Experiment: 30_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02075 and AMB_RS02080 overlap by 4 nucleotides AMB_RS02080 and AMB_RS02085 are separated by 262 nucleotides AMB_RS02085 and AMB_RS02090 are separated by 43 nucleotides AMB_RS02090 and AMB_RS02095 are separated by 15 nucleotides
AMB_RS02075: AMB_RS02075 - IS66 family insertion sequence hypothetical protein, at 437,227 to 437,574
_RS02075
AMB_RS02080: AMB_RS02080 - IS66 family insertion sequence hypothetical protein, at 437,571 to 437,945
_RS02080
AMB_RS02085: AMB_RS02085 - hypothetical protein, at 438,208 to 438,459
_RS02085
AMB_RS02090: AMB_RS02090 - DDE transposase, at 438,503 to 438,945
_RS02090
AMB_RS02095: AMB_RS02095 - IS630 family transposase, at 438,961 to 439,396
_RS02095
Position (kb) 438
439 Strain fitness (log2 ratio) -2
-1
0
1 at 438.005 kb on + strand at 438.005 kb on + strand at 438.136 kb on + strand at 438.137 kb on - strand at 438.137 kb on - strand at 438.313 kb on - strand, within AMB_RS02085 at 438.313 kb on - strand, within AMB_RS02085 at 438.326 kb on - strand, within AMB_RS02085 at 438.326 kb on - strand, within AMB_RS02085 at 438.370 kb on + strand, within AMB_RS02085 at 438.371 kb on - strand, within AMB_RS02085 at 438.433 kb on + strand, within AMB_RS02085 at 438.618 kb on + strand, within AMB_RS02090 at 438.619 kb on - strand, within AMB_RS02090 at 438.687 kb on + strand, within AMB_RS02090 at 438.721 kb on + strand, within AMB_RS02090 at 438.722 kb on - strand, within AMB_RS02090 at 438.770 kb on + strand, within AMB_RS02090 at 438.770 kb on + strand, within AMB_RS02090 at 438.770 kb on + strand, within AMB_RS02090 at 438.770 kb on + strand, within AMB_RS02090 at 438.770 kb on + strand, within AMB_RS02090 at 438.770 kb on + strand, within AMB_RS02090 at 438.771 kb on - strand, within AMB_RS02090 at 438.771 kb on - strand, within AMB_RS02090 at 438.771 kb on - strand, within AMB_RS02090 at 438.771 kb on - strand, within AMB_RS02090 at 438.771 kb on - strand, within AMB_RS02090 at 438.771 kb on - strand, within AMB_RS02090 at 438.796 kb on - strand, within AMB_RS02090 at 438.943 kb on + strand at 438.943 kb on + strand at 438.943 kb on + strand at 438.944 kb on - strand at 438.944 kb on - strand at 438.945 kb on + strand at 438.945 kb on + strand at 438.945 kb on + strand at 438.945 kb on + strand at 438.945 kb on + strand at 438.945 kb on + strand at 438.945 kb on + strand at 438.946 kb on - strand at 438.946 kb on - strand at 438.969 kb on + strand at 438.969 kb on + strand at 439.056 kb on + strand, within AMB_RS02095 at 439.056 kb on + strand, within AMB_RS02095 at 439.057 kb on - strand, within AMB_RS02095 at 439.057 kb on - strand, within AMB_RS02095 at 439.057 kb on - strand, within AMB_RS02095 at 439.065 kb on - strand, within AMB_RS02095 at 439.065 kb on - strand, within AMB_RS02095 at 439.065 kb on - strand, within AMB_RS02095 at 439.096 kb on - strand, within AMB_RS02095 at 439.096 kb on - strand, within AMB_RS02095 at 439.289 kb on - strand, within AMB_RS02095 at 439.289 kb on - strand, within AMB_RS02095 at 439.362 kb on + strand at 439.362 kb on + strand at 439.363 kb on - strand at 439.411 kb on - strand at 439.411 kb on - strand at 439.411 kb on - strand at 439.411 kb on - strand at 439.411 kb on - strand at 439.411 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_2 remove 438,005 + -0.3 438,005 + -0.3 438,136 + +0.6 438,137 - -1.5 438,137 - -0.7 438,313 - AMB_RS02085 0.42 -0.0 438,313 - AMB_RS02085 0.42 -0.2 438,326 - AMB_RS02085 0.47 -0.0 438,326 - AMB_RS02085 0.47 -0.0 438,370 + AMB_RS02085 0.64 -1.1 438,371 - AMB_RS02085 0.65 -0.7 438,433 + AMB_RS02085 0.89 -1.0 438,618 + AMB_RS02090 0.26 +0.1 438,619 - AMB_RS02090 0.26 -0.0 438,687 + AMB_RS02090 0.42 +0.0 438,721 + AMB_RS02090 0.49 -0.8 438,722 - AMB_RS02090 0.49 -1.3 438,770 + AMB_RS02090 0.60 -0.8 438,770 + AMB_RS02090 0.60 -0.6 438,770 + AMB_RS02090 0.60 -0.3 438,770 + AMB_RS02090 0.60 +0.1 438,770 + AMB_RS02090 0.60 -0.3 438,770 + AMB_RS02090 0.60 -0.1 438,771 - AMB_RS02090 0.60 -0.6 438,771 - AMB_RS02090 0.60 -0.3 438,771 - AMB_RS02090 0.60 -0.2 438,771 - AMB_RS02090 0.60 -0.5 438,771 - AMB_RS02090 0.60 -0.6 438,771 - AMB_RS02090 0.60 -0.3 438,796 - AMB_RS02090 0.66 +0.0 438,943 + -0.6 438,943 + +0.6 438,943 + -1.5 438,944 - -0.7 438,944 - -0.1 438,945 + -0.2 438,945 + -1.9 438,945 + +0.1 438,945 + +0.8 438,945 + -1.1 438,945 + -0.5 438,945 + +0.1 438,946 - -0.6 438,946 - -0.1 438,969 + -0.5 438,969 + -1.3 439,056 + AMB_RS02095 0.22 -0.2 439,056 + AMB_RS02095 0.22 -0.8 439,057 - AMB_RS02095 0.22 -0.3 439,057 - AMB_RS02095 0.22 -0.1 439,057 - AMB_RS02095 0.22 -1.3 439,065 - AMB_RS02095 0.24 -1.9 439,065 - AMB_RS02095 0.24 -0.3 439,065 - AMB_RS02095 0.24 -1.7 439,096 - AMB_RS02095 0.31 -0.4 439,096 - AMB_RS02095 0.31 +1.0 439,289 - AMB_RS02095 0.75 +0.1 439,289 - AMB_RS02095 0.75 -0.3 439,362 + -1.0 439,362 + -1.0 439,363 - +1.7 439,411 - -0.5 439,411 - -0.7 439,411 - -0.6 439,411 - -0.1 439,411 - +0.1 439,411 - -0.0
Or see this region's nucleotide sequence