Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0198 and EX28DRAFT_0199 are separated by 56 nucleotides EX28DRAFT_0199 and EX28DRAFT_0200 overlap by 1 nucleotides EX28DRAFT_0200 and EX28DRAFT_0201 are separated by 212 nucleotides EX28DRAFT_0201 and EX28DRAFT_0202 overlap by 14 nucleotides
EX28DRAFT_0198: EX28DRAFT_0198 - Malic enzyme, at 152,558 to 154,837
_0198
EX28DRAFT_0199: EX28DRAFT_0199 - coproporphyrinogen oxidase (EC 1.3.3.3), at 154,894 to 155,793
_0199
EX28DRAFT_0200: EX28DRAFT_0200 - N-acetylmuramoyl-L-alanine amidase, at 155,793 to 156,668
_0200
EX28DRAFT_0201: EX28DRAFT_0201 - Acetyltransferase (GNAT) family, at 156,881 to 157,306
_0201
EX28DRAFT_0202: EX28DRAFT_0202 - Protein of unknown function (DUF2919), at 157,293 to 157,742
_0202
Position (kb) 155
156
157 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 154.836 kb on - strand at 154.895 kb on + strand at 154.896 kb on - strand at 154.896 kb on - strand at 154.896 kb on - strand at 154.967 kb on + strand at 155.169 kb on - strand, within EX28DRAFT_0199 at 155.391 kb on - strand, within EX28DRAFT_0199 at 155.846 kb on + strand at 156.027 kb on - strand, within EX28DRAFT_0200 at 156.027 kb on - strand, within EX28DRAFT_0200 at 156.041 kb on + strand, within EX28DRAFT_0200 at 156.042 kb on - strand, within EX28DRAFT_0200 at 156.075 kb on - strand, within EX28DRAFT_0200 at 156.109 kb on - strand, within EX28DRAFT_0200 at 156.252 kb on - strand, within EX28DRAFT_0200 at 156.252 kb on - strand, within EX28DRAFT_0200 at 156.275 kb on + strand, within EX28DRAFT_0200 at 156.276 kb on - strand, within EX28DRAFT_0200 at 156.276 kb on - strand, within EX28DRAFT_0200 at 156.319 kb on + strand, within EX28DRAFT_0200 at 156.319 kb on + strand, within EX28DRAFT_0200 at 156.680 kb on - strand at 156.703 kb on + strand at 156.704 kb on - strand at 156.705 kb on + strand at 156.706 kb on - strand at 156.711 kb on - strand at 156.768 kb on - strand at 156.878 kb on - strand at 156.988 kb on + strand, within EX28DRAFT_0201 at 156.988 kb on + strand, within EX28DRAFT_0201 at 156.988 kb on + strand, within EX28DRAFT_0201 at 156.989 kb on - strand, within EX28DRAFT_0201 at 157.088 kb on + strand, within EX28DRAFT_0201 at 157.092 kb on - strand, within EX28DRAFT_0201 at 157.092 kb on - strand, within EX28DRAFT_0201 at 157.232 kb on + strand, within EX28DRAFT_0201 at 157.232 kb on + strand, within EX28DRAFT_0201 at 157.232 kb on + strand, within EX28DRAFT_0201 at 157.233 kb on - strand, within EX28DRAFT_0201 at 157.233 kb on - strand, within EX28DRAFT_0201 at 157.233 kb on - strand, within EX28DRAFT_0201 at 157.233 kb on - strand, within EX28DRAFT_0201 at 157.406 kb on - strand, within EX28DRAFT_0202 at 157.456 kb on - strand, within EX28DRAFT_0202
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 154,836 - +0.8 154,895 + -0.1 154,896 - -0.2 154,896 - -0.0 154,896 - +1.5 154,967 + +1.2 155,169 - EX28DRAFT_0199 0.31 -0.8 155,391 - EX28DRAFT_0199 0.55 -0.4 155,846 + +0.6 156,027 - EX28DRAFT_0200 0.27 +0.7 156,027 - EX28DRAFT_0200 0.27 +0.3 156,041 + EX28DRAFT_0200 0.28 +0.5 156,042 - EX28DRAFT_0200 0.28 +0.7 156,075 - EX28DRAFT_0200 0.32 +0.4 156,109 - EX28DRAFT_0200 0.36 +0.5 156,252 - EX28DRAFT_0200 0.52 +0.4 156,252 - EX28DRAFT_0200 0.52 +0.5 156,275 + EX28DRAFT_0200 0.55 +0.4 156,276 - EX28DRAFT_0200 0.55 -0.4 156,276 - EX28DRAFT_0200 0.55 -2.3 156,319 + EX28DRAFT_0200 0.60 -0.3 156,319 + EX28DRAFT_0200 0.60 +0.2 156,680 - -0.0 156,703 + -1.2 156,704 - -1.3 156,705 + +0.5 156,706 - -2.3 156,711 - +0.3 156,768 - +0.5 156,878 - -0.5 156,988 + EX28DRAFT_0201 0.25 -4.0 156,988 + EX28DRAFT_0201 0.25 +1.6 156,988 + EX28DRAFT_0201 0.25 -0.2 156,989 - EX28DRAFT_0201 0.25 -1.3 157,088 + EX28DRAFT_0201 0.49 -0.5 157,092 - EX28DRAFT_0201 0.50 -3.6 157,092 - EX28DRAFT_0201 0.50 +2.7 157,232 + EX28DRAFT_0201 0.82 -0.2 157,232 + EX28DRAFT_0201 0.82 +0.3 157,232 + EX28DRAFT_0201 0.82 +1.1 157,233 - EX28DRAFT_0201 0.83 -0.2 157,233 - EX28DRAFT_0201 0.83 -0.4 157,233 - EX28DRAFT_0201 0.83 -3.1 157,233 - EX28DRAFT_0201 0.83 -1.5 157,406 - EX28DRAFT_0202 0.25 +0.8 157,456 - EX28DRAFT_0202 0.36 -0.9
Or see this region's nucleotide sequence