Experiment: D-Glucose (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapF and yigA overlap by 4 nucleotides yigA and xerC overlap by 4 nucleotides xerC and yigB overlap by 1 nucleotides yigB and uvrD are separated by 83 nucleotides
b3809: dapF - diaminopimelate epimerase (VIMSS), at 3,992,785 to 3,993,609
dapF
b3810: yigA - hypothetical protein (NCBI), at 3,993,606 to 3,994,313
yigA
b3811: xerC - site-specific tyrosine recombinase XerC (NCBI), at 3,994,310 to 3,995,206
xerC
b3812: yigB - predicted hydrolase (NCBI), at 3,995,206 to 3,995,922
yigB
b3813: uvrD - DNA-dependent ATPase I and helicase II (NCBI), at 3,996,006 to 3,998,168
uvrD
Position (kb)
3994
3995
3996 Strain fitness (log2 ratio)
-2
-1
0
1 at 3993.601 kb on + strand at 3993.601 kb on + strand at 3993.637 kb on + strand at 3993.647 kb on - strand at 3993.778 kb on - strand, within yigA at 3993.778 kb on - strand, within yigA at 3993.782 kb on - strand, within yigA at 3993.813 kb on - strand, within yigA at 3993.813 kb on - strand, within yigA at 3993.815 kb on + strand, within yigA at 3993.827 kb on + strand, within yigA at 3993.827 kb on + strand, within yigA at 3993.829 kb on + strand, within yigA at 3993.837 kb on - strand, within yigA at 3993.837 kb on - strand, within yigA at 3993.854 kb on - strand, within yigA at 3993.892 kb on - strand, within yigA at 3993.892 kb on - strand, within yigA at 3993.948 kb on - strand, within yigA at 3993.948 kb on - strand, within yigA at 3993.972 kb on - strand, within yigA at 3993.978 kb on - strand, within yigA at 3994.051 kb on - strand, within yigA at 3994.088 kb on - strand, within yigA at 3994.090 kb on + strand, within yigA at 3994.090 kb on + strand, within yigA at 3994.093 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.170 kb on + strand, within yigA at 3994.192 kb on + strand, within yigA at 3994.192 kb on + strand, within yigA at 3994.202 kb on + strand, within yigA at 3994.210 kb on - strand, within yigA at 3994.279 kb on - strand at 3994.300 kb on - strand at 3994.300 kb on - strand at 3994.314 kb on - strand at 3994.318 kb on + strand at 3994.399 kb on + strand at 3994.765 kb on + strand, within xerC at 3994.776 kb on + strand, within xerC at 3994.932 kb on + strand, within xerC at 3995.087 kb on + strand, within xerC at 3995.093 kb on - strand, within xerC at 3995.093 kb on - strand, within xerC at 3995.095 kb on - strand, within xerC at 3995.113 kb on + strand, within xerC at 3995.165 kb on + strand at 3995.165 kb on + strand at 3995.246 kb on + strand at 3995.246 kb on + strand at 3995.251 kb on + strand at 3995.308 kb on + strand, within yigB at 3995.308 kb on + strand, within yigB at 3995.316 kb on - strand, within yigB at 3995.338 kb on + strand, within yigB at 3995.341 kb on + strand, within yigB at 3995.351 kb on - strand, within yigB at 3995.379 kb on - strand, within yigB at 3995.391 kb on - strand, within yigB at 3995.400 kb on + strand, within yigB at 3995.567 kb on + strand, within yigB at 3995.615 kb on + strand, within yigB at 3995.615 kb on + strand, within yigB at 3995.782 kb on + strand, within yigB at 3995.826 kb on - strand, within yigB at 3995.864 kb on + strand at 3995.875 kb on - strand at 3995.875 kb on - strand at 3995.950 kb on - strand at 3995.962 kb on + strand at 3996.013 kb on + strand at 3996.013 kb on + strand at 3996.013 kb on + strand at 3996.013 kb on + strand at 3996.013 kb on + strand at 3996.021 kb on - strand at 3996.037 kb on + strand at 3996.069 kb on + strand at 3996.095 kb on + strand at 3996.103 kb on + strand at 3996.111 kb on - strand at 3996.148 kb on + strand at 3996.150 kb on + strand at 3996.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) MOPS remove 3,993,601 + +0.1 3,993,601 + +0.8 3,993,637 + -1.2 3,993,647 - -0.3 3,993,778 - yigA b3810 0.24 -1.7 3,993,778 - yigA b3810 0.24 +0.6 3,993,782 - yigA b3810 0.25 -1.7 3,993,813 - yigA b3810 0.29 -0.4 3,993,813 - yigA b3810 0.29 -0.5 3,993,815 + yigA b3810 0.30 -0.6 3,993,827 + yigA b3810 0.31 -0.5 3,993,827 + yigA b3810 0.31 +0.5 3,993,829 + yigA b3810 0.31 +0.7 3,993,837 - yigA b3810 0.33 -2.0 3,993,837 - yigA b3810 0.33 -0.9 3,993,854 - yigA b3810 0.35 +1.0 3,993,892 - yigA b3810 0.40 -0.8 3,993,892 - yigA b3810 0.40 +1.1 3,993,948 - yigA b3810 0.48 +0.2 3,993,948 - yigA b3810 0.48 +0.0 3,993,972 - yigA b3810 0.52 -0.2 3,993,978 - yigA b3810 0.53 -1.2 3,994,051 - yigA b3810 0.63 -0.1 3,994,088 - yigA b3810 0.68 +0.1 3,994,090 + yigA b3810 0.68 +0.8 3,994,090 + yigA b3810 0.68 -0.0 3,994,093 - yigA b3810 0.69 -0.2 3,994,098 - yigA b3810 0.69 -0.6 3,994,098 - yigA b3810 0.69 -0.2 3,994,098 - yigA b3810 0.69 -0.1 3,994,098 - yigA b3810 0.69 -0.1 3,994,098 - yigA b3810 0.69 -0.9 3,994,098 - yigA b3810 0.69 -0.1 3,994,170 + yigA b3810 0.80 +0.2 3,994,192 + yigA b3810 0.83 -0.2 3,994,192 + yigA b3810 0.83 +0.4 3,994,202 + yigA b3810 0.84 +0.1 3,994,210 - yigA b3810 0.85 -0.4 3,994,279 - +0.1 3,994,300 - -2.0 3,994,300 - +0.6 3,994,314 - +0.0 3,994,318 + +0.2 3,994,399 + +1.0 3,994,765 + xerC b3811 0.51 -0.5 3,994,776 + xerC b3811 0.52 -0.7 3,994,932 + xerC b3811 0.69 -1.0 3,995,087 + xerC b3811 0.87 -0.9 3,995,093 - xerC b3811 0.87 +0.0 3,995,093 - xerC b3811 0.87 +0.4 3,995,095 - xerC b3811 0.88 +0.0 3,995,113 + xerC b3811 0.90 +0.3 3,995,165 + +1.0 3,995,165 + +0.9 3,995,246 + -0.4 3,995,246 + +0.0 3,995,251 + +0.3 3,995,308 + yigB b3812 0.14 -0.2 3,995,308 + yigB b3812 0.14 +0.3 3,995,316 - yigB b3812 0.15 -0.3 3,995,338 + yigB b3812 0.18 -0.0 3,995,341 + yigB b3812 0.19 -0.5 3,995,351 - yigB b3812 0.20 +0.1 3,995,379 - yigB b3812 0.24 -0.2 3,995,391 - yigB b3812 0.26 -0.6 3,995,400 + yigB b3812 0.27 +0.1 3,995,567 + yigB b3812 0.50 +1.0 3,995,615 + yigB b3812 0.57 -0.1 3,995,615 + yigB b3812 0.57 +1.1 3,995,782 + yigB b3812 0.80 -0.2 3,995,826 - yigB b3812 0.86 +0.8 3,995,864 + -0.2 3,995,875 - +0.1 3,995,875 - -0.3 3,995,950 - -0.6 3,995,962 + +0.5 3,996,013 + +0.8 3,996,013 + +0.2 3,996,013 + -0.5 3,996,013 + -0.3 3,996,013 + -0.3 3,996,021 - -0.1 3,996,037 + -1.0 3,996,069 + +0.4 3,996,095 + +0.5 3,996,103 + -0.7 3,996,111 - -0.4 3,996,148 + +0.5 3,996,150 + +0.1 3,996,180 - -0.3
Or see this region's nucleotide sequence