Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS15305 and ABID97_RS15310 are separated by 10 nucleotides ABID97_RS15310 and ABID97_RS15315 overlap by 4 nucleotides ABID97_RS15315 and ABID97_RS15320 are separated by 54 nucleotides ABID97_RS15320 and ABID97_RS15325 overlap by 4 nucleotides
ABID97_RS15305: ABID97_RS15305 - TonB-dependent siderophore receptor, at 3,165,994 to 3,168,150
_RS15305
ABID97_RS15310: ABID97_RS15310 - thiamine phosphate synthase, at 3,168,161 to 3,169,093
_RS15310
ABID97_RS15315: ABID97_RS15315 - thiazole synthase, at 3,169,090 to 3,169,893
_RS15315
ABID97_RS15320: ABID97_RS15320 - sulfur carrier protein ThiS, at 3,169,948 to 3,170,151
_RS15320
ABID97_RS15325: ABID97_RS15325 - FAD-dependent oxidoreductase, at 3,170,148 to 3,171,239
_RS15325
Position (kb)
3169
3170 Strain fitness (log2 ratio)
-1
0
1
2 at 3168.424 kb on - strand, within ABID97_RS15310 at 3168.424 kb on - strand, within ABID97_RS15310 at 3168.969 kb on + strand, within ABID97_RS15310 at 3169.065 kb on - strand at 3169.275 kb on - strand, within ABID97_RS15315 at 3169.481 kb on + strand, within ABID97_RS15315 at 3169.481 kb on + strand, within ABID97_RS15315 at 3169.559 kb on + strand, within ABID97_RS15315 at 3169.820 kb on + strand at 3169.933 kb on + strand at 3170.141 kb on + strand at 3170.500 kb on + strand, within ABID97_RS15325 at 3170.501 kb on - strand, within ABID97_RS15325
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 3,168,424 - ABID97_RS15310 0.28 -0.6 3,168,424 - ABID97_RS15310 0.28 -0.7 3,168,969 + ABID97_RS15310 0.87 -0.5 3,169,065 - +0.1 3,169,275 - ABID97_RS15315 0.23 +0.4 3,169,481 + ABID97_RS15315 0.49 +0.0 3,169,481 + ABID97_RS15315 0.49 -0.7 3,169,559 + ABID97_RS15315 0.58 +0.2 3,169,820 + -0.4 3,169,933 + -0.3 3,170,141 + +2.7 3,170,500 + ABID97_RS15325 0.32 +0.0 3,170,501 - ABID97_RS15325 0.32 -0.8
Or see this region's nucleotide sequence