Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0220 and EX28DRAFT_0221 overlap by 4 nucleotides EX28DRAFT_0221 and EX28DRAFT_0222 are separated by 186 nucleotides EX28DRAFT_0222 and EX28DRAFT_0223 are separated by 3 nucleotides EX28DRAFT_0223 and EX28DRAFT_0224 are separated by 48 nucleotides EX28DRAFT_0224 and EX28DRAFT_0225 are separated by 258 nucleotides
EX28DRAFT_0220: EX28DRAFT_0220 - Transcriptional regulator, at 174,638 to 175,582
_0220
EX28DRAFT_0221: EX28DRAFT_0221 - FlxA-like protein, at 175,579 to 175,917
_0221
EX28DRAFT_0222: EX28DRAFT_0222 - tRNA, at 176,104 to 176,179
_0222
EX28DRAFT_0223: EX28DRAFT_0223 - tRNA, at 176,183 to 176,258
_0223
EX28DRAFT_0224: EX28DRAFT_0224 - tRNA, at 176,307 to 176,379
_0224
EX28DRAFT_0225: EX28DRAFT_0225 - glutamyl-tRNA synthetase, bacterial family, at 176,638 to 178,053
_0225
Position (kb) 175
176 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 174.584 kb on - strand at 174.660 kb on + strand at 174.660 kb on + strand at 174.673 kb on + strand at 174.673 kb on + strand at 174.980 kb on - strand, within EX28DRAFT_0220 at 175.290 kb on + strand, within EX28DRAFT_0220 at 175.344 kb on + strand, within EX28DRAFT_0220 at 175.498 kb on + strand at 175.580 kb on + strand at 175.580 kb on + strand at 175.581 kb on - strand at 175.581 kb on - strand at 175.586 kb on + strand at 175.618 kb on - strand, within EX28DRAFT_0221 at 175.621 kb on + strand, within EX28DRAFT_0221 at 175.918 kb on - strand at 175.936 kb on - strand at 175.965 kb on - strand at 175.984 kb on + strand at 176.008 kb on - strand at 176.019 kb on - strand at 176.055 kb on - strand at 176.271 kb on + strand at 176.272 kb on - strand at 176.272 kb on - strand at 176.275 kb on + strand at 176.276 kb on - strand at 176.276 kb on - strand at 176.360 kb on - strand, within EX28DRAFT_0224 at 176.444 kb on - strand at 176.444 kb on - strand at 176.457 kb on - strand at 176.619 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 174,584 - -0.2 174,660 + -3.8 174,660 + +1.4 174,673 + -1.3 174,673 + +0.0 174,980 - EX28DRAFT_0220 0.36 +1.7 175,290 + EX28DRAFT_0220 0.69 +0.4 175,344 + EX28DRAFT_0220 0.75 -0.0 175,498 + -2.3 175,580 + +1.2 175,580 + -0.2 175,581 - +0.5 175,581 - -0.1 175,586 + -2.5 175,618 - EX28DRAFT_0221 0.12 -1.1 175,621 + EX28DRAFT_0221 0.12 +0.4 175,918 - +0.2 175,936 - -1.2 175,965 - +0.1 175,984 + -1.3 176,008 - +0.1 176,019 - -0.2 176,055 - +0.5 176,271 + -0.0 176,272 - +0.0 176,272 - -0.6 176,275 + +0.3 176,276 - -0.1 176,276 - +0.7 176,360 - EX28DRAFT_0224 0.73 -0.5 176,444 - +0.2 176,444 - -0.5 176,457 - +0.2 176,619 + -3.3
Or see this region's nucleotide sequence