Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1940 and EX28DRAFT_1941 are separated by 10 nucleotides EX28DRAFT_1941 and EX28DRAFT_1942 overlap by 4 nucleotides EX28DRAFT_1942 and EX28DRAFT_1943 overlap by 4 nucleotides EX28DRAFT_1943 and EX28DRAFT_1944 are separated by 69 nucleotides
EX28DRAFT_1940: EX28DRAFT_1940 - Gamma-glutamyltransferase, at 1,945,766 to 1,947,349
_1940
EX28DRAFT_1941: EX28DRAFT_1941 - Protein of unknown function (DUF4089), at 1,947,360 to 1,947,548
_1941
EX28DRAFT_1942: EX28DRAFT_1942 - amidohydrolase, AtzE family, at 1,947,545 to 1,948,942
_1942
EX28DRAFT_1943: EX28DRAFT_1943 - Protein of unknown function (DUF3225), at 1,948,939 to 1,949,325
_1943
EX28DRAFT_1944: EX28DRAFT_1944 - putative urate catabolism protein, at 1,949,395 to 1,950,327
_1944
Position (kb) 1947
1948
1949 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1946.551 kb on - strand, within EX28DRAFT_1940 at 1946.576 kb on - strand, within EX28DRAFT_1940 at 1946.842 kb on + strand, within EX28DRAFT_1940 at 1946.843 kb on - strand, within EX28DRAFT_1940 at 1946.846 kb on + strand, within EX28DRAFT_1940 at 1947.099 kb on + strand, within EX28DRAFT_1940 at 1947.099 kb on + strand, within EX28DRAFT_1940 at 1947.234 kb on + strand at 1947.234 kb on + strand at 1947.234 kb on + strand at 1947.473 kb on - strand, within EX28DRAFT_1941 at 1947.535 kb on + strand at 1947.536 kb on - strand at 1947.537 kb on + strand at 1947.537 kb on + strand at 1947.537 kb on + strand at 1947.538 kb on - strand at 1947.538 kb on - strand at 1947.565 kb on + strand at 1947.916 kb on + strand, within EX28DRAFT_1942 at 1947.916 kb on + strand, within EX28DRAFT_1942 at 1947.917 kb on - strand, within EX28DRAFT_1942 at 1948.465 kb on + strand, within EX28DRAFT_1942 at 1948.587 kb on - strand, within EX28DRAFT_1942 at 1948.587 kb on - strand, within EX28DRAFT_1942 at 1948.780 kb on - strand, within EX28DRAFT_1942 at 1949.000 kb on - strand, within EX28DRAFT_1943 at 1949.396 kb on + strand at 1949.396 kb on + strand at 1949.397 kb on - strand at 1949.397 kb on - strand at 1949.478 kb on + strand at 1949.479 kb on - strand at 1949.479 kb on - strand at 1949.479 kb on - strand at 1949.480 kb on + strand at 1949.609 kb on + strand, within EX28DRAFT_1944 at 1949.610 kb on - strand, within EX28DRAFT_1944 at 1949.613 kb on + strand, within EX28DRAFT_1944 at 1949.613 kb on + strand, within EX28DRAFT_1944 at 1949.613 kb on + strand, within EX28DRAFT_1944 at 1949.614 kb on - strand, within EX28DRAFT_1944 at 1949.615 kb on + strand, within EX28DRAFT_1944 at 1949.615 kb on + strand, within EX28DRAFT_1944 at 1949.615 kb on + strand, within EX28DRAFT_1944 at 1949.615 kb on + strand, within EX28DRAFT_1944 at 1949.616 kb on - strand, within EX28DRAFT_1944 at 1949.616 kb on - strand, within EX28DRAFT_1944 at 1949.616 kb on - strand, within EX28DRAFT_1944 at 1949.759 kb on + strand, within EX28DRAFT_1944 at 1949.832 kb on - strand, within EX28DRAFT_1944 at 1949.925 kb on - strand, within EX28DRAFT_1944
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,946,551 - EX28DRAFT_1940 0.50 +0.4 1,946,576 - EX28DRAFT_1940 0.51 -0.3 1,946,842 + EX28DRAFT_1940 0.68 -3.3 1,946,843 - EX28DRAFT_1940 0.68 -0.3 1,946,846 + EX28DRAFT_1940 0.68 +0.4 1,947,099 + EX28DRAFT_1940 0.84 +0.6 1,947,099 + EX28DRAFT_1940 0.84 -0.6 1,947,234 + +1.0 1,947,234 + -0.4 1,947,234 + +1.0 1,947,473 - EX28DRAFT_1941 0.60 -1.1 1,947,535 + +0.2 1,947,536 - +1.9 1,947,537 + -0.5 1,947,537 + -0.8 1,947,537 + -0.1 1,947,538 - -0.2 1,947,538 - -2.7 1,947,565 + +0.8 1,947,916 + EX28DRAFT_1942 0.27 -0.6 1,947,916 + EX28DRAFT_1942 0.27 +3.3 1,947,917 - EX28DRAFT_1942 0.27 +0.4 1,948,465 + EX28DRAFT_1942 0.66 -0.5 1,948,587 - EX28DRAFT_1942 0.75 +1.7 1,948,587 - EX28DRAFT_1942 0.75 -0.6 1,948,780 - EX28DRAFT_1942 0.88 +2.4 1,949,000 - EX28DRAFT_1943 0.16 -0.1 1,949,396 + +0.5 1,949,396 + -0.1 1,949,397 - +1.1 1,949,397 - -2.4 1,949,478 + -0.5 1,949,479 - -0.2 1,949,479 - -1.1 1,949,479 - +1.1 1,949,480 + -0.1 1,949,609 + EX28DRAFT_1944 0.23 -1.1 1,949,610 - EX28DRAFT_1944 0.23 +0.3 1,949,613 + EX28DRAFT_1944 0.23 +0.3 1,949,613 + EX28DRAFT_1944 0.23 -0.7 1,949,613 + EX28DRAFT_1944 0.23 +0.8 1,949,614 - EX28DRAFT_1944 0.23 -0.4 1,949,615 + EX28DRAFT_1944 0.24 +1.2 1,949,615 + EX28DRAFT_1944 0.24 -0.4 1,949,615 + EX28DRAFT_1944 0.24 -0.1 1,949,615 + EX28DRAFT_1944 0.24 -1.3 1,949,616 - EX28DRAFT_1944 0.24 -0.1 1,949,616 - EX28DRAFT_1944 0.24 -0.2 1,949,616 - EX28DRAFT_1944 0.24 +0.6 1,949,759 + EX28DRAFT_1944 0.39 +0.6 1,949,832 - EX28DRAFT_1944 0.47 +0.2 1,949,925 - EX28DRAFT_1944 0.57 -0.0
Or see this region's nucleotide sequence