Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2371 and EX28DRAFT_2372 overlap by 1 nucleotides EX28DRAFT_2372 and EX28DRAFT_2373 are separated by 10 nucleotides EX28DRAFT_2373 and EX28DRAFT_2374 are separated by 9 nucleotides
EX28DRAFT_2371: EX28DRAFT_2371 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, at 2,390,946 to 2,391,698
_2371
EX28DRAFT_2372: EX28DRAFT_2372 - Isochorismate hydrolase, at 2,391,698 to 2,392,552
_2372
EX28DRAFT_2373: EX28DRAFT_2373 - 2,3-dihydroxybenzoate-AMP ligase, at 2,392,563 to 2,394,173
_2373
EX28DRAFT_2374: EX28DRAFT_2374 - isochorismate synthase (EC 5.4.4.2), at 2,394,183 to 2,395,358
_2374
Position (kb)
2392
2393
2394
2395 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2391.677 kb on + strand at 2391.978 kb on + strand, within EX28DRAFT_2372 at 2391.979 kb on - strand, within EX28DRAFT_2372 at 2391.979 kb on - strand, within EX28DRAFT_2372 at 2391.979 kb on - strand, within EX28DRAFT_2372 at 2392.057 kb on + strand, within EX28DRAFT_2372 at 2392.098 kb on + strand, within EX28DRAFT_2372 at 2392.100 kb on + strand, within EX28DRAFT_2372 at 2392.101 kb on - strand, within EX28DRAFT_2372 at 2392.339 kb on + strand, within EX28DRAFT_2372 at 2392.431 kb on + strand, within EX28DRAFT_2372 at 2392.493 kb on - strand at 2392.903 kb on + strand, within EX28DRAFT_2373 at 2392.943 kb on - strand, within EX28DRAFT_2373 at 2393.152 kb on + strand, within EX28DRAFT_2373 at 2393.153 kb on - strand, within EX28DRAFT_2373 at 2393.277 kb on - strand, within EX28DRAFT_2373 at 2393.468 kb on - strand, within EX28DRAFT_2373 at 2393.468 kb on - strand, within EX28DRAFT_2373 at 2393.468 kb on - strand, within EX28DRAFT_2373 at 2393.472 kb on - strand, within EX28DRAFT_2373 at 2393.472 kb on - strand, within EX28DRAFT_2373 at 2393.497 kb on + strand, within EX28DRAFT_2373 at 2393.548 kb on + strand, within EX28DRAFT_2373 at 2393.549 kb on - strand, within EX28DRAFT_2373 at 2393.915 kb on - strand, within EX28DRAFT_2373 at 2393.915 kb on - strand, within EX28DRAFT_2373 at 2394.158 kb on + strand at 2394.668 kb on + strand, within EX28DRAFT_2374 at 2394.690 kb on + strand, within EX28DRAFT_2374 at 2394.690 kb on + strand, within EX28DRAFT_2374 at 2394.690 kb on + strand, within EX28DRAFT_2374 at 2394.691 kb on - strand, within EX28DRAFT_2374 at 2395.037 kb on - strand, within EX28DRAFT_2374
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,391,677 + +0.1 2,391,978 + EX28DRAFT_2372 0.33 -0.1 2,391,979 - EX28DRAFT_2372 0.33 +0.1 2,391,979 - EX28DRAFT_2372 0.33 +0.0 2,391,979 - EX28DRAFT_2372 0.33 -0.3 2,392,057 + EX28DRAFT_2372 0.42 -1.1 2,392,098 + EX28DRAFT_2372 0.47 -0.7 2,392,100 + EX28DRAFT_2372 0.47 +0.2 2,392,101 - EX28DRAFT_2372 0.47 -0.3 2,392,339 + EX28DRAFT_2372 0.75 -0.5 2,392,431 + EX28DRAFT_2372 0.86 -0.1 2,392,493 - +2.2 2,392,903 + EX28DRAFT_2373 0.21 -0.1 2,392,943 - EX28DRAFT_2373 0.24 -1.5 2,393,152 + EX28DRAFT_2373 0.37 -0.9 2,393,153 - EX28DRAFT_2373 0.37 +0.4 2,393,277 - EX28DRAFT_2373 0.44 +0.4 2,393,468 - EX28DRAFT_2373 0.56 -2.6 2,393,468 - EX28DRAFT_2373 0.56 -0.7 2,393,468 - EX28DRAFT_2373 0.56 -0.1 2,393,472 - EX28DRAFT_2373 0.56 -0.7 2,393,472 - EX28DRAFT_2373 0.56 -0.4 2,393,497 + EX28DRAFT_2373 0.58 -0.7 2,393,548 + EX28DRAFT_2373 0.61 +0.1 2,393,549 - EX28DRAFT_2373 0.61 +0.3 2,393,915 - EX28DRAFT_2373 0.84 -0.4 2,393,915 - EX28DRAFT_2373 0.84 +1.2 2,394,158 + +0.1 2,394,668 + EX28DRAFT_2374 0.41 -1.5 2,394,690 + EX28DRAFT_2374 0.43 +0.3 2,394,690 + EX28DRAFT_2374 0.43 -0.2 2,394,690 + EX28DRAFT_2374 0.43 +2.1 2,394,691 - EX28DRAFT_2374 0.43 +0.3 2,395,037 - EX28DRAFT_2374 0.73 +0.0
Or see this region's nucleotide sequence