Experiment: 30_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS15295 and AMB_RS15300 are separated by 122 nucleotides AMB_RS15300 and AMB_RS15305 are separated by 223 nucleotides AMB_RS15305 and AMB_RS15310 are separated by 13 nucleotides
AMB_RS15295: AMB_RS15295 - iron donor protein CyaY, at 3,277,724 to 3,278,029
_RS15295
AMB_RS15300: AMB_RS15300 - 50S ribosomal protein L31, at 3,278,152 to 3,278,385
_RS15300
AMB_RS15305: AMB_RS15305 - adenylate/guanylate cyclase domain-containing protein, at 3,278,609 to 3,280,360
_RS15305
AMB_RS15310: AMB_RS15310 - adenylate/guanylate cyclase domain-containing protein, at 3,280,374 to 3,282,020
_RS15310
Position (kb)
3278
3279
3280
3281 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3277.884 kb on + strand, within AMB_RS15295 at 3277.884 kb on + strand, within AMB_RS15295 at 3277.884 kb on + strand, within AMB_RS15295 at 3277.884 kb on + strand, within AMB_RS15295 at 3277.885 kb on - strand, within AMB_RS15295 at 3277.885 kb on - strand, within AMB_RS15295 at 3278.121 kb on + strand at 3278.121 kb on + strand at 3278.121 kb on + strand at 3278.121 kb on + strand at 3278.517 kb on - strand at 3278.529 kb on + strand at 3278.532 kb on + strand at 3278.533 kb on - strand at 3278.564 kb on + strand at 3278.564 kb on + strand at 3278.565 kb on - strand at 3278.633 kb on + strand at 3278.762 kb on + strand at 3278.762 kb on + strand at 3278.763 kb on - strand at 3278.763 kb on - strand at 3278.801 kb on + strand, within AMB_RS15305 at 3278.802 kb on - strand, within AMB_RS15305 at 3279.083 kb on + strand, within AMB_RS15305 at 3279.084 kb on - strand, within AMB_RS15305 at 3279.084 kb on - strand, within AMB_RS15305 at 3279.217 kb on + strand, within AMB_RS15305 at 3279.217 kb on + strand, within AMB_RS15305 at 3279.548 kb on + strand, within AMB_RS15305 at 3279.548 kb on + strand, within AMB_RS15305 at 3279.549 kb on - strand, within AMB_RS15305 at 3279.695 kb on + strand, within AMB_RS15305 at 3279.696 kb on - strand, within AMB_RS15305 at 3279.696 kb on - strand, within AMB_RS15305 at 3279.713 kb on + strand, within AMB_RS15305 at 3279.713 kb on + strand, within AMB_RS15305 at 3279.714 kb on - strand, within AMB_RS15305 at 3279.731 kb on + strand, within AMB_RS15305 at 3279.835 kb on + strand, within AMB_RS15305 at 3279.835 kb on + strand, within AMB_RS15305 at 3279.836 kb on - strand, within AMB_RS15305 at 3280.012 kb on + strand, within AMB_RS15305 at 3280.376 kb on - strand at 3280.804 kb on + strand, within AMB_RS15310 at 3280.804 kb on + strand, within AMB_RS15310 at 3280.876 kb on + strand, within AMB_RS15310 at 3280.876 kb on + strand, within AMB_RS15310 at 3280.877 kb on - strand, within AMB_RS15310
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_2 remove 3,277,884 + AMB_RS15295 0.52 +0.7 3,277,884 + AMB_RS15295 0.52 -1.9 3,277,884 + AMB_RS15295 0.52 -0.2 3,277,884 + AMB_RS15295 0.52 -3.7 3,277,885 - AMB_RS15295 0.53 -2.2 3,277,885 - AMB_RS15295 0.53 -1.5 3,278,121 + -0.0 3,278,121 + -2.1 3,278,121 + -0.6 3,278,121 + -0.5 3,278,517 - -0.3 3,278,529 + +0.1 3,278,532 + -0.5 3,278,533 - -0.1 3,278,564 + -0.6 3,278,564 + +0.8 3,278,565 - -0.8 3,278,633 + +0.0 3,278,762 + -0.5 3,278,762 + +2.7 3,278,763 - +0.3 3,278,763 - -0.8 3,278,801 + AMB_RS15305 0.11 -1.2 3,278,802 - AMB_RS15305 0.11 -1.5 3,279,083 + AMB_RS15305 0.27 -0.1 3,279,084 - AMB_RS15305 0.27 -0.2 3,279,084 - AMB_RS15305 0.27 -1.9 3,279,217 + AMB_RS15305 0.35 +0.5 3,279,217 + AMB_RS15305 0.35 -1.5 3,279,548 + AMB_RS15305 0.54 -0.2 3,279,548 + AMB_RS15305 0.54 -1.1 3,279,549 - AMB_RS15305 0.54 -0.3 3,279,695 + AMB_RS15305 0.62 -0.1 3,279,696 - AMB_RS15305 0.62 -0.4 3,279,696 - AMB_RS15305 0.62 +0.2 3,279,713 + AMB_RS15305 0.63 +0.1 3,279,713 + AMB_RS15305 0.63 -0.1 3,279,714 - AMB_RS15305 0.63 -3.0 3,279,731 + AMB_RS15305 0.64 -0.4 3,279,835 + AMB_RS15305 0.70 +0.6 3,279,835 + AMB_RS15305 0.70 -0.3 3,279,836 - AMB_RS15305 0.70 +0.3 3,280,012 + AMB_RS15305 0.80 +0.1 3,280,376 - +0.0 3,280,804 + AMB_RS15310 0.26 -2.1 3,280,804 + AMB_RS15310 0.26 -0.5 3,280,876 + AMB_RS15310 0.30 -0.7 3,280,876 + AMB_RS15310 0.30 -0.1 3,280,877 - AMB_RS15310 0.31 -1.2
Or see this region's nucleotide sequence