Experiment: Glycolic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS03730 and ABID97_RS03735 are separated by 13 nucleotides ABID97_RS03735 and ABID97_RS03740 overlap by 4 nucleotides ABID97_RS03740 and ABID97_RS03745 are separated by 105 nucleotides
ABID97_RS03730: ABID97_RS03730 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 767,089 to 767,493
_RS03730
ABID97_RS03735: ABID97_RS03735 - extradiol ring-cleavage dioxygenase, at 767,507 to 768,334
_RS03735
ABID97_RS03740: ABID97_RS03740 - aromatic ring-opening dioxygenase subunit LigA, at 768,331 to 768,666
_RS03740
ABID97_RS03745: ABID97_RS03745 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 768,772 to 770,235
_RS03745
Position (kb)
768
769 Strain fitness (log2 ratio)
-1
0
1
2
3 at 767.471 kb on + strand at 767.472 kb on - strand at 767.472 kb on - strand at 767.671 kb on - strand, within ABID97_RS03735 at 768.108 kb on + strand, within ABID97_RS03735 at 768.108 kb on + strand, within ABID97_RS03735 at 768.334 kb on + strand at 768.334 kb on + strand at 768.398 kb on + strand, within ABID97_RS03740 at 768.500 kb on + strand, within ABID97_RS03740 at 768.500 kb on + strand, within ABID97_RS03740 at 768.501 kb on - strand, within ABID97_RS03740 at 768.657 kb on - strand at 768.793 kb on - strand at 768.793 kb on - strand at 768.845 kb on + strand at 768.846 kb on - strand at 769.151 kb on + strand, within ABID97_RS03745 at 769.152 kb on - strand, within ABID97_RS03745 at 769.152 kb on - strand, within ABID97_RS03745 at 769.244 kb on + strand, within ABID97_RS03745 at 769.244 kb on + strand, within ABID97_RS03745 at 769.245 kb on - strand, within ABID97_RS03745 at 769.245 kb on - strand, within ABID97_RS03745 at 769.563 kb on + strand, within ABID97_RS03745
Per-strain Table
Position Strand Gene LocusTag Fraction Glycolic Acid (C) remove 767,471 + -0.8 767,472 - -0.5 767,472 - +0.2 767,671 - ABID97_RS03735 0.20 -0.2 768,108 + ABID97_RS03735 0.73 +0.2 768,108 + ABID97_RS03735 0.73 -0.2 768,334 + +0.3 768,334 + -0.1 768,398 + ABID97_RS03740 0.20 +0.4 768,500 + ABID97_RS03740 0.50 +0.5 768,500 + ABID97_RS03740 0.50 +0.4 768,501 - ABID97_RS03740 0.51 -0.1 768,657 - +3.0 768,793 - +0.6 768,793 - -0.0 768,845 + +0.6 768,846 - +0.1 769,151 + ABID97_RS03745 0.26 +0.5 769,152 - ABID97_RS03745 0.26 +0.1 769,152 - ABID97_RS03745 0.26 +0.4 769,244 + ABID97_RS03745 0.32 -0.0 769,244 + ABID97_RS03745 0.32 +2.0 769,245 - ABID97_RS03745 0.32 +0.2 769,245 - ABID97_RS03745 0.32 +0.2 769,563 + ABID97_RS03745 0.54 +0.4
Or see this region's nucleotide sequence