Experiment: 30_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12465 and AMB_RS12470 are separated by 201 nucleotides AMB_RS12470 and AMB_RS12475 are separated by 42 nucleotides AMB_RS12475 and AMB_RS12480 are separated by 34 nucleotides AMB_RS12480 and AMB_RS12485 overlap by 8 nucleotides
AMB_RS12465: AMB_RS12465 - DUF4170 domain-containing protein, at 2,658,951 to 2,659,358
_RS12465
AMB_RS12470: AMB_RS12470 - hypothetical protein, at 2,659,560 to 2,659,826
_RS12470
AMB_RS12475: AMB_RS12475 - FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO, at 2,659,869 to 2,661,212
_RS12475
AMB_RS12480: AMB_RS12480 - lactoylglutathione lyase, at 2,661,247 to 2,661,639
_RS12480
AMB_RS12485: AMB_RS12485 - lipid-A-disaccharide synthase, at 2,661,632 to 2,662,804
_RS12485
Position (kb)
2659
2660
2661
2662 Strain fitness (log2 ratio)
-2
-1
0
1 at 2658.920 kb on + strand at 2658.939 kb on + strand at 2659.403 kb on - strand at 2659.559 kb on + strand at 2659.560 kb on - strand at 2659.560 kb on - strand at 2659.560 kb on - strand at 2659.560 kb on - strand at 2659.665 kb on + strand, within AMB_RS12470 at 2659.665 kb on + strand, within AMB_RS12470 at 2659.665 kb on + strand, within AMB_RS12470 at 2659.666 kb on - strand, within AMB_RS12470 at 2659.666 kb on - strand, within AMB_RS12470 at 2659.856 kb on + strand at 2659.857 kb on - strand at 2660.269 kb on + strand, within AMB_RS12475 at 2660.270 kb on - strand, within AMB_RS12475 at 2660.270 kb on - strand, within AMB_RS12475 at 2660.445 kb on + strand, within AMB_RS12475 at 2660.476 kb on - strand, within AMB_RS12475 at 2660.476 kb on - strand, within AMB_RS12475 at 2660.479 kb on - strand, within AMB_RS12475 at 2660.479 kb on - strand, within AMB_RS12475 at 2660.479 kb on - strand, within AMB_RS12475 at 2660.479 kb on - strand, within AMB_RS12475 at 2660.479 kb on - strand, within AMB_RS12475 at 2660.575 kb on + strand, within AMB_RS12475 at 2660.575 kb on + strand, within AMB_RS12475 at 2660.576 kb on - strand, within AMB_RS12475 at 2660.608 kb on - strand, within AMB_RS12475 at 2660.703 kb on + strand, within AMB_RS12475 at 2660.703 kb on + strand, within AMB_RS12475 at 2660.704 kb on - strand, within AMB_RS12475 at 2660.916 kb on + strand, within AMB_RS12475 at 2660.917 kb on - strand, within AMB_RS12475 at 2660.917 kb on - strand, within AMB_RS12475 at 2660.917 kb on - strand, within AMB_RS12475 at 2661.060 kb on + strand, within AMB_RS12475 at 2661.060 kb on + strand, within AMB_RS12475 at 2661.061 kb on - strand, within AMB_RS12475 at 2661.156 kb on + strand at 2661.156 kb on + strand at 2661.157 kb on - strand at 2661.273 kb on + strand at 2661.274 kb on - strand at 2661.275 kb on + strand at 2661.276 kb on - strand at 2661.383 kb on + strand, within AMB_RS12480 at 2661.383 kb on + strand, within AMB_RS12480 at 2661.383 kb on + strand, within AMB_RS12480 at 2661.383 kb on + strand, within AMB_RS12480 at 2661.384 kb on - strand, within AMB_RS12480 at 2661.384 kb on - strand, within AMB_RS12480 at 2661.384 kb on - strand, within AMB_RS12480 at 2661.384 kb on - strand, within AMB_RS12480 at 2661.434 kb on + strand, within AMB_RS12480 at 2661.572 kb on + strand, within AMB_RS12480 at 2661.573 kb on - strand, within AMB_RS12480 at 2661.573 kb on - strand, within AMB_RS12480 at 2661.573 kb on - strand, within AMB_RS12480
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_2 remove 2,658,920 + -0.1 2,658,939 + +0.7 2,659,403 - -1.1 2,659,559 + -2.0 2,659,560 - +0.2 2,659,560 - -1.0 2,659,560 - +0.2 2,659,560 - -0.6 2,659,665 + AMB_RS12470 0.39 -2.4 2,659,665 + AMB_RS12470 0.39 -0.8 2,659,665 + AMB_RS12470 0.39 +0.2 2,659,666 - AMB_RS12470 0.40 -0.9 2,659,666 - AMB_RS12470 0.40 -1.2 2,659,856 + -1.4 2,659,857 - -0.3 2,660,269 + AMB_RS12475 0.30 +0.1 2,660,270 - AMB_RS12475 0.30 -0.6 2,660,270 - AMB_RS12475 0.30 -0.0 2,660,445 + AMB_RS12475 0.43 -0.0 2,660,476 - AMB_RS12475 0.45 -0.9 2,660,476 - AMB_RS12475 0.45 +0.1 2,660,479 - AMB_RS12475 0.45 +0.6 2,660,479 - AMB_RS12475 0.45 +0.2 2,660,479 - AMB_RS12475 0.45 -0.1 2,660,479 - AMB_RS12475 0.45 -0.8 2,660,479 - AMB_RS12475 0.45 +1.6 2,660,575 + AMB_RS12475 0.53 -0.6 2,660,575 + AMB_RS12475 0.53 -1.0 2,660,576 - AMB_RS12475 0.53 +0.6 2,660,608 - AMB_RS12475 0.55 +0.0 2,660,703 + AMB_RS12475 0.62 -1.4 2,660,703 + AMB_RS12475 0.62 -0.5 2,660,704 - AMB_RS12475 0.62 -0.5 2,660,916 + AMB_RS12475 0.78 -0.6 2,660,917 - AMB_RS12475 0.78 -0.3 2,660,917 - AMB_RS12475 0.78 -0.9 2,660,917 - AMB_RS12475 0.78 -0.1 2,661,060 + AMB_RS12475 0.89 -0.4 2,661,060 + AMB_RS12475 0.89 -0.4 2,661,061 - AMB_RS12475 0.89 -0.3 2,661,156 + -0.2 2,661,156 + -0.2 2,661,157 - -0.1 2,661,273 + +0.4 2,661,274 - -0.1 2,661,275 + -0.2 2,661,276 - -0.4 2,661,383 + AMB_RS12480 0.35 -1.3 2,661,383 + AMB_RS12480 0.35 -0.6 2,661,383 + AMB_RS12480 0.35 +0.9 2,661,383 + AMB_RS12480 0.35 -1.4 2,661,384 - AMB_RS12480 0.35 +0.2 2,661,384 - AMB_RS12480 0.35 -0.3 2,661,384 - AMB_RS12480 0.35 -0.4 2,661,384 - AMB_RS12480 0.35 +0.3 2,661,434 + AMB_RS12480 0.48 -0.9 2,661,572 + AMB_RS12480 0.83 +0.3 2,661,573 - AMB_RS12480 0.83 -0.7 2,661,573 - AMB_RS12480 0.83 -0.2 2,661,573 - AMB_RS12480 0.83 +1.1
Or see this region's nucleotide sequence