Experiment: D-Glucose (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydaN and fnrS are separated by 95 nucleotides fnrS and dbpA are separated by 260 nucleotides
b1342: ydaN - zinc transporter (NCBI), at 1,406,074 to 1,407,057
ydaN
b4699: fnrS - FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq), at 1,407,153 to 1,407,274
fnrS
b1343: dbpA - ATP-dependent RNA helicase, specific for 23S rRNA (NCBI), at 1,407,535 to 1,408,908
dbpA
Position (kb)
1407
1408 Strain fitness (log2 ratio)
-1
0
1
2 at 1406.243 kb on + strand, within ydaN at 1406.396 kb on + strand, within ydaN at 1406.441 kb on + strand, within ydaN at 1406.441 kb on + strand, within ydaN at 1406.471 kb on + strand, within ydaN at 1406.479 kb on - strand, within ydaN at 1406.521 kb on + strand, within ydaN at 1406.521 kb on + strand, within ydaN at 1406.622 kb on + strand, within ydaN at 1406.678 kb on - strand, within ydaN at 1406.727 kb on + strand, within ydaN at 1406.727 kb on + strand, within ydaN at 1406.783 kb on + strand, within ydaN at 1406.812 kb on - strand, within ydaN at 1406.814 kb on - strand, within ydaN at 1406.822 kb on - strand, within ydaN at 1406.876 kb on + strand, within ydaN at 1406.879 kb on - strand, within ydaN at 1406.879 kb on - strand, within ydaN at 1407.150 kb on - strand at 1407.225 kb on - strand, within fnrS at 1407.299 kb on - strand at 1407.384 kb on + strand at 1407.384 kb on + strand at 1407.421 kb on + strand at 1407.429 kb on - strand at 1407.799 kb on + strand, within dbpA at 1408.018 kb on + strand, within dbpA at 1408.018 kb on + strand, within dbpA at 1408.236 kb on + strand, within dbpA at 1408.267 kb on - strand, within dbpA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) MOPS remove 1,406,243 + ydaN b1342 0.17 +0.9 1,406,396 + ydaN b1342 0.33 -0.1 1,406,441 + ydaN b1342 0.37 -0.1 1,406,441 + ydaN b1342 0.37 +0.0 1,406,471 + ydaN b1342 0.40 +0.7 1,406,479 - ydaN b1342 0.41 +0.8 1,406,521 + ydaN b1342 0.45 -0.5 1,406,521 + ydaN b1342 0.45 +0.6 1,406,622 + ydaN b1342 0.56 -0.5 1,406,678 - ydaN b1342 0.61 -0.8 1,406,727 + ydaN b1342 0.66 -0.8 1,406,727 + ydaN b1342 0.66 -0.1 1,406,783 + ydaN b1342 0.72 -1.0 1,406,812 - ydaN b1342 0.75 -0.2 1,406,814 - ydaN b1342 0.75 +1.0 1,406,822 - ydaN b1342 0.76 +0.1 1,406,876 + ydaN b1342 0.82 -0.6 1,406,879 - ydaN b1342 0.82 +1.8 1,406,879 - ydaN b1342 0.82 +0.1 1,407,150 - +0.6 1,407,225 - fnrS b4699 0.59 -0.5 1,407,299 - -0.5 1,407,384 + +0.5 1,407,384 + -0.4 1,407,421 + +0.1 1,407,429 - +0.4 1,407,799 + dbpA b1343 0.19 +0.2 1,408,018 + dbpA b1343 0.35 +0.1 1,408,018 + dbpA b1343 0.35 +0.3 1,408,236 + dbpA b1343 0.51 +1.2 1,408,267 - dbpA b1343 0.53 +0.0
Or see this region's nucleotide sequence