Experiment: D-Glucose (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsN and yhbJ are separated by 45 nucleotides yhbJ and npr overlap by 4 nucleotides npr and yrbL are separated by 213 nucleotides yrbL and mtgA overlap by 4 nucleotides
b3204: ptsN - sugar-specific enzyme IIA component of PTS (NCBI), at 3,344,600 to 3,345,091
ptsN
b3205: yhbJ - hypothetical protein (NCBI), at 3,345,137 to 3,345,991
yhbJ
b3206: npr - phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI), at 3,345,988 to 3,346,260
npr
b3207: yrbL - hypothetical protein (NCBI), at 3,346,474 to 3,347,106
yrbL
b3208: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI), at 3,347,103 to 3,347,831
mtgA
Position (kb)
3345
3346
3347 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3345.026 kb on + strand, within ptsN at 3345.026 kb on + strand, within ptsN at 3345.026 kb on + strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.066 kb on + strand at 3345.072 kb on + strand at 3345.072 kb on + strand at 3345.102 kb on - strand at 3345.102 kb on - strand at 3345.102 kb on - strand at 3345.116 kb on - strand at 3345.116 kb on - strand at 3345.139 kb on - strand at 3345.140 kb on + strand at 3345.231 kb on + strand, within yhbJ at 3345.249 kb on - strand, within yhbJ at 3345.264 kb on - strand, within yhbJ at 3345.264 kb on - strand, within yhbJ at 3345.305 kb on - strand, within yhbJ at 3345.343 kb on + strand, within yhbJ at 3345.396 kb on - strand, within yhbJ at 3345.698 kb on + strand, within yhbJ at 3345.698 kb on + strand, within yhbJ at 3345.735 kb on + strand, within yhbJ at 3345.863 kb on - strand, within yhbJ at 3345.863 kb on - strand, within yhbJ at 3345.892 kb on - strand, within yhbJ at 3345.892 kb on - strand, within yhbJ at 3345.909 kb on - strand at 3345.953 kb on + strand at 3346.034 kb on - strand, within npr at 3346.147 kb on + strand, within npr at 3346.173 kb on - strand, within npr at 3346.174 kb on + strand, within npr at 3346.174 kb on + strand, within npr at 3346.186 kb on - strand, within npr at 3346.196 kb on - strand, within npr at 3346.286 kb on + strand at 3346.286 kb on + strand at 3346.444 kb on - strand at 3346.487 kb on - strand at 3346.655 kb on + strand, within yrbL at 3346.688 kb on - strand, within yrbL at 3346.697 kb on - strand, within yrbL at 3346.795 kb on + strand, within yrbL at 3346.809 kb on + strand, within yrbL at 3346.921 kb on + strand, within yrbL at 3346.921 kb on + strand, within yrbL at 3346.932 kb on - strand, within yrbL at 3346.965 kb on - strand, within yrbL at 3346.972 kb on + strand, within yrbL at 3346.980 kb on - strand, within yrbL at 3346.980 kb on - strand, within yrbL at 3346.986 kb on + strand, within yrbL at 3346.986 kb on + strand, within yrbL at 3347.046 kb on + strand at 3347.086 kb on - strand at 3347.092 kb on - strand at 3347.111 kb on + strand at 3347.151 kb on - strand at 3347.178 kb on + strand, within mtgA at 3347.178 kb on + strand, within mtgA at 3347.178 kb on + strand, within mtgA at 3347.178 kb on + strand, within mtgA at 3347.184 kb on + strand, within mtgA at 3347.192 kb on - strand, within mtgA at 3347.205 kb on - strand, within mtgA at 3347.205 kb on - strand, within mtgA at 3347.226 kb on + strand, within mtgA at 3347.226 kb on + strand, within mtgA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) MOPS remove 3,345,026 + ptsN b3204 0.87 -0.3 3,345,026 + ptsN b3204 0.87 +1.2 3,345,026 + ptsN b3204 0.87 -1.9 3,345,034 - ptsN b3204 0.88 -0.4 3,345,034 - ptsN b3204 0.88 -2.2 3,345,034 - ptsN b3204 0.88 -0.7 3,345,034 - ptsN b3204 0.88 -0.9 3,345,066 + +0.3 3,345,072 + +0.4 3,345,072 + -0.1 3,345,102 - -1.3 3,345,102 - -0.8 3,345,102 - -0.6 3,345,116 - +1.6 3,345,116 - -0.0 3,345,139 - +0.8 3,345,140 + +0.5 3,345,231 + yhbJ b3205 0.11 +1.4 3,345,249 - yhbJ b3205 0.13 -0.5 3,345,264 - yhbJ b3205 0.15 +0.3 3,345,264 - yhbJ b3205 0.15 +1.9 3,345,305 - yhbJ b3205 0.20 -0.5 3,345,343 + yhbJ b3205 0.24 -0.6 3,345,396 - yhbJ b3205 0.30 -0.5 3,345,698 + yhbJ b3205 0.66 +0.4 3,345,698 + yhbJ b3205 0.66 +1.6 3,345,735 + yhbJ b3205 0.70 -0.1 3,345,863 - yhbJ b3205 0.85 -0.8 3,345,863 - yhbJ b3205 0.85 -0.3 3,345,892 - yhbJ b3205 0.88 -0.4 3,345,892 - yhbJ b3205 0.88 +1.2 3,345,909 - +0.2 3,345,953 + -0.5 3,346,034 - npr b3206 0.17 +0.1 3,346,147 + npr b3206 0.58 -0.9 3,346,173 - npr b3206 0.68 -0.8 3,346,174 + npr b3206 0.68 -0.6 3,346,174 + npr b3206 0.68 -1.1 3,346,186 - npr b3206 0.73 -0.3 3,346,196 - npr b3206 0.76 -0.7 3,346,286 + +0.6 3,346,286 + -0.8 3,346,444 - +0.4 3,346,487 - +0.2 3,346,655 + yrbL b3207 0.29 -0.2 3,346,688 - yrbL b3207 0.34 +0.4 3,346,697 - yrbL b3207 0.35 -0.0 3,346,795 + yrbL b3207 0.51 +0.3 3,346,809 + yrbL b3207 0.53 +0.2 3,346,921 + yrbL b3207 0.71 +0.5 3,346,921 + yrbL b3207 0.71 -0.0 3,346,932 - yrbL b3207 0.72 -0.8 3,346,965 - yrbL b3207 0.78 -0.9 3,346,972 + yrbL b3207 0.79 +0.0 3,346,980 - yrbL b3207 0.80 +0.2 3,346,980 - yrbL b3207 0.80 +0.6 3,346,986 + yrbL b3207 0.81 +0.2 3,346,986 + yrbL b3207 0.81 +0.3 3,347,046 + -0.2 3,347,086 - -0.3 3,347,092 - -0.6 3,347,111 + -0.1 3,347,151 - +0.4 3,347,178 + mtgA b3208 0.10 -0.4 3,347,178 + mtgA b3208 0.10 +0.1 3,347,178 + mtgA b3208 0.10 +0.0 3,347,178 + mtgA b3208 0.10 -0.5 3,347,184 + mtgA b3208 0.11 -0.0 3,347,192 - mtgA b3208 0.12 -0.4 3,347,205 - mtgA b3208 0.14 -0.2 3,347,205 - mtgA b3208 0.14 +0.2 3,347,226 + mtgA b3208 0.17 +0.5 3,347,226 + mtgA b3208 0.17 +0.2
Or see this region's nucleotide sequence