Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4144 and EX28DRAFT_4145 are separated by 324 nucleotides EX28DRAFT_4145 and EX28DRAFT_4146 are separated by 88 nucleotides EX28DRAFT_4146 and EX28DRAFT_4147 are separated by 120 nucleotides EX28DRAFT_4147 and EX28DRAFT_4148 overlap by 4 nucleotides
EX28DRAFT_4144: EX28DRAFT_4144 - Intracellular growth attenuator protein IgaA, at 123,992 to 126,127
_4144
EX28DRAFT_4145: EX28DRAFT_4145 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 126,452 to 127,012
_4145
EX28DRAFT_4146: EX28DRAFT_4146 - penicillin-binding protein, 1A family, at 127,101 to 129,653
_4146
EX28DRAFT_4147: EX28DRAFT_4147 - hypothetical protein, at 129,774 to 130,550
_4147
EX28DRAFT_4148: EX28DRAFT_4148 - Tfp pilus assembly protein PilN, at 130,547 to 131,041
_4148
Position (kb)
127
128
129
130 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 126.142 kb on - strand at 126.142 kb on - strand at 126.142 kb on - strand at 126.142 kb on - strand at 126.142 kb on - strand at 126.142 kb on - strand at 126.162 kb on - strand at 126.169 kb on - strand at 126.178 kb on - strand at 126.215 kb on - strand at 126.215 kb on - strand at 126.215 kb on - strand at 126.217 kb on - strand at 126.355 kb on + strand at 126.356 kb on - strand at 126.365 kb on - strand at 126.365 kb on - strand at 126.365 kb on - strand at 126.438 kb on + strand at 126.439 kb on - strand at 126.518 kb on - strand, within EX28DRAFT_4145 at 126.575 kb on - strand, within EX28DRAFT_4145 at 126.575 kb on - strand, within EX28DRAFT_4145 at 127.192 kb on + strand at 127.208 kb on + strand at 127.245 kb on - strand at 127.397 kb on + strand, within EX28DRAFT_4146 at 127.398 kb on - strand, within EX28DRAFT_4146 at 127.404 kb on + strand, within EX28DRAFT_4146 at 127.404 kb on + strand, within EX28DRAFT_4146 at 127.581 kb on + strand, within EX28DRAFT_4146 at 127.581 kb on + strand, within EX28DRAFT_4146 at 127.581 kb on + strand, within EX28DRAFT_4146 at 127.582 kb on - strand, within EX28DRAFT_4146 at 127.645 kb on + strand, within EX28DRAFT_4146 at 127.645 kb on + strand, within EX28DRAFT_4146 at 127.646 kb on - strand, within EX28DRAFT_4146 at 127.785 kb on - strand, within EX28DRAFT_4146 at 127.886 kb on - strand, within EX28DRAFT_4146 at 128.024 kb on - strand, within EX28DRAFT_4146 at 128.109 kb on + strand, within EX28DRAFT_4146 at 128.123 kb on - strand, within EX28DRAFT_4146 at 128.618 kb on - strand, within EX28DRAFT_4146 at 128.639 kb on + strand, within EX28DRAFT_4146 at 128.725 kb on + strand, within EX28DRAFT_4146 at 128.726 kb on - strand, within EX28DRAFT_4146 at 128.726 kb on - strand, within EX28DRAFT_4146 at 128.828 kb on - strand, within EX28DRAFT_4146 at 128.900 kb on - strand, within EX28DRAFT_4146 at 128.953 kb on + strand, within EX28DRAFT_4146 at 128.953 kb on + strand, within EX28DRAFT_4146 at 128.954 kb on - strand, within EX28DRAFT_4146 at 128.954 kb on - strand, within EX28DRAFT_4146 at 128.969 kb on + strand, within EX28DRAFT_4146 at 128.970 kb on - strand, within EX28DRAFT_4146 at 129.107 kb on - strand, within EX28DRAFT_4146 at 129.167 kb on - strand, within EX28DRAFT_4146 at 129.167 kb on - strand, within EX28DRAFT_4146 at 129.236 kb on + strand, within EX28DRAFT_4146 at 129.237 kb on - strand, within EX28DRAFT_4146 at 129.296 kb on + strand, within EX28DRAFT_4146 at 129.297 kb on - strand, within EX28DRAFT_4146 at 129.382 kb on + strand, within EX28DRAFT_4146 at 129.418 kb on - strand at 129.418 kb on - strand at 129.442 kb on - strand at 129.502 kb on + strand at 129.503 kb on - strand at 129.571 kb on + strand at 129.571 kb on + strand at 129.571 kb on + strand at 129.572 kb on - strand at 129.578 kb on - strand at 129.578 kb on - strand at 129.579 kb on + strand at 129.637 kb on + strand at 129.637 kb on + strand at 129.638 kb on - strand at 129.642 kb on + strand at 129.643 kb on - strand at 129.690 kb on + strand at 129.708 kb on + strand at 129.708 kb on + strand at 129.709 kb on - strand at 129.709 kb on - strand at 129.709 kb on - strand at 129.733 kb on + strand at 129.733 kb on + strand at 129.733 kb on + strand at 129.733 kb on + strand at 129.733 kb on + strand at 129.734 kb on - strand at 129.734 kb on - strand at 129.734 kb on - strand at 129.735 kb on + strand at 129.736 kb on - strand at 129.806 kb on + strand at 129.978 kb on + strand, within EX28DRAFT_4147 at 130.157 kb on + strand, within EX28DRAFT_4147 at 130.157 kb on + strand, within EX28DRAFT_4147 at 130.158 kb on - strand, within EX28DRAFT_4147 at 130.506 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 126,142 - -0.8 126,142 - -0.5 126,142 - -0.1 126,142 - +0.3 126,142 - +0.9 126,142 - +0.5 126,162 - -0.2 126,169 - -0.1 126,178 - -0.0 126,215 - -0.4 126,215 - -0.2 126,215 - -0.3 126,217 - +0.5 126,355 + +0.0 126,356 - -0.6 126,365 - +0.6 126,365 - +1.2 126,365 - -0.5 126,438 + -0.6 126,439 - +0.2 126,518 - EX28DRAFT_4145 0.12 -0.8 126,575 - EX28DRAFT_4145 0.22 +0.0 126,575 - EX28DRAFT_4145 0.22 +0.2 127,192 + +0.7 127,208 + +0.7 127,245 - -0.0 127,397 + EX28DRAFT_4146 0.12 +0.3 127,398 - EX28DRAFT_4146 0.12 +0.9 127,404 + EX28DRAFT_4146 0.12 +0.2 127,404 + EX28DRAFT_4146 0.12 +0.7 127,581 + EX28DRAFT_4146 0.19 -0.7 127,581 + EX28DRAFT_4146 0.19 +0.5 127,581 + EX28DRAFT_4146 0.19 +0.4 127,582 - EX28DRAFT_4146 0.19 +0.5 127,645 + EX28DRAFT_4146 0.21 -0.3 127,645 + EX28DRAFT_4146 0.21 -0.1 127,646 - EX28DRAFT_4146 0.21 +0.5 127,785 - EX28DRAFT_4146 0.27 +1.7 127,886 - EX28DRAFT_4146 0.31 -0.6 128,024 - EX28DRAFT_4146 0.36 -0.2 128,109 + EX28DRAFT_4146 0.39 +0.4 128,123 - EX28DRAFT_4146 0.40 +0.7 128,618 - EX28DRAFT_4146 0.59 +0.0 128,639 + EX28DRAFT_4146 0.60 -0.5 128,725 + EX28DRAFT_4146 0.64 -0.3 128,726 - EX28DRAFT_4146 0.64 +0.2 128,726 - EX28DRAFT_4146 0.64 -0.7 128,828 - EX28DRAFT_4146 0.68 -0.2 128,900 - EX28DRAFT_4146 0.70 +0.9 128,953 + EX28DRAFT_4146 0.73 +0.2 128,953 + EX28DRAFT_4146 0.73 -0.0 128,954 - EX28DRAFT_4146 0.73 +0.4 128,954 - EX28DRAFT_4146 0.73 +0.3 128,969 + EX28DRAFT_4146 0.73 -0.4 128,970 - EX28DRAFT_4146 0.73 -0.1 129,107 - EX28DRAFT_4146 0.79 +1.7 129,167 - EX28DRAFT_4146 0.81 -0.1 129,167 - EX28DRAFT_4146 0.81 +0.1 129,236 + EX28DRAFT_4146 0.84 +0.0 129,237 - EX28DRAFT_4146 0.84 +0.7 129,296 + EX28DRAFT_4146 0.86 -0.8 129,297 - EX28DRAFT_4146 0.86 -0.2 129,382 + EX28DRAFT_4146 0.89 -0.6 129,418 - -0.3 129,418 - +1.8 129,442 - +1.1 129,502 + -0.7 129,503 - +0.1 129,571 + -0.3 129,571 + +1.3 129,571 + +0.5 129,572 - +0.1 129,578 - -0.2 129,578 - -0.7 129,579 + +0.1 129,637 + +0.1 129,637 + -0.7 129,638 - +0.4 129,642 + -0.5 129,643 - -0.7 129,690 + +1.0 129,708 + +0.5 129,708 + -3.2 129,709 - -0.0 129,709 - +0.4 129,709 - +0.3 129,733 + -0.5 129,733 + +0.7 129,733 + -0.6 129,733 + -0.7 129,733 + -0.4 129,734 - -0.1 129,734 - -0.9 129,734 - -0.4 129,735 + +1.3 129,736 - +0.5 129,806 + +1.0 129,978 + EX28DRAFT_4147 0.26 -0.1 130,157 + EX28DRAFT_4147 0.49 +0.8 130,157 + EX28DRAFT_4147 0.49 +0.7 130,158 - EX28DRAFT_4147 0.49 -0.0 130,506 + +1.0
Or see this region's nucleotide sequence