439
_0410
EX28DRAFT_0411: EX28DRAFT_0411 - Uncharacterized membrane protein, at 389,564 to 390,280
_0411
EX28DRAFT_0412: EX28DRAFT_0412 - cytidine deaminase (EC 3.5.4.5), at 390,407 to 391,291
_0412
EX28DRAFT_0413: EX28DRAFT_0413 - TIGR00659 family protein, at 391,421 to 392,116
_0413
EX28DRAFT_0414: EX28DRAFT_0414 - Putative effector of murein hydrolase LrgA, at 392,113 to 392,508
_0414
Position (kb) 390
391
392 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 389.440 kb on + strand at 389.441 kb on - strand at 389.494 kb on - strand at 389.549 kb on - strand at 389.550 kb on + strand at 389.551 kb on - strand at 389.566 kb on - strand at 389.585 kb on - strand at 389.672 kb on + strand, within EX28DRAFT_0411 at 389.700 kb on + strand, within EX28DRAFT_0411 at 389.700 kb on + strand, within EX28DRAFT_0411 at 389.701 kb on - strand, within EX28DRAFT_0411 at 389.759 kb on - strand, within EX28DRAFT_0411 at 389.781 kb on + strand, within EX28DRAFT_0411 at 389.804 kb on - strand, within EX28DRAFT_0411 at 390.030 kb on + strand, within EX28DRAFT_0411 at 390.030 kb on + strand, within EX28DRAFT_0411 at 390.030 kb on + strand, within EX28DRAFT_0411 at 390.051 kb on + strand, within EX28DRAFT_0411 at 390.052 kb on - strand, within EX28DRAFT_0411 at 390.088 kb on - strand, within EX28DRAFT_0411 at 390.114 kb on + strand, within EX28DRAFT_0411 at 390.115 kb on - strand, within EX28DRAFT_0411 at 390.180 kb on + strand, within EX28DRAFT_0411 at 390.181 kb on - strand, within EX28DRAFT_0411 at 390.237 kb on - strand at 390.237 kb on - strand at 390.318 kb on + strand at 390.419 kb on + strand at 390.675 kb on + strand, within EX28DRAFT_0412 at 390.814 kb on - strand, within EX28DRAFT_0412 at 390.814 kb on - strand, within EX28DRAFT_0412 at 390.887 kb on - strand, within EX28DRAFT_0412 at 390.887 kb on - strand, within EX28DRAFT_0412 at 390.915 kb on + strand, within EX28DRAFT_0412 at 390.916 kb on - strand, within EX28DRAFT_0412 at 390.916 kb on - strand, within EX28DRAFT_0412 at 391.024 kb on + strand, within EX28DRAFT_0412 at 391.038 kb on + strand, within EX28DRAFT_0412 at 391.038 kb on + strand, within EX28DRAFT_0412 at 391.038 kb on + strand, within EX28DRAFT_0412 at 391.306 kb on - strand at 391.507 kb on + strand, within EX28DRAFT_0413 at 391.525 kb on - strand, within EX28DRAFT_0413 at 391.598 kb on - strand, within EX28DRAFT_0413 at 391.845 kb on - strand, within EX28DRAFT_0413 at 391.884 kb on + strand, within EX28DRAFT_0413 at 391.884 kb on + strand, within EX28DRAFT_0413 at 391.886 kb on + strand, within EX28DRAFT_0413 at 391.886 kb on + strand, within EX28DRAFT_0413 at 391.953 kb on + strand, within EX28DRAFT_0413 at 392.104 kb on + strand at 392.142 kb on + strand at 392.255 kb on + strand, within EX28DRAFT_0414
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 389,440 + -1.8 389,441 - +0.6 389,494 - -3.0 389,549 - -0.4 389,550 + -0.1 389,551 - -0.4 389,566 - +0.6 389,585 - -0.4 389,672 + EX28DRAFT_0411 0.15 +0.0 389,700 + EX28DRAFT_0411 0.19 +0.1 389,700 + EX28DRAFT_0411 0.19 -0.8 389,701 - EX28DRAFT_0411 0.19 -1.0 389,759 - EX28DRAFT_0411 0.27 -0.6 389,781 + EX28DRAFT_0411 0.30 -0.4 389,804 - EX28DRAFT_0411 0.33 -1.4 390,030 + EX28DRAFT_0411 0.65 -0.1 390,030 + EX28DRAFT_0411 0.65 +0.6 390,030 + EX28DRAFT_0411 0.65 -1.1 390,051 + EX28DRAFT_0411 0.68 -1.2 390,052 - EX28DRAFT_0411 0.68 +2.2 390,088 - EX28DRAFT_0411 0.73 -0.9 390,114 + EX28DRAFT_0411 0.77 +2.1 390,115 - EX28DRAFT_0411 0.77 -2.4 390,180 + EX28DRAFT_0411 0.86 -0.3 390,181 - EX28DRAFT_0411 0.86 +0.1 390,237 - +1.2 390,237 - -0.2 390,318 + -2.0 390,419 + +1.9 390,675 + EX28DRAFT_0412 0.30 +0.3 390,814 - EX28DRAFT_0412 0.46 -0.0 390,814 - EX28DRAFT_0412 0.46 +0.3 390,887 - EX28DRAFT_0412 0.54 -1.9 390,887 - EX28DRAFT_0412 0.54 -0.9 390,915 + EX28DRAFT_0412 0.57 +0.0 390,916 - EX28DRAFT_0412 0.58 -0.3 390,916 - EX28DRAFT_0412 0.58 +1.7 391,024 + EX28DRAFT_0412 0.70 -0.2 391,038 + EX28DRAFT_0412 0.71 +0.4 391,038 + EX28DRAFT_0412 0.71 -0.0 391,038 + EX28DRAFT_0412 0.71 -0.3 391,306 - +0.1 391,507 + EX28DRAFT_0413 0.12 -0.2 391,525 - EX28DRAFT_0413 0.15 -0.3 391,598 - EX28DRAFT_0413 0.25 -3.2 391,845 - EX28DRAFT_0413 0.61 -0.1 391,884 + EX28DRAFT_0413 0.67 -0.1 391,884 + EX28DRAFT_0413 0.67 +0.6 391,886 + EX28DRAFT_0413 0.67 -0.1 391,886 + EX28DRAFT_0413 0.67 -0.3 391,953 + EX28DRAFT_0413 0.76 +0.9 392,104 + -0.9 392,142 + +1.1 392,255 + EX28DRAFT_0414 0.36 +0.4
Or see this region's nucleotide sequence