Experiment: D-Glucose (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt stfE and pin are separated by 65 nucleotides pin and mcrA are separated by 106 nucleotides mcrA and icdC are separated by 233 nucleotides icdC and elbA are separated by 102 nucleotides
b1157: stfE - no description, at 1,208,342 to 1,208,842
stfE
b1158: pin - e14 prophage; site-specific DNA recombinase (NCBI), at 1,208,908 to 1,209,462
pin
b1159: mcrA - e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI), at 1,209,569 to 1,210,402
mcrA
b4519: icdC - no description, at 1,210,636 to 1,210,800
icdC
b1160: elbA - hypothetical protein (NCBI), at 1,210,903 to 1,211,226
elbA
Position (kb)
1209
1210
1211 Strain fitness (log2 ratio)
-1
0
1
2 at 1208.575 kb on - strand, within stfE at 1208.576 kb on - strand, within stfE at 1208.586 kb on - strand, within stfE at 1208.604 kb on - strand, within stfE at 1208.604 kb on - strand, within stfE at 1208.604 kb on - strand, within stfE at 1208.606 kb on - strand, within stfE at 1208.609 kb on - strand, within stfE at 1208.638 kb on - strand, within stfE at 1208.642 kb on - strand, within stfE at 1208.642 kb on - strand, within stfE at 1208.642 kb on - strand, within stfE at 1208.648 kb on + strand, within stfE at 1208.752 kb on + strand, within stfE at 1208.756 kb on - strand, within stfE at 1208.791 kb on - strand, within stfE at 1208.819 kb on + strand at 1208.838 kb on - strand at 1208.899 kb on - strand at 1208.946 kb on + strand at 1208.946 kb on + strand at 1208.964 kb on - strand, within pin at 1208.964 kb on - strand, within pin at 1208.968 kb on + strand, within pin at 1209.015 kb on - strand, within pin at 1209.110 kb on - strand, within pin at 1209.296 kb on + strand, within pin at 1209.296 kb on + strand, within pin at 1209.306 kb on + strand, within pin at 1209.306 kb on + strand at 1209.316 kb on + strand, within pin at 1209.316 kb on + strand, within pin at 1209.333 kb on + strand, within pin at 1209.348 kb on + strand, within pin at 1209.397 kb on + strand, within pin at 1209.408 kb on + strand at 1209.423 kb on + strand at 1209.612 kb on + strand at 1209.612 kb on + strand at 1209.620 kb on + strand at 1209.635 kb on + strand at 1209.648 kb on + strand at 1209.648 kb on + strand at 1209.663 kb on + strand, within mcrA at 1209.743 kb on + strand, within mcrA at 1209.769 kb on + strand, within mcrA at 1210.309 kb on + strand, within mcrA at 1210.442 kb on - strand at 1210.631 kb on + strand at 1210.631 kb on + strand at 1210.646 kb on + strand at 1210.646 kb on + strand at 1210.646 kb on + strand at 1210.655 kb on + strand, within icdC at 1210.664 kb on + strand, within icdC at 1210.666 kb on + strand, within icdC at 1210.666 kb on + strand, within icdC at 1210.684 kb on + strand, within icdC at 1210.710 kb on + strand, within icdC at 1210.733 kb on + strand, within icdC at 1210.745 kb on + strand, within icdC at 1210.753 kb on + strand, within icdC at 1210.753 kb on + strand, within icdC at 1210.753 kb on + strand, within icdC at 1210.753 kb on + strand, within icdC at 1210.769 kb on + strand, within icdC at 1210.794 kb on + strand at 1210.812 kb on + strand at 1210.812 kb on + strand at 1210.982 kb on - strand, within elbA at 1210.987 kb on - strand, within elbA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) MOPS remove 1,208,575 - stfE b1157 0.47 -0.3 1,208,576 - stfE b1157 0.47 -0.4 1,208,586 - stfE b1157 0.49 -0.1 1,208,604 - stfE b1157 0.52 +0.6 1,208,604 - stfE b1157 0.52 -0.2 1,208,604 - stfE b1157 0.52 +0.3 1,208,606 - stfE b1157 0.53 +0.2 1,208,609 - stfE b1157 0.53 +0.0 1,208,638 - stfE b1157 0.59 -0.8 1,208,642 - stfE b1157 0.60 -0.3 1,208,642 - stfE b1157 0.60 -0.4 1,208,642 - stfE b1157 0.60 +0.6 1,208,648 + stfE b1157 0.61 -0.4 1,208,752 + stfE b1157 0.82 +0.2 1,208,756 - stfE b1157 0.83 -0.1 1,208,791 - stfE b1157 0.90 +0.5 1,208,819 + +0.5 1,208,838 - +0.3 1,208,899 - -0.3 1,208,946 + +0.8 1,208,946 + -0.8 1,208,964 - pin b1158 0.10 -1.1 1,208,964 - pin b1158 0.10 +2.2 1,208,968 + pin b1158 0.11 +0.2 1,209,015 - pin b1158 0.19 +0.4 1,209,110 - pin b1158 0.36 -0.2 1,209,296 + pin b1158 0.70 +1.6 1,209,296 + pin b1158 0.70 +0.6 1,209,306 + pin b1158 0.72 +1.9 1,209,306 + +0.8 1,209,316 + pin b1158 0.74 +0.3 1,209,316 + pin b1158 0.74 +0.1 1,209,333 + pin b1158 0.77 -0.1 1,209,348 + pin b1158 0.79 +0.4 1,209,397 + pin b1158 0.88 +0.4 1,209,408 + +0.3 1,209,423 + -0.0 1,209,612 + +0.0 1,209,612 + +0.1 1,209,620 + +0.2 1,209,635 + -0.2 1,209,648 + -0.8 1,209,648 + +0.4 1,209,663 + mcrA b1159 0.11 -0.9 1,209,743 + mcrA b1159 0.21 -0.8 1,209,769 + mcrA b1159 0.24 -0.2 1,210,309 + mcrA b1159 0.89 -0.9 1,210,442 - -0.1 1,210,631 + +0.7 1,210,631 + -0.5 1,210,646 + -0.4 1,210,646 + -0.5 1,210,646 + +0.8 1,210,655 + icdC b4519 0.12 +0.6 1,210,664 + icdC b4519 0.17 +0.5 1,210,666 + icdC b4519 0.18 +0.1 1,210,666 + icdC b4519 0.18 +0.3 1,210,684 + icdC b4519 0.29 -0.3 1,210,710 + icdC b4519 0.45 +0.5 1,210,733 + icdC b4519 0.59 +0.4 1,210,745 + icdC b4519 0.66 +1.5 1,210,753 + icdC b4519 0.71 -0.3 1,210,753 + icdC b4519 0.71 +0.6 1,210,753 + icdC b4519 0.71 +0.1 1,210,753 + icdC b4519 0.71 +0.2 1,210,769 + icdC b4519 0.81 -0.9 1,210,794 + +0.4 1,210,812 + -0.6 1,210,812 + -0.1 1,210,982 - elbA b1160 0.24 -0.6 1,210,987 - elbA b1160 0.26 -0.2
Or see this region's nucleotide sequence