Experiment: 30_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10655 and AMB_RS10660 are separated by 80 nucleotides AMB_RS10660 and AMB_RS10665 are separated by 81 nucleotides AMB_RS10665 and AMB_RS10670 overlap by 4 nucleotides
AMB_RS10655: AMB_RS10655 - glucokinase, at 2,286,735 to 2,287,700
_RS10655
AMB_RS10660: AMB_RS10660 - hypothetical protein, at 2,287,781 to 2,288,320
_RS10660
AMB_RS10665: AMB_RS10665 - phosphoribosylformylglycinamidine synthase subunit PurQ, at 2,288,402 to 2,289,091
_RS10665
AMB_RS10670: AMB_RS10670 - phosphoribosylformylglycinamidine synthase II, at 2,289,088 to 2,291,293
_RS10670
Position (kb)
2287
2288
2289 Strain fitness (log2 ratio)
-1
0
1
2 at 2286.874 kb on - strand, within AMB_RS10655 at 2286.874 kb on - strand, within AMB_RS10655 at 2286.874 kb on - strand, within AMB_RS10655 at 2287.335 kb on + strand, within AMB_RS10655 at 2287.335 kb on + strand, within AMB_RS10655 at 2287.335 kb on + strand, within AMB_RS10655 at 2287.336 kb on - strand, within AMB_RS10655 at 2287.513 kb on + strand, within AMB_RS10655 at 2287.513 kb on + strand, within AMB_RS10655 at 2287.513 kb on + strand, within AMB_RS10655 at 2287.513 kb on + strand, within AMB_RS10655 at 2287.513 kb on + strand, within AMB_RS10655 at 2287.514 kb on - strand, within AMB_RS10655 at 2287.514 kb on - strand, within AMB_RS10655 at 2287.571 kb on + strand, within AMB_RS10655 at 2287.608 kb on + strand at 2287.688 kb on + strand at 2287.688 kb on + strand at 2287.698 kb on + strand at 2287.698 kb on + strand at 2287.699 kb on - strand at 2288.000 kb on - strand, within AMB_RS10660 at 2288.047 kb on + strand, within AMB_RS10660 at 2288.243 kb on + strand, within AMB_RS10660 at 2288.243 kb on + strand, within AMB_RS10660 at 2288.244 kb on - strand, within AMB_RS10660
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_2 remove 2,286,874 - AMB_RS10655 0.14 +0.5 2,286,874 - AMB_RS10655 0.14 -0.2 2,286,874 - AMB_RS10655 0.14 -0.6 2,287,335 + AMB_RS10655 0.62 -0.4 2,287,335 + AMB_RS10655 0.62 -0.4 2,287,335 + AMB_RS10655 0.62 -1.5 2,287,336 - AMB_RS10655 0.62 +0.2 2,287,513 + AMB_RS10655 0.81 -0.8 2,287,513 + AMB_RS10655 0.81 -0.7 2,287,513 + AMB_RS10655 0.81 -0.4 2,287,513 + AMB_RS10655 0.81 -1.6 2,287,513 + AMB_RS10655 0.81 -0.0 2,287,514 - AMB_RS10655 0.81 +0.0 2,287,514 - AMB_RS10655 0.81 -0.3 2,287,571 + AMB_RS10655 0.87 -0.1 2,287,608 + +1.9 2,287,688 + +1.4 2,287,688 + -1.7 2,287,698 + +1.0 2,287,698 + -0.1 2,287,699 - -1.2 2,288,000 - AMB_RS10660 0.41 -0.8 2,288,047 + AMB_RS10660 0.49 +0.3 2,288,243 + AMB_RS10660 0.86 -0.1 2,288,243 + AMB_RS10660 0.86 -1.0 2,288,244 - AMB_RS10660 0.86 +0.7
Or see this region's nucleotide sequence