Experiment: 30_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04475 and AMB_RS04480 are separated by 2 nucleotides AMB_RS04480 and AMB_RS04485 are separated by 60 nucleotides AMB_RS04485 and AMB_RS04490 are separated by 213 nucleotides AMB_RS04490 and AMB_RS04495 are separated by 23 nucleotides
AMB_RS04475: AMB_RS04475 - S41 family peptidase, at 938,874 to 940,430
_RS04475
AMB_RS04480: AMB_RS04480 - acyl-CoA dehydrogenase, at 940,433 to 941,263
_RS04480
AMB_RS04485: AMB_RS04485 - NAD(P)-dependent oxidoreductase, at 941,324 to 942,076
_RS04485
AMB_RS04490: AMB_RS04490 - CBS domain-containing protein, at 942,290 to 942,682
_RS04490
AMB_RS04495: AMB_RS04495 - cytochrome c-552 precursor, at 942,706 to 943,626
_RS04495
Position (kb) 941
942
943 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 940.344 kb on + strand at 940.577 kb on + strand, within AMB_RS04480 at 940.577 kb on + strand, within AMB_RS04480 at 940.578 kb on - strand, within AMB_RS04480 at 941.013 kb on - strand, within AMB_RS04480 at 941.125 kb on + strand, within AMB_RS04480 at 941.125 kb on + strand, within AMB_RS04480 at 941.126 kb on - strand, within AMB_RS04480 at 941.261 kb on + strand at 941.261 kb on + strand at 941.262 kb on - strand at 941.262 kb on - strand at 941.262 kb on - strand at 941.264 kb on + strand at 941.264 kb on + strand at 941.265 kb on - strand at 941.371 kb on + strand at 941.371 kb on + strand at 941.371 kb on + strand at 941.372 kb on - strand at 941.372 kb on - strand at 941.372 kb on - strand at 941.625 kb on + strand, within AMB_RS04485 at 941.625 kb on + strand, within AMB_RS04485 at 941.625 kb on + strand, within AMB_RS04485 at 941.626 kb on - strand, within AMB_RS04485 at 941.626 kb on - strand, within AMB_RS04485 at 942.083 kb on + strand at 942.084 kb on - strand at 942.085 kb on + strand at 942.085 kb on + strand at 942.085 kb on + strand at 942.086 kb on - strand at 942.203 kb on + strand at 942.217 kb on + strand at 942.249 kb on + strand at 942.249 kb on + strand at 942.264 kb on + strand at 942.264 kb on + strand at 942.265 kb on - strand at 942.265 kb on - strand at 942.265 kb on - strand at 942.265 kb on - strand at 942.265 kb on - strand at 942.266 kb on + strand at 942.266 kb on + strand at 942.266 kb on + strand at 942.266 kb on + strand at 942.266 kb on + strand at 942.266 kb on + strand at 942.267 kb on - strand at 942.267 kb on - strand at 942.267 kb on - strand at 942.267 kb on - strand at 942.267 kb on - strand at 942.317 kb on + strand at 942.317 kb on + strand at 942.317 kb on + strand at 942.317 kb on + strand at 942.318 kb on - strand at 942.318 kb on - strand at 942.318 kb on - strand at 942.318 kb on - strand at 942.320 kb on - strand at 942.320 kb on - strand at 942.320 kb on - strand at 942.320 kb on - strand at 942.394 kb on + strand, within AMB_RS04490 at 942.490 kb on + strand, within AMB_RS04490 at 942.601 kb on + strand, within AMB_RS04490 at 942.601 kb on + strand, within AMB_RS04490 at 942.602 kb on - strand, within AMB_RS04490 at 942.707 kb on + strand at 942.707 kb on + strand at 942.707 kb on + strand at 942.707 kb on + strand at 942.707 kb on + strand at 942.708 kb on - strand at 942.708 kb on - strand at 942.708 kb on - strand at 942.708 kb on - strand at 942.708 kb on - strand at 942.773 kb on + strand at 942.773 kb on + strand at 942.773 kb on + strand at 942.773 kb on + strand at 942.773 kb on + strand at 942.773 kb on + strand at 942.774 kb on - strand at 942.774 kb on - strand at 942.774 kb on - strand at 942.774 kb on - strand at 942.774 kb on - strand at 942.824 kb on + strand, within AMB_RS04495 at 942.824 kb on + strand, within AMB_RS04495 at 942.824 kb on + strand, within AMB_RS04495 at 942.824 kb on + strand, within AMB_RS04495 at 942.825 kb on - strand, within AMB_RS04495 at 943.017 kb on - strand, within AMB_RS04495 at 943.017 kb on - strand, within AMB_RS04495
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_2 remove 940,344 + -1.2 940,577 + AMB_RS04480 0.17 -0.4 940,577 + AMB_RS04480 0.17 -1.2 940,578 - AMB_RS04480 0.17 +0.2 941,013 - AMB_RS04480 0.70 -1.6 941,125 + AMB_RS04480 0.83 -0.4 941,125 + AMB_RS04480 0.83 +0.5 941,126 - AMB_RS04480 0.83 -0.5 941,261 + -0.0 941,261 + -0.9 941,262 - -0.2 941,262 - +0.3 941,262 - -0.7 941,264 + +0.8 941,264 + +0.8 941,265 - -0.1 941,371 + -0.2 941,371 + -0.4 941,371 + -0.4 941,372 - -3.4 941,372 - -0.9 941,372 - +0.2 941,625 + AMB_RS04485 0.40 +0.3 941,625 + AMB_RS04485 0.40 -0.0 941,625 + AMB_RS04485 0.40 -0.4 941,626 - AMB_RS04485 0.40 -0.8 941,626 - AMB_RS04485 0.40 -0.6 942,083 + -0.7 942,084 - -0.8 942,085 + -0.6 942,085 + +0.1 942,085 + -0.1 942,086 - -0.1 942,203 + -0.3 942,217 + -0.7 942,249 + -0.2 942,249 + +0.1 942,264 + +1.6 942,264 + -0.0 942,265 - -1.1 942,265 - +1.1 942,265 - +0.1 942,265 - -0.4 942,265 - -0.6 942,266 + -0.4 942,266 + +1.4 942,266 + +0.6 942,266 + +2.7 942,266 + -0.0 942,266 + -0.3 942,267 - -0.9 942,267 - -0.4 942,267 - -0.7 942,267 - +0.1 942,267 - +0.0 942,317 + +0.0 942,317 + -0.4 942,317 + -0.2 942,317 + -0.1 942,318 - +0.3 942,318 - -0.2 942,318 - -0.5 942,318 - -2.7 942,320 - -0.2 942,320 - -0.3 942,320 - -0.5 942,320 - -0.7 942,394 + AMB_RS04490 0.26 +0.1 942,490 + AMB_RS04490 0.51 +1.1 942,601 + AMB_RS04490 0.79 -0.4 942,601 + AMB_RS04490 0.79 +0.7 942,602 - AMB_RS04490 0.79 -0.9 942,707 + +0.5 942,707 + +0.1 942,707 + +0.9 942,707 + -1.3 942,707 + +0.3 942,708 - -0.2 942,708 - -0.2 942,708 - -0.1 942,708 - -0.3 942,708 - +0.8 942,773 + -0.3 942,773 + +0.7 942,773 + -1.3 942,773 + -0.3 942,773 + -0.9 942,773 + +0.7 942,774 - -0.4 942,774 - +0.0 942,774 - +0.0 942,774 - +0.5 942,774 - -0.3 942,824 + AMB_RS04495 0.13 -0.5 942,824 + AMB_RS04495 0.13 -2.7 942,824 + AMB_RS04495 0.13 +0.8 942,824 + AMB_RS04495 0.13 -1.9 942,825 - AMB_RS04495 0.13 -0.4 943,017 - AMB_RS04495 0.34 -0.7 943,017 - AMB_RS04495 0.34 +0.7
Or see this region's nucleotide sequence