Experiment: 30_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03205 and AMB_RS03210 are separated by 435 nucleotides AMB_RS03210 and AMB_RS03215 are separated by 102 nucleotides
AMB_RS03205: AMB_RS03205 - DNA recombination/repair protein RecA, at 668,296 to 669,375
_RS03205
AMB_RS03210: AMB_RS03210 - DNA polymerase IV, at 669,811 to 670,992
_RS03210
AMB_RS03215: AMB_RS03215 - alpha/beta hydrolase, at 671,095 to 672,141
_RS03215
Position (kb) 669
670
671 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 669.119 kb on - strand, within AMB_RS03205 at 669.205 kb on + strand, within AMB_RS03205 at 669.205 kb on + strand, within AMB_RS03205 at 669.206 kb on - strand, within AMB_RS03205 at 669.373 kb on + strand at 669.374 kb on - strand at 669.380 kb on + strand at 669.513 kb on + strand at 669.513 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.791 kb on + strand at 669.799 kb on + strand at 669.800 kb on - strand at 669.800 kb on - strand at 669.800 kb on - strand at 669.800 kb on - strand at 669.801 kb on + strand at 669.801 kb on + strand at 669.802 kb on - strand at 669.821 kb on - strand at 669.831 kb on + strand at 670.067 kb on - strand, within AMB_RS03210 at 670.067 kb on - strand, within AMB_RS03210 at 670.067 kb on - strand, within AMB_RS03210 at 670.067 kb on - strand, within AMB_RS03210 at 670.165 kb on + strand, within AMB_RS03210 at 670.165 kb on + strand, within AMB_RS03210 at 670.165 kb on + strand, within AMB_RS03210 at 670.165 kb on + strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.246 kb on + strand, within AMB_RS03210 at 670.246 kb on + strand, within AMB_RS03210 at 670.247 kb on - strand, within AMB_RS03210 at 670.247 kb on - strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.364 kb on - strand, within AMB_RS03210 at 670.598 kb on + strand, within AMB_RS03210 at 670.787 kb on + strand, within AMB_RS03210 at 670.788 kb on - strand, within AMB_RS03210 at 670.867 kb on - strand, within AMB_RS03210 at 670.867 kb on - strand, within AMB_RS03210 at 670.991 kb on - strand at 670.991 kb on - strand at 671.096 kb on + strand at 671.097 kb on - strand at 671.141 kb on + strand at 671.142 kb on - strand at 671.142 kb on - strand at 671.202 kb on - strand, within AMB_RS03215 at 671.287 kb on + strand, within AMB_RS03215 at 671.287 kb on + strand, within AMB_RS03215 at 671.287 kb on + strand, within AMB_RS03215 at 671.601 kb on - strand, within AMB_RS03215 at 671.643 kb on - strand, within AMB_RS03215 at 671.822 kb on + strand, within AMB_RS03215 at 671.823 kb on - strand, within AMB_RS03215 at 671.841 kb on + strand, within AMB_RS03215 at 671.842 kb on - strand, within AMB_RS03215 at 671.843 kb on + strand, within AMB_RS03215 at 671.843 kb on + strand, within AMB_RS03215 at 671.843 kb on + strand, within AMB_RS03215 at 671.843 kb on + strand, within AMB_RS03215 at 671.844 kb on - strand, within AMB_RS03215 at 671.844 kb on - strand, within AMB_RS03215 at 671.924 kb on + strand, within AMB_RS03215 at 671.925 kb on - strand, within AMB_RS03215 at 671.972 kb on + strand, within AMB_RS03215 at 671.973 kb on - strand, within AMB_RS03215 at 671.973 kb on - strand, within AMB_RS03215
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_2 remove 669,119 - AMB_RS03205 0.76 -1.5 669,205 + AMB_RS03205 0.84 -1.0 669,205 + AMB_RS03205 0.84 -2.1 669,206 - AMB_RS03205 0.84 -1.5 669,373 + +0.4 669,374 - -0.7 669,380 + -0.1 669,513 + +0.3 669,513 + -0.8 669,578 + +0.8 669,578 + -3.0 669,578 + -0.2 669,578 + -0.6 669,578 + -0.1 669,578 + -0.8 669,578 + +0.4 669,579 - -1.2 669,579 - -0.9 669,579 - +1.4 669,579 - -1.0 669,579 - -1.3 669,579 - +0.6 669,579 - -0.0 669,579 - +0.6 669,579 - +1.2 669,579 - -1.0 669,791 + +0.1 669,799 + -0.1 669,800 - -0.0 669,800 - -1.0 669,800 - -1.4 669,800 - +1.3 669,801 + +0.1 669,801 + -1.1 669,802 - -0.1 669,821 - -0.2 669,831 + -0.5 670,067 - AMB_RS03210 0.22 -0.3 670,067 - AMB_RS03210 0.22 -0.5 670,067 - AMB_RS03210 0.22 -0.6 670,067 - AMB_RS03210 0.22 -0.1 670,165 + AMB_RS03210 0.30 -0.8 670,165 + AMB_RS03210 0.30 -0.1 670,165 + AMB_RS03210 0.30 +1.0 670,165 + AMB_RS03210 0.30 -0.4 670,166 - AMB_RS03210 0.30 -0.6 670,166 - AMB_RS03210 0.30 -0.6 670,166 - AMB_RS03210 0.30 +0.4 670,166 - AMB_RS03210 0.30 +0.1 670,166 - AMB_RS03210 0.30 +0.6 670,246 + AMB_RS03210 0.37 -1.7 670,246 + AMB_RS03210 0.37 -0.1 670,247 - AMB_RS03210 0.37 -0.1 670,247 - AMB_RS03210 0.37 +0.3 670,363 + AMB_RS03210 0.47 -1.4 670,363 + AMB_RS03210 0.47 +1.7 670,363 + AMB_RS03210 0.47 -0.6 670,363 + AMB_RS03210 0.47 +0.5 670,363 + AMB_RS03210 0.47 +0.7 670,363 + AMB_RS03210 0.47 +0.1 670,364 - AMB_RS03210 0.47 -0.1 670,598 + AMB_RS03210 0.67 +1.6 670,787 + AMB_RS03210 0.83 +0.1 670,788 - AMB_RS03210 0.83 +0.8 670,867 - AMB_RS03210 0.89 -0.2 670,867 - AMB_RS03210 0.89 -1.8 670,991 - -0.5 670,991 - +0.2 671,096 + -0.2 671,097 - -0.5 671,141 + -2.5 671,142 - +0.7 671,142 - -1.9 671,202 - AMB_RS03215 0.10 +0.7 671,287 + AMB_RS03215 0.18 -0.7 671,287 + AMB_RS03215 0.18 -1.4 671,287 + AMB_RS03215 0.18 +1.9 671,601 - AMB_RS03215 0.48 -0.5 671,643 - AMB_RS03215 0.52 -0.4 671,822 + AMB_RS03215 0.69 +0.5 671,823 - AMB_RS03215 0.70 -1.1 671,841 + AMB_RS03215 0.71 -0.0 671,842 - AMB_RS03215 0.71 -0.0 671,843 + AMB_RS03215 0.71 +0.3 671,843 + AMB_RS03215 0.71 +0.1 671,843 + AMB_RS03215 0.71 -0.6 671,843 + AMB_RS03215 0.71 +0.8 671,844 - AMB_RS03215 0.72 -0.0 671,844 - AMB_RS03215 0.72 -0.8 671,924 + AMB_RS03215 0.79 -1.5 671,925 - AMB_RS03215 0.79 -0.2 671,972 + AMB_RS03215 0.84 +0.1 671,973 - AMB_RS03215 0.84 -2.5 671,973 - AMB_RS03215 0.84 -1.5
Or see this region's nucleotide sequence