Experiment: D-Glucose (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apaG and ksgA are separated by 2 nucleotides ksgA and pdxA overlap by 4 nucleotides pdxA and surA overlap by 1 nucleotides
b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606
apaG
b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430
ksgA
b0052: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI), at 52,427 to 53,416
pdxA
b0053: surA - peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI), at 53,416 to 54,702
surA
Position (kb) 52
53
54 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 51.466 kb on + strand, within apaG at 51.472 kb on + strand, within apaG at 51.512 kb on - strand, within apaG at 51.514 kb on - strand, within apaG at 51.520 kb on - strand, within apaG at 51.524 kb on + strand, within apaG at 51.550 kb on - strand, within apaG at 51.560 kb on + strand, within apaG at 51.567 kb on - strand, within apaG at 51.567 kb on - strand, within apaG at 51.576 kb on + strand at 51.576 kb on + strand at 51.586 kb on - strand at 51.613 kb on + strand at 51.614 kb on - strand at 51.615 kb on + strand at 51.615 kb on + strand at 51.673 kb on - strand at 51.703 kb on + strand, within ksgA at 51.703 kb on + strand, within ksgA at 51.812 kb on - strand, within ksgA at 51.836 kb on + strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.904 kb on + strand, within ksgA at 51.906 kb on + strand, within ksgA at 51.925 kb on - strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.045 kb on + strand, within ksgA at 52.062 kb on + strand, within ksgA at 52.062 kb on + strand at 52.070 kb on - strand, within ksgA at 52.072 kb on + strand, within ksgA at 52.089 kb on - strand, within ksgA at 52.276 kb on + strand, within ksgA at 52.276 kb on + strand, within ksgA at 52.284 kb on + strand, within ksgA at 52.338 kb on + strand, within ksgA at 52.338 kb on + strand, within ksgA at 52.343 kb on - strand, within ksgA at 52.348 kb on - strand at 52.348 kb on - strand at 52.348 kb on - strand at 52.354 kb on + strand at 52.420 kb on - strand at 52.490 kb on + strand at 52.490 kb on + strand at 52.502 kb on + strand at 52.502 kb on + strand at 52.518 kb on - strand at 52.518 kb on - strand at 52.626 kb on + strand, within pdxA at 52.688 kb on - strand, within pdxA at 52.735 kb on + strand, within pdxA at 52.735 kb on + strand, within pdxA at 52.740 kb on - strand, within pdxA at 52.766 kb on - strand, within pdxA at 52.780 kb on - strand, within pdxA at 52.797 kb on - strand, within pdxA at 52.879 kb on - strand, within pdxA at 52.914 kb on + strand, within pdxA at 52.982 kb on - strand, within pdxA at 52.982 kb on - strand, within pdxA at 52.983 kb on + strand, within pdxA at 53.061 kb on - strand, within pdxA at 53.062 kb on - strand, within pdxA at 53.062 kb on - strand at 53.064 kb on + strand, within pdxA at 53.067 kb on - strand, within pdxA at 53.163 kb on - strand, within pdxA at 53.163 kb on - strand, within pdxA at 53.181 kb on - strand, within pdxA at 53.181 kb on - strand, within pdxA at 53.202 kb on - strand, within pdxA at 53.221 kb on + strand, within pdxA at 53.221 kb on + strand, within pdxA at 53.226 kb on - strand, within pdxA at 53.246 kb on - strand, within pdxA at 53.291 kb on + strand, within pdxA at 53.328 kb on + strand at 53.328 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.346 kb on - strand at 53.346 kb on - strand at 53.346 kb on - strand at 53.460 kb on - strand at 53.460 kb on - strand at 53.468 kb on - strand at 53.524 kb on - strand at 53.552 kb on + strand, within surA at 53.554 kb on - strand, within surA at 53.565 kb on - strand, within surA at 53.565 kb on - strand, within surA at 53.591 kb on + strand, within surA at 53.603 kb on + strand, within surA at 53.616 kb on - strand, within surA at 53.633 kb on - strand, within surA at 53.633 kb on - strand, within surA at 53.667 kb on + strand, within surA at 53.695 kb on - strand, within surA at 53.702 kb on - strand, within surA at 53.702 kb on - strand, within surA at 53.704 kb on + strand, within surA at 53.736 kb on + strand, within surA at 53.766 kb on - strand, within surA at 53.766 kb on - strand, within surA at 53.776 kb on - strand, within surA at 53.797 kb on - strand, within surA at 53.802 kb on + strand, within surA at 53.807 kb on - strand, within surA at 53.910 kb on + strand, within surA at 53.991 kb on + strand, within surA at 53.997 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.035 kb on - strand, within surA at 54.048 kb on + strand, within surA at 54.148 kb on + strand, within surA at 54.166 kb on - strand, within surA at 54.166 kb on - strand, within surA at 54.178 kb on + strand, within surA at 54.196 kb on - strand, within surA at 54.205 kb on + strand, within surA at 54.285 kb on - strand, within surA at 54.285 kb on - strand, within surA at 54.406 kb on + strand, within surA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) MOPS remove 51,466 + apaG b0050 0.63 +1.1 51,472 + apaG b0050 0.64 +0.5 51,512 - apaG b0050 0.75 -0.5 51,514 - apaG b0050 0.75 -0.8 51,520 - apaG b0050 0.77 -0.1 51,524 + apaG b0050 0.78 -1.0 51,550 - apaG b0050 0.85 +1.1 51,560 + apaG b0050 0.88 +0.1 51,567 - apaG b0050 0.89 +0.1 51,567 - apaG b0050 0.89 -0.7 51,576 + -1.1 51,576 + +0.1 51,586 - -0.8 51,613 + -0.1 51,614 - -0.7 51,615 + +0.2 51,615 + +0.3 51,673 - -1.7 51,703 + ksgA b0051 0.11 +1.2 51,703 + ksgA b0051 0.11 +0.2 51,812 - ksgA b0051 0.25 -1.6 51,836 + ksgA b0051 0.28 -0.3 51,891 - ksgA b0051 0.34 +0.4 51,891 - ksgA b0051 0.34 -2.0 51,904 + ksgA b0051 0.36 -0.9 51,906 + ksgA b0051 0.36 -1.2 51,925 - ksgA b0051 0.38 +0.1 52,040 + ksgA b0051 0.52 -1.4 52,040 + ksgA b0051 0.52 -0.7 52,040 + ksgA b0051 0.52 -0.8 52,042 + ksgA b0051 0.53 -0.0 52,042 + ksgA b0051 0.53 -0.7 52,042 + ksgA b0051 0.53 -0.2 52,042 - ksgA b0051 0.53 -0.9 52,042 - ksgA b0051 0.53 -0.8 52,045 + ksgA b0051 0.53 +1.0 52,062 + ksgA b0051 0.55 +0.4 52,062 + +0.1 52,070 - ksgA b0051 0.56 -1.0 52,072 + ksgA b0051 0.56 -0.3 52,089 - ksgA b0051 0.58 -0.1 52,276 + ksgA b0051 0.81 -1.1 52,276 + ksgA b0051 0.81 -0.2 52,284 + ksgA b0051 0.82 -0.9 52,338 + ksgA b0051 0.89 +0.3 52,338 + ksgA b0051 0.89 -0.2 52,343 - ksgA b0051 0.89 +0.7 52,348 - -0.2 52,348 - -0.7 52,348 - -1.7 52,354 + -0.5 52,420 - -0.3 52,490 + -1.9 52,490 + -2.4 52,502 + -2.4 52,502 + -3.0 52,518 - -0.8 52,518 - -2.1 52,626 + pdxA b0052 0.20 -2.3 52,688 - pdxA b0052 0.26 -4.2 52,735 + pdxA b0052 0.31 -1.4 52,735 + pdxA b0052 0.31 -1.7 52,740 - pdxA b0052 0.32 -3.1 52,766 - pdxA b0052 0.34 -1.8 52,780 - pdxA b0052 0.36 -3.3 52,797 - pdxA b0052 0.37 -4.1 52,879 - pdxA b0052 0.46 -3.6 52,914 + pdxA b0052 0.49 -1.0 52,982 - pdxA b0052 0.56 -0.3 52,982 - pdxA b0052 0.56 -2.9 52,983 + pdxA b0052 0.56 -4.4 53,061 - pdxA b0052 0.64 -2.3 53,062 - pdxA b0052 0.64 -2.9 53,062 - +0.7 53,064 + pdxA b0052 0.64 -2.9 53,067 - pdxA b0052 0.65 -2.4 53,163 - pdxA b0052 0.74 -2.6 53,163 - pdxA b0052 0.74 -2.5 53,181 - pdxA b0052 0.76 -2.9 53,181 - pdxA b0052 0.76 -3.8 53,202 - pdxA b0052 0.78 -0.9 53,221 + pdxA b0052 0.80 -3.1 53,221 + pdxA b0052 0.80 -2.9 53,226 - pdxA b0052 0.81 -3.4 53,246 - pdxA b0052 0.83 -4.3 53,291 + pdxA b0052 0.87 -3.6 53,328 + -3.0 53,328 + -4.0 53,338 + -2.5 53,338 + -5.0 53,338 + -3.2 53,338 + -2.4 53,346 - -4.8 53,346 - -3.0 53,346 - -5.0 53,460 - +1.3 53,460 - +1.9 53,468 - +1.6 53,524 - +1.7 53,552 + surA b0053 0.11 -0.3 53,554 - surA b0053 0.11 +0.5 53,565 - surA b0053 0.12 +1.7 53,565 - surA b0053 0.12 +0.7 53,591 + surA b0053 0.14 +0.6 53,603 + surA b0053 0.15 +0.7 53,616 - surA b0053 0.16 +0.7 53,633 - surA b0053 0.17 +0.7 53,633 - surA b0053 0.17 -0.7 53,667 + surA b0053 0.20 -2.0 53,695 - surA b0053 0.22 +0.7 53,702 - surA b0053 0.22 -0.0 53,702 - surA b0053 0.22 +2.1 53,704 + surA b0053 0.22 -1.2 53,736 + surA b0053 0.25 -1.2 53,766 - surA b0053 0.27 +0.3 53,766 - surA b0053 0.27 +0.9 53,776 - surA b0053 0.28 -1.3 53,797 - surA b0053 0.30 -0.2 53,802 + surA b0053 0.30 +0.1 53,807 - surA b0053 0.30 -0.7 53,910 + surA b0053 0.38 -1.8 53,991 + surA b0053 0.45 -0.3 53,997 - surA b0053 0.45 +0.7 54,015 - surA b0053 0.47 +2.1 54,015 - surA b0053 0.47 +0.7 54,015 - surA b0053 0.47 +1.1 54,035 - surA b0053 0.48 +1.4 54,048 + surA b0053 0.49 -0.9 54,148 + surA b0053 0.57 -2.4 54,166 - surA b0053 0.58 -0.2 54,166 - surA b0053 0.58 -0.3 54,178 + surA b0053 0.59 -1.2 54,196 - surA b0053 0.61 +1.3 54,205 + surA b0053 0.61 -0.2 54,285 - surA b0053 0.68 +0.7 54,285 - surA b0053 0.68 +1.2 54,406 + surA b0053 0.77 -0.3
Or see this region's nucleotide sequence