Experiment: D-Glucose (C) MOPS 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt rpoN and yhbH are separated by 22 nucleotides yhbH and ptsN are separated by 117 nucleotides ptsN and yhbJ are separated by 45 nucleotides yhbJ and npr overlap by 4 nucleotides 
        b3202: rpoN - DNA-directed RNA polymerase subunit N (NCBI), at 3,342,739 to 3,344,172 
        rpoN 
         
        
        b3203: yhbH - predicted ribosome-associated, sigma 54 modulation protein (NCBI), at 3,344,195 to 3,344,482 
        yhbH 
         
        
        b3204: ptsN - sugar-specific enzyme IIA component of PTS (NCBI), at 3,344,600 to 3,345,091 
        ptsN 
         
        
        b3205: yhbJ - hypothetical protein (NCBI), at 3,345,137 to 3,345,991 
        yhbJ 
         
        
        b3206: npr - phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI), at 3,345,988 to 3,346,260 
        npr 
         Position (kb) 3344 
3345 
3346 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 3344.190 kb on + strand at 3344.190 kb on + strand at 3344.193 kb on - strand at 3344.193 kb on - strand at 3344.195 kb on + strand at 3344.201 kb on - strand at 3344.302 kb on + strand, within yhbH at 3344.302 kb on + strand, within yhbH at 3344.310 kb on - strand, within yhbH at 3344.345 kb on + strand, within yhbH at 3344.346 kb on - strand, within yhbH at 3344.354 kb on - strand, within yhbH at 3344.354 kb on - strand, within yhbH at 3344.476 kb on + strand at 3344.502 kb on + strand at 3344.514 kb on + strand at 3344.522 kb on - strand at 3344.524 kb on - strand at 3344.524 kb on - strand at 3344.545 kb on + strand at 3344.554 kb on - strand at 3344.626 kb on + strand at 3344.636 kb on + strand at 3344.636 kb on + strand at 3344.784 kb on + strand, within ptsN at 3344.801 kb on + strand, within ptsN at 3344.810 kb on - strand, within ptsN at 3344.813 kb on + strand, within ptsN at 3344.813 kb on - strand, within ptsN at 3344.883 kb on - strand, within ptsN at 3344.914 kb on - strand, within ptsN at 3344.929 kb on + strand, within ptsN at 3344.933 kb on + strand, within ptsN at 3344.967 kb on - strand, within ptsN at 3344.967 kb on - strand, within ptsN at 3345.026 kb on + strand, within ptsN at 3345.026 kb on + strand, within ptsN at 3345.026 kb on + strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.066 kb on + strand at 3345.072 kb on + strand at 3345.072 kb on + strand at 3345.102 kb on - strand at 3345.102 kb on - strand at 3345.102 kb on - strand at 3345.116 kb on - strand at 3345.116 kb on - strand at 3345.139 kb on - strand at 3345.140 kb on + strand at 3345.231 kb on + strand, within yhbJ at 3345.249 kb on - strand, within yhbJ at 3345.264 kb on - strand, within yhbJ at 3345.264 kb on - strand, within yhbJ at 3345.305 kb on - strand, within yhbJ at 3345.343 kb on + strand, within yhbJ at 3345.396 kb on - strand, within yhbJ at 3345.698 kb on + strand, within yhbJ at 3345.698 kb on + strand, within yhbJ at 3345.735 kb on + strand, within yhbJ at 3345.863 kb on - strand, within yhbJ at 3345.863 kb on - strand, within yhbJ at 3345.892 kb on - strand, within yhbJ at 3345.892 kb on - strand, within yhbJ at 3345.909 kb on - strand at 3345.953 kb on + strand at 3346.034 kb on - strand, within npr  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction D-Glucose (C) MOPS remove 3,344,190 +  +0.1 3,344,190 +  +0.6 3,344,193 -  -0.4 3,344,193 -  +0.6 3,344,195 +  +0.6 3,344,201 -  -0.6 3,344,302 +  yhbH  b3203 0.37  -0.2 3,344,302 +  yhbH  b3203 0.37  -0.1 3,344,310 -  yhbH  b3203 0.40  -0.1 3,344,345 +  yhbH  b3203 0.52  -0.5 3,344,346 -  yhbH  b3203 0.52  +0.8 3,344,354 -  yhbH  b3203 0.55  +0.3 3,344,354 -  yhbH  b3203 0.55  -0.7 3,344,476 +  -0.3 3,344,502 +  -0.1 3,344,514 +  +0.5 3,344,522 -  -0.2 3,344,524 -  +0.6 3,344,524 -  -0.2 3,344,545 +  +0.5 3,344,554 -  -0.3 3,344,626 +  -0.2 3,344,636 +  -1.0 3,344,636 +  -0.6 3,344,784 +  ptsN  b3204 0.37  -0.7 3,344,801 +  ptsN  b3204 0.41  +0.0 3,344,810 -  ptsN  b3204 0.43  -0.9 3,344,813 +  ptsN  b3204 0.43  -1.8 3,344,813 -  ptsN  b3204 0.43  +0.4 3,344,883 -  ptsN  b3204 0.58  +0.1 3,344,914 -  ptsN  b3204 0.64  +1.0 3,344,929 +  ptsN  b3204 0.67  -1.9 3,344,933 +  ptsN  b3204 0.68  -2.1 3,344,967 -  ptsN  b3204 0.75  +0.3 3,344,967 -  ptsN  b3204 0.75  -0.9 3,345,026 +  ptsN  b3204 0.87  -0.3 3,345,026 +  ptsN  b3204 0.87  +1.2 3,345,026 +  ptsN  b3204 0.87  -1.9 3,345,034 -  ptsN  b3204 0.88  -0.4 3,345,034 -  ptsN  b3204 0.88  -2.2 3,345,034 -  ptsN  b3204 0.88  -0.7 3,345,034 -  ptsN  b3204 0.88  -0.9 3,345,066 +  +0.3 3,345,072 +  +0.4 3,345,072 +  -0.1 3,345,102 -  -1.3 3,345,102 -  -0.8 3,345,102 -  -0.6 3,345,116 -  +1.6 3,345,116 -  -0.0 3,345,139 -  +0.8 3,345,140 +  +0.5 3,345,231 +  yhbJ  b3205 0.11  +1.4 3,345,249 -  yhbJ  b3205 0.13  -0.5 3,345,264 -  yhbJ  b3205 0.15  +0.3 3,345,264 -  yhbJ  b3205 0.15  +1.9 3,345,305 -  yhbJ  b3205 0.20  -0.5 3,345,343 +  yhbJ  b3205 0.24  -0.6 3,345,396 -  yhbJ  b3205 0.30  -0.5 3,345,698 +  yhbJ  b3205 0.66  +0.4 3,345,698 +  yhbJ  b3205 0.66  +1.6 3,345,735 +  yhbJ  b3205 0.70  -0.1 3,345,863 -  yhbJ  b3205 0.85  -0.8 3,345,863 -  yhbJ  b3205 0.85  -0.3 3,345,892 -  yhbJ  b3205 0.88  -0.4 3,345,892 -  yhbJ  b3205 0.88  +1.2 3,345,909 -  +0.2 3,345,953 +  -0.5 3,346,034 -  npr  b3206 0.17  +0.1 
Or see this region's nucleotide sequence