Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3669 and EX28DRAFT_3670 overlap by 1 nucleotides EX28DRAFT_3670 and EX28DRAFT_3671 are separated by 43 nucleotides EX28DRAFT_3671 and EX28DRAFT_3672 are separated by 574 nucleotides
EX28DRAFT_3669: EX28DRAFT_3669 - 3-isopropylmalate dehydrogenase, at 335,795 to 336,886
_3669
EX28DRAFT_3670: EX28DRAFT_3670 - 2-isopropylmalate synthase (EC 2.3.3.13), at 336,886 to 338,457
_3670
EX28DRAFT_3671: EX28DRAFT_3671 - RNA, at 338,501 to 338,649
_3671
EX28DRAFT_3672: EX28DRAFT_3672 - Transcriptional regulator, at 339,224 to 340,186
_3672
Position (kb) 336
337
338
339 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.214 kb on - strand, within EX28DRAFT_3669 at 336.323 kb on - strand, within EX28DRAFT_3669 at 336.323 kb on - strand, within EX28DRAFT_3669 at 336.388 kb on - strand, within EX28DRAFT_3669 at 336.388 kb on - strand, within EX28DRAFT_3669 at 336.388 kb on - strand, within EX28DRAFT_3669 at 336.760 kb on - strand, within EX28DRAFT_3669 at 336.869 kb on - strand at 336.874 kb on - strand at 337.112 kb on + strand, within EX28DRAFT_3670 at 337.160 kb on + strand, within EX28DRAFT_3670 at 337.161 kb on - strand, within EX28DRAFT_3670 at 337.161 kb on - strand, within EX28DRAFT_3670 at 337.274 kb on + strand, within EX28DRAFT_3670 at 337.315 kb on - strand, within EX28DRAFT_3670 at 337.419 kb on + strand, within EX28DRAFT_3670 at 337.419 kb on + strand, within EX28DRAFT_3670 at 337.421 kb on + strand, within EX28DRAFT_3670 at 337.421 kb on + strand, within EX28DRAFT_3670 at 337.421 kb on + strand, within EX28DRAFT_3670 at 337.421 kb on + strand, within EX28DRAFT_3670 at 337.438 kb on - strand, within EX28DRAFT_3670 at 337.486 kb on + strand, within EX28DRAFT_3670 at 337.486 kb on + strand, within EX28DRAFT_3670 at 337.695 kb on + strand, within EX28DRAFT_3670 at 337.762 kb on + strand, within EX28DRAFT_3670 at 337.765 kb on - strand, within EX28DRAFT_3670 at 337.821 kb on + strand, within EX28DRAFT_3670 at 337.864 kb on - strand, within EX28DRAFT_3670 at 337.937 kb on + strand, within EX28DRAFT_3670 at 338.064 kb on - strand, within EX28DRAFT_3670 at 338.087 kb on + strand, within EX28DRAFT_3670 at 338.087 kb on + strand, within EX28DRAFT_3670 at 338.087 kb on + strand, within EX28DRAFT_3670 at 338.346 kb on + strand at 338.427 kb on + strand at 338.427 kb on + strand at 338.427 kb on + strand at 338.428 kb on - strand at 338.463 kb on - strand at 338.500 kb on + strand at 338.501 kb on - strand at 338.537 kb on - strand, within EX28DRAFT_3671 at 338.600 kb on + strand, within EX28DRAFT_3671 at 338.603 kb on + strand, within EX28DRAFT_3671 at 338.604 kb on - strand, within EX28DRAFT_3671 at 338.604 kb on - strand, within EX28DRAFT_3671 at 338.604 kb on - strand, within EX28DRAFT_3671 at 338.604 kb on - strand, within EX28DRAFT_3671 at 338.659 kb on + strand at 338.668 kb on - strand at 338.755 kb on - strand at 338.832 kb on - strand at 338.904 kb on + strand at 338.926 kb on + strand at 339.037 kb on + strand at 339.083 kb on + strand at 339.194 kb on + strand at 339.259 kb on + strand at 339.298 kb on - strand at 339.355 kb on + strand, within EX28DRAFT_3672
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 336,213 + EX28DRAFT_3669 0.38 -3.1 336,213 + EX28DRAFT_3669 0.38 -1.6 336,213 + EX28DRAFT_3669 0.38 -2.4 336,213 + EX28DRAFT_3669 0.38 -3.0 336,213 + EX28DRAFT_3669 0.38 -2.8 336,214 - EX28DRAFT_3669 0.38 -3.8 336,323 - EX28DRAFT_3669 0.48 -4.1 336,323 - EX28DRAFT_3669 0.48 -3.2 336,388 - EX28DRAFT_3669 0.54 -5.0 336,388 - EX28DRAFT_3669 0.54 -0.5 336,388 - EX28DRAFT_3669 0.54 -3.2 336,760 - EX28DRAFT_3669 0.88 -3.1 336,869 - -3.8 336,874 - -3.4 337,112 + EX28DRAFT_3670 0.14 -3.3 337,160 + EX28DRAFT_3670 0.17 -3.9 337,161 - EX28DRAFT_3670 0.17 -1.4 337,161 - EX28DRAFT_3670 0.17 -1.0 337,274 + EX28DRAFT_3670 0.25 +0.5 337,315 - EX28DRAFT_3670 0.27 -0.5 337,419 + EX28DRAFT_3670 0.34 -4.8 337,419 + EX28DRAFT_3670 0.34 -4.7 337,421 + EX28DRAFT_3670 0.34 -3.7 337,421 + EX28DRAFT_3670 0.34 -2.3 337,421 + EX28DRAFT_3670 0.34 -5.2 337,421 + EX28DRAFT_3670 0.34 -3.9 337,438 - EX28DRAFT_3670 0.35 -2.9 337,486 + EX28DRAFT_3670 0.38 -1.5 337,486 + EX28DRAFT_3670 0.38 -4.3 337,695 + EX28DRAFT_3670 0.51 -3.4 337,762 + EX28DRAFT_3670 0.56 -2.7 337,765 - EX28DRAFT_3670 0.56 -4.2 337,821 + EX28DRAFT_3670 0.59 -2.8 337,864 - EX28DRAFT_3670 0.62 -3.1 337,937 + EX28DRAFT_3670 0.67 -3.3 338,064 - EX28DRAFT_3670 0.75 -3.5 338,087 + EX28DRAFT_3670 0.76 -3.8 338,087 + EX28DRAFT_3670 0.76 +0.9 338,087 + EX28DRAFT_3670 0.76 -3.3 338,346 + -2.6 338,427 + -2.1 338,427 + -1.5 338,427 + -1.3 338,428 - -2.5 338,463 - -2.8 338,500 + -1.2 338,501 - -1.1 338,537 - EX28DRAFT_3671 0.24 -1.9 338,600 + EX28DRAFT_3671 0.66 -4.7 338,603 + EX28DRAFT_3671 0.68 -3.0 338,604 - EX28DRAFT_3671 0.69 -2.8 338,604 - EX28DRAFT_3671 0.69 -0.9 338,604 - EX28DRAFT_3671 0.69 -1.8 338,604 - EX28DRAFT_3671 0.69 -2.4 338,659 + -3.7 338,668 - -2.2 338,755 - +0.1 338,832 - +0.0 338,904 + +0.8 338,926 + -0.7 339,037 + -0.9 339,083 + -0.2 339,194 + -0.1 339,259 + -0.1 339,298 - -0.7 339,355 + EX28DRAFT_3672 0.14 -1.6
Or see this region's nucleotide sequence