Experiment: 30_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23920 and AMB_RS04720 are separated by 305 nucleotides AMB_RS04720 and AMB_RS04725 are separated by 85 nucleotides AMB_RS04725 and AMB_RS04735 are separated by 325 nucleotides
AMB_RS23920: AMB_RS23920 - transcriptional repressor, at 986,144 to 986,539
_RS23920
AMB_RS04720: AMB_RS04720 - response regulator, at 986,845 to 987,225
_RS04720
AMB_RS04725: AMB_RS04725 - transcriptional regulator NrdR, at 987,311 to 987,772
_RS04725
AMB_RS04735: AMB_RS04735 - diguanylate cyclase, at 988,098 to 989,627
_RS04735
Position (kb) 987
988 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 986.431 kb on + strand, within AMB_RS23920 at 986.432 kb on - strand, within AMB_RS23920 at 986.432 kb on - strand, within AMB_RS23920 at 986.432 kb on - strand, within AMB_RS23920 at 986.659 kb on + strand at 986.713 kb on + strand at 986.714 kb on - strand at 986.803 kb on + strand at 986.808 kb on - strand at 986.939 kb on - strand, within AMB_RS04720 at 987.044 kb on + strand, within AMB_RS04720 at 987.060 kb on - strand, within AMB_RS04720 at 987.060 kb on - strand, within AMB_RS04720 at 987.060 kb on - strand, within AMB_RS04720 at 987.094 kb on - strand, within AMB_RS04720 at 987.164 kb on - strand, within AMB_RS04720 at 987.223 kb on + strand at 987.227 kb on + strand at 987.227 kb on + strand at 987.228 kb on - strand at 987.228 kb on - strand at 987.264 kb on + strand at 987.283 kb on + strand at 987.283 kb on + strand at 987.283 kb on + strand at 987.290 kb on + strand at 987.290 kb on + strand at 987.291 kb on - strand at 987.291 kb on - strand at 987.312 kb on + strand at 987.312 kb on + strand at 987.423 kb on - strand, within AMB_RS04725 at 987.423 kb on - strand, within AMB_RS04725 at 987.571 kb on + strand, within AMB_RS04725 at 987.571 kb on + strand, within AMB_RS04725 at 987.572 kb on - strand, within AMB_RS04725 at 987.572 kb on - strand, within AMB_RS04725 at 987.572 kb on - strand, within AMB_RS04725 at 987.572 kb on - strand, within AMB_RS04725 at 987.772 kb on + strand at 987.773 kb on - strand at 987.773 kb on - strand at 987.773 kb on - strand at 987.872 kb on + strand at 987.872 kb on + strand at 987.872 kb on + strand at 987.873 kb on - strand at 987.918 kb on + strand at 987.918 kb on + strand at 987.918 kb on + strand at 987.919 kb on - strand at 987.922 kb on + strand at 987.923 kb on - strand at 987.923 kb on - strand at 987.960 kb on + strand at 987.961 kb on - strand at 988.003 kb on + strand at 988.003 kb on + strand at 988.003 kb on + strand at 988.004 kb on - strand at 988.083 kb on - strand at 988.206 kb on + strand at 988.206 kb on + strand at 988.206 kb on + strand at 988.206 kb on + strand at 988.206 kb on + strand at 988.207 kb on - strand at 988.345 kb on + strand, within AMB_RS04735 at 988.345 kb on + strand, within AMB_RS04735 at 988.345 kb on + strand, within AMB_RS04735 at 988.346 kb on - strand, within AMB_RS04735 at 988.346 kb on - strand, within AMB_RS04735 at 988.346 kb on - strand, within AMB_RS04735 at 988.393 kb on - strand, within AMB_RS04735 at 988.393 kb on - strand, within AMB_RS04735 at 988.432 kb on + strand, within AMB_RS04735 at 988.432 kb on + strand, within AMB_RS04735 at 988.433 kb on - strand, within AMB_RS04735 at 988.433 kb on - strand, within AMB_RS04735 at 988.433 kb on - strand, within AMB_RS04735 at 988.433 kb on - strand, within AMB_RS04735 at 988.490 kb on + strand, within AMB_RS04735 at 988.491 kb on - strand, within AMB_RS04735 at 988.491 kb on - strand, within AMB_RS04735 at 988.771 kb on - strand, within AMB_RS04735 at 988.771 kb on - strand, within AMB_RS04735
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_2 remove 986,431 + AMB_RS23920 0.72 +0.3 986,432 - AMB_RS23920 0.73 +0.1 986,432 - AMB_RS23920 0.73 +0.0 986,432 - AMB_RS23920 0.73 -0.5 986,659 + -0.6 986,713 + +0.2 986,714 - -1.2 986,803 + -0.2 986,808 - +0.3 986,939 - AMB_RS04720 0.25 +0.1 987,044 + AMB_RS04720 0.52 -0.1 987,060 - AMB_RS04720 0.56 +0.5 987,060 - AMB_RS04720 0.56 +0.1 987,060 - AMB_RS04720 0.56 +0.7 987,094 - AMB_RS04720 0.65 -0.0 987,164 - AMB_RS04720 0.84 +1.4 987,223 + -0.3 987,227 + -1.1 987,227 + +1.6 987,228 - -0.4 987,228 - +0.1 987,264 + -0.9 987,283 + +0.1 987,283 + +0.3 987,283 + +0.5 987,290 + -0.5 987,290 + -0.2 987,291 - +0.1 987,291 - -0.6 987,312 + +0.1 987,312 + -0.3 987,423 - AMB_RS04725 0.24 -0.7 987,423 - AMB_RS04725 0.24 +0.1 987,571 + AMB_RS04725 0.56 +1.1 987,571 + AMB_RS04725 0.56 +0.1 987,572 - AMB_RS04725 0.56 -0.1 987,572 - AMB_RS04725 0.56 -0.9 987,572 - AMB_RS04725 0.56 +1.1 987,572 - AMB_RS04725 0.56 +0.4 987,772 + -2.8 987,773 - -0.0 987,773 - -1.3 987,773 - -0.0 987,872 + -0.4 987,872 + -0.6 987,872 + +0.3 987,873 - -0.2 987,918 + +0.2 987,918 + -0.6 987,918 + -0.2 987,919 - -0.2 987,922 + -0.0 987,923 - -0.6 987,923 - -0.7 987,960 + -0.4 987,961 - +0.1 988,003 + +1.5 988,003 + -0.4 988,003 + -0.2 988,004 - -0.2 988,083 - +0.2 988,206 + -0.6 988,206 + +0.5 988,206 + -0.0 988,206 + -0.9 988,206 + +0.9 988,207 - -0.5 988,345 + AMB_RS04735 0.16 -1.0 988,345 + AMB_RS04735 0.16 -0.8 988,345 + AMB_RS04735 0.16 -0.7 988,346 - AMB_RS04735 0.16 -0.5 988,346 - AMB_RS04735 0.16 -0.4 988,346 - AMB_RS04735 0.16 -0.4 988,393 - AMB_RS04735 0.19 -0.2 988,393 - AMB_RS04735 0.19 -0.7 988,432 + AMB_RS04735 0.22 -0.3 988,432 + AMB_RS04735 0.22 -0.9 988,433 - AMB_RS04735 0.22 +0.4 988,433 - AMB_RS04735 0.22 -1.3 988,433 - AMB_RS04735 0.22 -0.2 988,433 - AMB_RS04735 0.22 +0.8 988,490 + AMB_RS04735 0.26 -1.1 988,491 - AMB_RS04735 0.26 -0.2 988,491 - AMB_RS04735 0.26 -1.2 988,771 - AMB_RS04735 0.44 +0.9 988,771 - AMB_RS04735 0.44 +2.6
Or see this region's nucleotide sequence