Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4201 and EX28DRAFT_4202 are separated by 50 nucleotides EX28DRAFT_4202 and EX28DRAFT_4203 are separated by 20 nucleotides EX28DRAFT_4203 and EX28DRAFT_4204 are separated by 0 nucleotides EX28DRAFT_4204 and EX28DRAFT_4205 overlap by 4 nucleotides
EX28DRAFT_4201: EX28DRAFT_4201 - phosphonate ABC transporter, periplasmic phosphonate binding protein, at 10,030 to 11,046
_4201
EX28DRAFT_4202: EX28DRAFT_4202 - phosphonate ABC transporter, permease protein PhnE, at 11,097 to 11,876
_4202
EX28DRAFT_4203: EX28DRAFT_4203 - phosphonates metabolism transcriptional regulator PhnF, at 11,897 to 12,622
_4203
EX28DRAFT_4204: EX28DRAFT_4204 - phosphonate C-P lyase system protein PhnG, at 12,623 to 13,075
_4204
EX28DRAFT_4205: EX28DRAFT_4205 - phosphonate C-P lyase system protein PhnH, at 13,072 to 13,656
_4205
Position (kb) 11
12
13 Strain fitness (log2 ratio) -1
0
1 at 11.032 kb on - strand at 11.044 kb on + strand at 11.044 kb on + strand at 11.085 kb on + strand at 11.146 kb on + strand at 11.147 kb on - strand at 11.178 kb on - strand, within EX28DRAFT_4202 at 11.178 kb on - strand, within EX28DRAFT_4202 at 11.290 kb on - strand, within EX28DRAFT_4202 at 11.405 kb on - strand, within EX28DRAFT_4202 at 11.406 kb on + strand, within EX28DRAFT_4202 at 11.623 kb on + strand, within EX28DRAFT_4202 at 11.623 kb on + strand, within EX28DRAFT_4202 at 11.624 kb on - strand, within EX28DRAFT_4202 at 11.658 kb on - strand, within EX28DRAFT_4202 at 12.334 kb on - strand, within EX28DRAFT_4203 at 12.342 kb on - strand, within EX28DRAFT_4203 at 12.569 kb on + strand at 12.869 kb on + strand, within EX28DRAFT_4204 at 12.870 kb on - strand, within EX28DRAFT_4204 at 12.941 kb on + strand, within EX28DRAFT_4204 at 12.942 kb on - strand, within EX28DRAFT_4204 at 13.388 kb on + strand, within EX28DRAFT_4205 at 13.389 kb on - strand, within EX28DRAFT_4205 at 13.389 kb on - strand, within EX28DRAFT_4205 at 13.389 kb on - strand, within EX28DRAFT_4205
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 11,032 - +0.3 11,044 + +0.1 11,044 + -0.1 11,085 + -0.9 11,146 + +0.0 11,147 - +0.2 11,178 - EX28DRAFT_4202 0.10 +0.2 11,178 - EX28DRAFT_4202 0.10 +0.7 11,290 - EX28DRAFT_4202 0.25 +0.6 11,405 - EX28DRAFT_4202 0.39 +1.3 11,406 + EX28DRAFT_4202 0.40 +0.5 11,623 + EX28DRAFT_4202 0.67 +1.1 11,623 + EX28DRAFT_4202 0.67 -0.2 11,624 - EX28DRAFT_4202 0.68 -0.6 11,658 - EX28DRAFT_4202 0.72 -0.3 12,334 - EX28DRAFT_4203 0.60 -1.5 12,342 - EX28DRAFT_4203 0.61 -1.2 12,569 + -0.3 12,869 + EX28DRAFT_4204 0.54 +0.3 12,870 - EX28DRAFT_4204 0.55 +0.4 12,941 + EX28DRAFT_4204 0.70 -0.0 12,942 - EX28DRAFT_4204 0.70 +0.1 13,388 + EX28DRAFT_4205 0.54 -0.0 13,389 - EX28DRAFT_4205 0.54 +0.5 13,389 - EX28DRAFT_4205 0.54 +0.2 13,389 - EX28DRAFT_4205 0.54 +0.1
Or see this region's nucleotide sequence