Experiment: D-Glucose (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliD and fliS are separated by 24 nucleotides fliS and fliT overlap by 1 nucleotides fliT and amyA are separated by 77 nucleotides
b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302
fliD
b1925: fliS - flagellar protein FliS (NCBI), at 2,003,327 to 2,003,737
fliS
b1926: fliT - predicted chaperone (NCBI), at 2,003,737 to 2,004,102
fliT
b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667
amyA
Position (kb)
2003
2004
2005 Strain fitness (log2 ratio)
-2
-1
0
1 at 2002.829 kb on - strand, within fliD at 2002.892 kb on - strand, within fliD at 2002.894 kb on - strand, within fliD at 2002.894 kb on - strand, within fliD at 2002.913 kb on - strand, within fliD at 2003.080 kb on - strand, within fliD at 2003.414 kb on + strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.462 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.699 kb on + strand at 2003.734 kb on + strand at 2003.735 kb on - strand at 2003.738 kb on - strand at 2003.923 kb on - strand, within fliT at 2003.933 kb on - strand, within fliT at 2004.035 kb on + strand, within fliT at 2004.073 kb on + strand at 2004.135 kb on + strand at 2004.148 kb on + strand at 2004.241 kb on + strand at 2004.241 kb on + strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.299 kb on + strand at 2004.446 kb on + strand, within amyA at 2004.461 kb on - strand, within amyA at 2004.533 kb on + strand, within amyA at 2004.543 kb on - strand, within amyA at 2004.614 kb on - strand, within amyA at 2004.614 kb on - strand, within amyA at 2004.739 kb on - strand, within amyA at 2004.809 kb on + strand, within amyA at 2004.861 kb on - strand, within amyA at 2004.942 kb on - strand, within amyA at 2005.017 kb on - strand, within amyA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) MOPS remove 2,002,829 - fliD b1924 0.66 -0.9 2,002,892 - fliD b1924 0.71 +0.9 2,002,894 - fliD b1924 0.71 +1.0 2,002,894 - fliD b1924 0.71 +0.0 2,002,913 - fliD b1924 0.72 -1.7 2,003,080 - fliD b1924 0.84 +0.5 2,003,414 + fliS b1925 0.21 +0.2 2,003,451 - fliS b1925 0.30 -0.4 2,003,451 - fliS b1925 0.30 +1.4 2,003,451 - fliS b1925 0.30 +0.2 2,003,462 + fliS b1925 0.33 +0.1 2,003,544 + fliS b1925 0.53 -1.0 2,003,544 + fliS b1925 0.53 +0.6 2,003,699 + +0.4 2,003,734 + -0.3 2,003,735 - +0.0 2,003,738 - +0.4 2,003,923 - fliT b1926 0.51 -0.7 2,003,933 - fliT b1926 0.54 -1.9 2,004,035 + fliT b1926 0.81 -0.2 2,004,073 + +0.4 2,004,135 + +0.7 2,004,148 + -0.3 2,004,241 + -0.3 2,004,241 + +0.3 2,004,249 - -0.2 2,004,249 - -0.8 2,004,249 - -1.2 2,004,299 + -0.2 2,004,446 + amyA b1927 0.18 +0.4 2,004,461 - amyA b1927 0.19 -0.2 2,004,533 + amyA b1927 0.24 -0.0 2,004,543 - amyA b1927 0.24 +0.2 2,004,614 - amyA b1927 0.29 +0.4 2,004,614 - amyA b1927 0.29 -0.3 2,004,739 - amyA b1927 0.38 +0.2 2,004,809 + amyA b1927 0.42 +0.9 2,004,861 - amyA b1927 0.46 +0.9 2,004,942 - amyA b1927 0.51 -0.4 2,005,017 - amyA b1927 0.56 +1.0
Or see this region's nucleotide sequence