Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3951 and EX28DRAFT_3952 overlap by 4 nucleotides EX28DRAFT_3952 and EX28DRAFT_3953 are separated by 100 nucleotides EX28DRAFT_3953 and EX28DRAFT_3954 are separated by 3 nucleotides EX28DRAFT_3954 and EX28DRAFT_3955 are separated by 312 nucleotides EX28DRAFT_3955 and EX28DRAFT_3956 are separated by 21 nucleotides EX28DRAFT_3956 and EX28DRAFT_3957 overlap by 8 nucleotides
EX28DRAFT_3951: EX28DRAFT_3951 - Signal transduction histidine kinase, glucose-6-phosphate specific, at 161,539 to 163,044
_3951
EX28DRAFT_3952: EX28DRAFT_3952 - Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 163,041 to 163,634
_3952
EX28DRAFT_3953: EX28DRAFT_3953 - acetolactate synthase, small subunit (EC 2.2.1.6), at 163,735 to 164,022
_3953
EX28DRAFT_3954: EX28DRAFT_3954 - acetolactate synthase, large subunit (EC 2.2.1.6), at 164,026 to 165,714
_3954
EX28DRAFT_3955: EX28DRAFT_3955 - type VI secretion system effector, Hcp1 family, at 166,027 to 166,506
_3955
EX28DRAFT_3956: EX28DRAFT_3956 - hypothetical protein, at 166,528 to 166,710
_3956
EX28DRAFT_3957: EX28DRAFT_3957 - hypothetical protein, at 166,703 to 167,416
_3957
Position (kb) 164
165
166 Strain fitness (log2 ratio) -2
-1
0
1
2 at 163.171 kb on - strand, within EX28DRAFT_3952 at 163.269 kb on + strand, within EX28DRAFT_3952 at 163.274 kb on - strand, within EX28DRAFT_3952 at 163.406 kb on + strand, within EX28DRAFT_3952 at 163.481 kb on + strand, within EX28DRAFT_3952 at 163.602 kb on - strand at 164.214 kb on + strand, within EX28DRAFT_3954 at 164.235 kb on - strand, within EX28DRAFT_3954 at 164.262 kb on - strand, within EX28DRAFT_3954 at 164.329 kb on + strand, within EX28DRAFT_3954 at 164.556 kb on + strand, within EX28DRAFT_3954 at 164.903 kb on + strand, within EX28DRAFT_3954 at 164.904 kb on - strand, within EX28DRAFT_3954 at 164.904 kb on - strand, within EX28DRAFT_3954 at 164.904 kb on - strand, within EX28DRAFT_3954 at 164.941 kb on + strand, within EX28DRAFT_3954 at 165.066 kb on - strand, within EX28DRAFT_3954 at 165.201 kb on + strand, within EX28DRAFT_3954 at 165.201 kb on + strand, within EX28DRAFT_3954 at 165.201 kb on + strand, within EX28DRAFT_3954 at 165.296 kb on - strand, within EX28DRAFT_3954 at 165.296 kb on - strand, within EX28DRAFT_3954 at 165.296 kb on - strand, within EX28DRAFT_3954 at 165.299 kb on + strand, within EX28DRAFT_3954 at 165.299 kb on + strand, within EX28DRAFT_3954 at 165.300 kb on - strand, within EX28DRAFT_3954 at 165.300 kb on - strand, within EX28DRAFT_3954 at 165.303 kb on + strand, within EX28DRAFT_3954 at 165.304 kb on - strand, within EX28DRAFT_3954 at 165.304 kb on - strand, within EX28DRAFT_3954 at 165.304 kb on - strand, within EX28DRAFT_3954 at 165.332 kb on + strand, within EX28DRAFT_3954 at 165.332 kb on + strand, within EX28DRAFT_3954 at 165.333 kb on - strand, within EX28DRAFT_3954 at 165.587 kb on + strand at 165.588 kb on - strand at 165.588 kb on - strand at 165.588 kb on - strand at 165.953 kb on + strand at 165.956 kb on - strand at 166.059 kb on + strand at 166.061 kb on + strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.152 kb on - strand, within EX28DRAFT_3955 at 166.201 kb on - strand, within EX28DRAFT_3955 at 166.342 kb on + strand, within EX28DRAFT_3955 at 166.342 kb on + strand, within EX28DRAFT_3955 at 166.395 kb on + strand, within EX28DRAFT_3955 at 166.395 kb on + strand, within EX28DRAFT_3955 at 166.600 kb on + strand, within EX28DRAFT_3956 at 166.603 kb on + strand, within EX28DRAFT_3956 at 166.604 kb on - strand, within EX28DRAFT_3956
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 163,171 - EX28DRAFT_3952 0.22 +1.4 163,269 + EX28DRAFT_3952 0.38 -1.3 163,274 - EX28DRAFT_3952 0.39 +0.1 163,406 + EX28DRAFT_3952 0.61 +0.1 163,481 + EX28DRAFT_3952 0.74 -0.3 163,602 - -0.1 164,214 + EX28DRAFT_3954 0.11 -1.3 164,235 - EX28DRAFT_3954 0.12 +0.5 164,262 - EX28DRAFT_3954 0.14 -0.6 164,329 + EX28DRAFT_3954 0.18 -0.8 164,556 + EX28DRAFT_3954 0.31 -0.7 164,903 + EX28DRAFT_3954 0.52 -0.5 164,904 - EX28DRAFT_3954 0.52 -2.1 164,904 - EX28DRAFT_3954 0.52 -0.4 164,904 - EX28DRAFT_3954 0.52 -1.3 164,941 + EX28DRAFT_3954 0.54 +0.3 165,066 - EX28DRAFT_3954 0.62 -0.6 165,201 + EX28DRAFT_3954 0.70 -0.0 165,201 + EX28DRAFT_3954 0.70 -1.1 165,201 + EX28DRAFT_3954 0.70 -2.4 165,296 - EX28DRAFT_3954 0.75 -0.9 165,296 - EX28DRAFT_3954 0.75 -0.3 165,296 - EX28DRAFT_3954 0.75 -1.4 165,299 + EX28DRAFT_3954 0.75 +0.7 165,299 + EX28DRAFT_3954 0.75 -0.6 165,300 - EX28DRAFT_3954 0.75 -1.1 165,300 - EX28DRAFT_3954 0.75 -0.7 165,303 + EX28DRAFT_3954 0.76 -0.5 165,304 - EX28DRAFT_3954 0.76 -1.1 165,304 - EX28DRAFT_3954 0.76 -0.2 165,304 - EX28DRAFT_3954 0.76 -0.2 165,332 + EX28DRAFT_3954 0.77 +0.1 165,332 + EX28DRAFT_3954 0.77 -0.9 165,333 - EX28DRAFT_3954 0.77 -0.8 165,587 + -0.6 165,588 - -1.5 165,588 - -0.9 165,588 - -1.7 165,953 + -0.4 165,956 - +0.1 166,059 + +2.0 166,061 + -1.4 166,062 - -0.2 166,062 - -1.2 166,062 - +0.9 166,062 - +0.3 166,062 - +0.2 166,062 - -2.4 166,062 - +1.0 166,062 - -0.0 166,152 - EX28DRAFT_3955 0.26 -0.0 166,201 - EX28DRAFT_3955 0.36 +0.2 166,342 + EX28DRAFT_3955 0.66 -0.3 166,342 + EX28DRAFT_3955 0.66 +0.2 166,395 + EX28DRAFT_3955 0.77 -0.2 166,395 + EX28DRAFT_3955 0.77 +0.3 166,600 + EX28DRAFT_3956 0.39 -0.9 166,603 + EX28DRAFT_3956 0.41 +0.3 166,604 - EX28DRAFT_3956 0.42 +0.2
Or see this region's nucleotide sequence