Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3381 and EX28DRAFT_3382 are separated by 148 nucleotides EX28DRAFT_3382 and EX28DRAFT_3383 are separated by 158 nucleotides EX28DRAFT_3383 and EX28DRAFT_3384 are separated by 169 nucleotides EX28DRAFT_3384 and EX28DRAFT_3385 are separated by 341 nucleotides
EX28DRAFT_3381: EX28DRAFT_3381 - Protein of unknown function (DUF1471), at 52,428 to 52,703
_3381
EX28DRAFT_3382: EX28DRAFT_3382 - Protein of unknown function (DUF1471), at 52,852 to 53,160
_3382
EX28DRAFT_3383: EX28DRAFT_3383 - Alpha/beta hydrolase family, at 53,319 to 54,041
_3383
EX28DRAFT_3384: EX28DRAFT_3384 - Protein of unknown function (DUF1471), at 54,211 to 54,486
_3384
EX28DRAFT_3385: EX28DRAFT_3385 - ribosomal protein S6, at 54,828 to 55,223
_3385
Position (kb) 53
54
55 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 52.429 kb on + strand at 52.527 kb on + strand, within EX28DRAFT_3381 at 52.572 kb on - strand, within EX28DRAFT_3381 at 52.722 kb on + strand at 52.722 kb on + strand at 52.723 kb on - strand at 52.756 kb on + strand at 52.790 kb on - strand at 52.811 kb on - strand at 52.811 kb on - strand at 52.828 kb on + strand at 52.854 kb on - strand at 52.857 kb on + strand at 52.857 kb on + strand at 52.858 kb on - strand at 52.858 kb on - strand at 52.858 kb on - strand at 52.894 kb on + strand, within EX28DRAFT_3382 at 52.926 kb on - strand, within EX28DRAFT_3382 at 52.926 kb on - strand, within EX28DRAFT_3382 at 52.929 kb on - strand, within EX28DRAFT_3382 at 53.024 kb on - strand, within EX28DRAFT_3382 at 53.154 kb on + strand at 53.160 kb on + strand at 53.160 kb on + strand at 53.160 kb on + strand at 53.161 kb on - strand at 53.181 kb on + strand at 53.181 kb on + strand at 53.182 kb on - strand at 53.186 kb on + strand at 53.186 kb on + strand at 53.186 kb on + strand at 53.186 kb on + strand at 53.187 kb on - strand at 53.242 kb on - strand at 53.242 kb on - strand at 53.272 kb on - strand at 53.289 kb on + strand at 53.298 kb on + strand at 53.298 kb on + strand at 53.336 kb on - strand at 53.614 kb on - strand, within EX28DRAFT_3383 at 54.065 kb on - strand at 54.109 kb on - strand at 54.112 kb on - strand at 54.112 kb on - strand at 54.112 kb on - strand at 54.163 kb on + strand at 54.163 kb on + strand at 54.163 kb on + strand at 54.164 kb on - strand at 54.164 kb on - strand at 54.164 kb on - strand at 54.164 kb on - strand at 54.169 kb on + strand at 54.218 kb on + strand at 54.222 kb on + strand at 54.223 kb on - strand at 54.284 kb on + strand, within EX28DRAFT_3384 at 54.284 kb on + strand, within EX28DRAFT_3384 at 54.285 kb on - strand, within EX28DRAFT_3384 at 54.285 kb on - strand, within EX28DRAFT_3384 at 54.352 kb on - strand, within EX28DRAFT_3384 at 54.363 kb on - strand, within EX28DRAFT_3384 at 54.415 kb on + strand, within EX28DRAFT_3384 at 54.416 kb on - strand, within EX28DRAFT_3384 at 54.490 kb on - strand at 54.504 kb on + strand at 54.505 kb on - strand at 54.557 kb on - strand at 54.563 kb on + strand at 54.563 kb on + strand at 54.645 kb on - strand at 54.658 kb on - strand at 54.658 kb on - strand at 54.684 kb on + strand at 54.685 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 52,429 + +0.2 52,527 + EX28DRAFT_3381 0.36 -0.4 52,572 - EX28DRAFT_3381 0.52 -1.7 52,722 + -0.1 52,722 + +0.4 52,723 - -0.4 52,756 + -1.3 52,790 - -3.3 52,811 - +0.3 52,811 - -0.2 52,828 + +0.5 52,854 - -0.4 52,857 + +0.5 52,857 + -1.2 52,858 - +0.1 52,858 - +0.1 52,858 - -1.6 52,894 + EX28DRAFT_3382 0.14 +0.0 52,926 - EX28DRAFT_3382 0.24 +1.6 52,926 - EX28DRAFT_3382 0.24 +0.1 52,929 - EX28DRAFT_3382 0.25 -0.4 53,024 - EX28DRAFT_3382 0.56 +0.2 53,154 + -0.2 53,160 + -0.7 53,160 + +0.3 53,160 + +0.1 53,161 - +0.3 53,181 + +0.8 53,181 + -1.0 53,182 - -1.4 53,186 + -1.7 53,186 + -1.6 53,186 + -1.3 53,186 + -1.1 53,187 - -1.0 53,242 - -0.2 53,242 - -0.2 53,272 - +0.6 53,289 + +2.6 53,298 + +0.7 53,298 + -1.4 53,336 - +0.0 53,614 - EX28DRAFT_3383 0.41 -0.7 54,065 - -0.2 54,109 - -0.3 54,112 - +0.6 54,112 - -0.4 54,112 - -0.1 54,163 + +0.6 54,163 + +0.2 54,163 + -0.2 54,164 - -0.4 54,164 - +0.5 54,164 - +2.2 54,164 - -0.1 54,169 + -0.5 54,218 + +0.1 54,222 + -0.3 54,223 - -0.2 54,284 + EX28DRAFT_3384 0.26 -0.0 54,284 + EX28DRAFT_3384 0.26 -0.2 54,285 - EX28DRAFT_3384 0.27 -0.9 54,285 - EX28DRAFT_3384 0.27 +0.8 54,352 - EX28DRAFT_3384 0.51 +0.1 54,363 - EX28DRAFT_3384 0.55 -0.1 54,415 + EX28DRAFT_3384 0.74 +0.1 54,416 - EX28DRAFT_3384 0.74 +0.5 54,490 - -1.1 54,504 + +0.2 54,505 - -0.0 54,557 - -0.1 54,563 + -0.9 54,563 + -0.0 54,645 - +0.3 54,658 - -1.1 54,658 - -1.6 54,684 + -0.3 54,685 - +0.0
Or see this region's nucleotide sequence