Experiment: 30_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS09325 and AMB_RS09330 are separated by 131 nucleotides AMB_RS09330 and AMB_RS09335 overlap by 4 nucleotides AMB_RS09335 and AMB_RS09340 are separated by 361 nucleotides
AMB_RS09325: AMB_RS09325 - cell envelope integrity protein CreD, at 1,984,678 to 1,986,042
_RS09325
AMB_RS09330: AMB_RS09330 - DNA-binding response regulator, at 1,986,174 to 1,986,863
_RS09330
AMB_RS09335: AMB_RS09335 - HAMP domain-containing protein, at 1,986,860 to 1,988,437
_RS09335
AMB_RS09340: AMB_RS09340 - CBS domain-containing protein, at 1,988,799 to 1,990,166
_RS09340
Position (kb)
1986
1987
1988
1989 Strain fitness (log2 ratio)
-1
0
1
2 at 1986.209 kb on + strand at 1986.465 kb on + strand, within AMB_RS09330 at 1986.465 kb on + strand, within AMB_RS09330 at 1986.466 kb on - strand, within AMB_RS09330 at 1986.741 kb on + strand, within AMB_RS09330 at 1986.741 kb on + strand, within AMB_RS09330 at 1986.741 kb on + strand, within AMB_RS09330 at 1986.741 kb on + strand, within AMB_RS09330 at 1986.741 kb on + strand, within AMB_RS09330 at 1986.741 kb on + strand, within AMB_RS09330 at 1986.742 kb on - strand, within AMB_RS09330 at 1986.840 kb on + strand at 1986.840 kb on + strand at 1986.841 kb on - strand at 1986.953 kb on + strand at 1987.106 kb on + strand, within AMB_RS09335 at 1987.107 kb on - strand, within AMB_RS09335 at 1987.107 kb on - strand, within AMB_RS09335 at 1987.800 kb on - strand, within AMB_RS09335 at 1987.800 kb on - strand, within AMB_RS09335 at 1987.858 kb on + strand, within AMB_RS09335 at 1988.338 kb on - strand at 1988.435 kb on + strand at 1988.435 kb on + strand at 1988.436 kb on - strand at 1988.542 kb on - strand at 1988.590 kb on + strand at 1988.591 kb on - strand at 1988.591 kb on - strand at 1988.591 kb on - strand at 1988.591 kb on - strand at 1988.591 kb on - strand at 1988.591 kb on - strand at 1988.762 kb on + strand at 1988.763 kb on - strand at 1988.763 kb on - strand at 1988.763 kb on - strand at 1988.768 kb on + strand at 1988.780 kb on + strand at 1988.786 kb on + strand at 1988.786 kb on + strand at 1988.786 kb on + strand at 1988.787 kb on - strand at 1988.838 kb on - strand at 1988.855 kb on + strand at 1988.855 kb on + strand at 1988.856 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_2 remove 1,986,209 + -0.2 1,986,465 + AMB_RS09330 0.42 -0.1 1,986,465 + AMB_RS09330 0.42 +0.2 1,986,466 - AMB_RS09330 0.42 -0.1 1,986,741 + AMB_RS09330 0.82 -0.1 1,986,741 + AMB_RS09330 0.82 -0.3 1,986,741 + AMB_RS09330 0.82 -0.3 1,986,741 + AMB_RS09330 0.82 +0.2 1,986,741 + AMB_RS09330 0.82 -0.5 1,986,741 + AMB_RS09330 0.82 -0.4 1,986,742 - AMB_RS09330 0.82 +0.0 1,986,840 + -0.8 1,986,840 + -0.4 1,986,841 - -0.2 1,986,953 + -1.5 1,987,106 + AMB_RS09335 0.16 -0.2 1,987,107 - AMB_RS09335 0.16 -0.8 1,987,107 - AMB_RS09335 0.16 +0.0 1,987,800 - AMB_RS09335 0.60 +1.0 1,987,800 - AMB_RS09335 0.60 +0.1 1,987,858 + AMB_RS09335 0.63 +0.1 1,988,338 - +1.1 1,988,435 + +2.1 1,988,435 + -0.7 1,988,436 - -0.3 1,988,542 - +0.1 1,988,590 + +0.2 1,988,591 - -1.2 1,988,591 - -0.2 1,988,591 - +1.2 1,988,591 - -1.2 1,988,591 - -0.4 1,988,591 - +0.5 1,988,762 + -0.4 1,988,763 - -0.6 1,988,763 - -0.1 1,988,763 - +0.1 1,988,768 + -0.5 1,988,780 + -0.9 1,988,786 + -0.4 1,988,786 + -0.2 1,988,786 + -0.0 1,988,787 - -0.5 1,988,838 - -1.5 1,988,855 + +0.5 1,988,855 + -0.0 1,988,856 - -1.0
Or see this region's nucleotide sequence