Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4440 and EX28DRAFT_4441 overlap by 4 nucleotides EX28DRAFT_4441 and EX28DRAFT_4442 are separated by 18 nucleotides
EX28DRAFT_4440: EX28DRAFT_4440 - acetolactate synthase, large subunit (EC 2.2.1.6), at 3,911 to 5,557
_4440
EX28DRAFT_4441: EX28DRAFT_4441 - acetolactate synthase, small subunit (EC 2.2.1.6), at 5,554 to 5,817
_4441
EX28DRAFT_4442: EX28DRAFT_4442 - branched-chain amino acid aminotransferase, group I, at 5,836 to 6,765
_4442
Position (kb) 5
6 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4.640 kb on + strand, within EX28DRAFT_4440 at 4.641 kb on - strand, within EX28DRAFT_4440 at 4.641 kb on - strand, within EX28DRAFT_4440 at 4.641 kb on - strand, within EX28DRAFT_4440 at 4.786 kb on + strand, within EX28DRAFT_4440 at 4.787 kb on - strand, within EX28DRAFT_4440 at 4.787 kb on - strand, within EX28DRAFT_4440 at 4.967 kb on + strand, within EX28DRAFT_4440 at 4.968 kb on - strand, within EX28DRAFT_4440 at 5.002 kb on + strand, within EX28DRAFT_4440 at 5.003 kb on - strand, within EX28DRAFT_4440 at 5.003 kb on - strand, within EX28DRAFT_4440 at 5.035 kb on - strand, within EX28DRAFT_4440 at 5.072 kb on + strand, within EX28DRAFT_4440 at 5.073 kb on - strand, within EX28DRAFT_4440 at 5.183 kb on - strand, within EX28DRAFT_4440 at 5.550 kb on + strand at 5.550 kb on + strand at 5.652 kb on + strand, within EX28DRAFT_4441 at 5.653 kb on - strand, within EX28DRAFT_4441 at 5.700 kb on - strand, within EX28DRAFT_4441 at 5.730 kb on - strand, within EX28DRAFT_4441 at 5.736 kb on + strand, within EX28DRAFT_4441 at 5.757 kb on - strand, within EX28DRAFT_4441 at 5.857 kb on + strand at 5.858 kb on - strand at 5.929 kb on + strand, within EX28DRAFT_4442 at 5.929 kb on + strand, within EX28DRAFT_4442 at 5.930 kb on - strand, within EX28DRAFT_4442 at 5.935 kb on - strand, within EX28DRAFT_4442 at 5.942 kb on + strand, within EX28DRAFT_4442 at 5.943 kb on - strand, within EX28DRAFT_4442 at 5.943 kb on - strand, within EX28DRAFT_4442 at 5.962 kb on + strand, within EX28DRAFT_4442 at 5.963 kb on - strand, within EX28DRAFT_4442 at 6.182 kb on - strand, within EX28DRAFT_4442 at 6.184 kb on - strand, within EX28DRAFT_4442 at 6.329 kb on - strand, within EX28DRAFT_4442 at 6.407 kb on - strand, within EX28DRAFT_4442 at 6.407 kb on - strand, within EX28DRAFT_4442 at 6.407 kb on - strand, within EX28DRAFT_4442 at 6.453 kb on + strand, within EX28DRAFT_4442 at 6.546 kb on - strand, within EX28DRAFT_4442 at 6.546 kb on - strand, within EX28DRAFT_4442 at 6.577 kb on + strand, within EX28DRAFT_4442 at 6.688 kb on - strand at 6.732 kb on + strand at 6.733 kb on - strand at 6.737 kb on - strand at 6.756 kb on + strand at 6.764 kb on - strand at 6.769 kb on + strand at 6.770 kb on - strand at 6.797 kb on + strand at 6.798 kb on - strand at 6.801 kb on + strand at 6.802 kb on - strand at 6.802 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 4,640 + EX28DRAFT_4440 0.44 -0.6 4,641 - EX28DRAFT_4440 0.44 -1.3 4,641 - EX28DRAFT_4440 0.44 -2.0 4,641 - EX28DRAFT_4440 0.44 -0.5 4,786 + EX28DRAFT_4440 0.53 -0.9 4,787 - EX28DRAFT_4440 0.53 -1.0 4,787 - EX28DRAFT_4440 0.53 -1.5 4,967 + EX28DRAFT_4440 0.64 +0.2 4,968 - EX28DRAFT_4440 0.64 -0.3 5,002 + EX28DRAFT_4440 0.66 +0.0 5,003 - EX28DRAFT_4440 0.66 -1.2 5,003 - EX28DRAFT_4440 0.66 +0.1 5,035 - EX28DRAFT_4440 0.68 +0.7 5,072 + EX28DRAFT_4440 0.70 +1.2 5,073 - EX28DRAFT_4440 0.71 -2.7 5,183 - EX28DRAFT_4440 0.77 -1.0 5,550 + -1.1 5,550 + +0.1 5,652 + EX28DRAFT_4441 0.37 -1.7 5,653 - EX28DRAFT_4441 0.38 -2.2 5,700 - EX28DRAFT_4441 0.55 -0.2 5,730 - EX28DRAFT_4441 0.67 -1.2 5,736 + EX28DRAFT_4441 0.69 +0.1 5,757 - EX28DRAFT_4441 0.77 -0.4 5,857 + -0.4 5,858 - -3.0 5,929 + EX28DRAFT_4442 0.10 +0.1 5,929 + EX28DRAFT_4442 0.10 +1.1 5,930 - EX28DRAFT_4442 0.10 -1.8 5,935 - EX28DRAFT_4442 0.11 -3.6 5,942 + EX28DRAFT_4442 0.11 -1.0 5,943 - EX28DRAFT_4442 0.12 -3.1 5,943 - EX28DRAFT_4442 0.12 -2.6 5,962 + EX28DRAFT_4442 0.14 -0.7 5,963 - EX28DRAFT_4442 0.14 -3.1 6,182 - EX28DRAFT_4442 0.37 -0.6 6,184 - EX28DRAFT_4442 0.37 -4.0 6,329 - EX28DRAFT_4442 0.53 -3.0 6,407 - EX28DRAFT_4442 0.61 -3.3 6,407 - EX28DRAFT_4442 0.61 -1.9 6,407 - EX28DRAFT_4442 0.61 -2.5 6,453 + EX28DRAFT_4442 0.66 +0.3 6,546 - EX28DRAFT_4442 0.76 -1.0 6,546 - EX28DRAFT_4442 0.76 -2.3 6,577 + EX28DRAFT_4442 0.80 +1.5 6,688 - -0.9 6,732 + -0.4 6,733 - -1.4 6,737 - -0.1 6,756 + -0.4 6,764 - -1.7 6,769 + +0.8 6,770 - -1.5 6,797 + -0.4 6,798 - -2.0 6,801 + +0.0 6,802 - -1.5 6,802 - -2.0
Or see this region's nucleotide sequence