Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1823 and EX28DRAFT_1824 are separated by 5 nucleotides EX28DRAFT_1824 and EX28DRAFT_1825 overlap by 20 nucleotides EX28DRAFT_1825 and EX28DRAFT_1826 are separated by 10 nucleotides EX28DRAFT_1826 and EX28DRAFT_1827 are separated by 1 nucleotides EX28DRAFT_1827 and EX28DRAFT_1828 are separated by 247 nucleotides
EX28DRAFT_1823: EX28DRAFT_1823 - Beta-glucosidase-related glycosidases, at 1,841,700 to 1,842,731
_1823
EX28DRAFT_1824: EX28DRAFT_1824 - Predicted choline kinase involved in LPS biosynthesis, at 1,842,737 to 1,843,561
_1824
EX28DRAFT_1825: EX28DRAFT_1825 - uncharacterized proteobacterial lipoprotein, at 1,843,542 to 1,844,189
_1825
EX28DRAFT_1826: EX28DRAFT_1826 - Predicted periplasmic lipoprotein, at 1,844,200 to 1,844,574
_1826
EX28DRAFT_1827: EX28DRAFT_1827 - Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases, at 1,844,576 to 1,844,935
_1827
EX28DRAFT_1828: EX28DRAFT_1828 - PTS system, glucose-specific IIBC component, at 1,845,183 to 1,846,616
_1828
Position (kb)
1843
1844
1845 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1842.619 kb on + strand, within EX28DRAFT_1823 at 1842.746 kb on - strand at 1842.753 kb on - strand at 1842.895 kb on + strand, within EX28DRAFT_1824 at 1842.897 kb on + strand, within EX28DRAFT_1824 at 1842.897 kb on + strand, within EX28DRAFT_1824 at 1842.898 kb on - strand, within EX28DRAFT_1824 at 1842.997 kb on - strand, within EX28DRAFT_1824 at 1843.030 kb on + strand, within EX28DRAFT_1824 at 1843.031 kb on - strand, within EX28DRAFT_1824 at 1843.039 kb on + strand, within EX28DRAFT_1824 at 1843.039 kb on + strand, within EX28DRAFT_1824 at 1843.040 kb on - strand, within EX28DRAFT_1824 at 1843.040 kb on - strand, within EX28DRAFT_1824 at 1843.061 kb on - strand, within EX28DRAFT_1824 at 1843.061 kb on - strand, within EX28DRAFT_1824 at 1843.131 kb on - strand, within EX28DRAFT_1824 at 1843.138 kb on + strand, within EX28DRAFT_1824 at 1843.139 kb on - strand, within EX28DRAFT_1824 at 1843.173 kb on - strand, within EX28DRAFT_1824 at 1843.173 kb on - strand, within EX28DRAFT_1824 at 1843.230 kb on + strand, within EX28DRAFT_1824 at 1843.273 kb on + strand, within EX28DRAFT_1824 at 1843.291 kb on - strand, within EX28DRAFT_1824 at 1843.445 kb on + strand, within EX28DRAFT_1824 at 1843.445 kb on + strand, within EX28DRAFT_1824 at 1843.445 kb on + strand, within EX28DRAFT_1824 at 1843.445 kb on + strand, within EX28DRAFT_1824 at 1843.446 kb on - strand, within EX28DRAFT_1824 at 1843.447 kb on + strand, within EX28DRAFT_1824 at 1843.448 kb on - strand, within EX28DRAFT_1824 at 1843.512 kb on + strand at 1843.513 kb on - strand at 1843.566 kb on - strand at 1843.660 kb on + strand, within EX28DRAFT_1825 at 1843.660 kb on + strand, within EX28DRAFT_1825 at 1843.676 kb on + strand, within EX28DRAFT_1825 at 1843.778 kb on + strand, within EX28DRAFT_1825 at 1844.207 kb on + strand at 1844.226 kb on + strand at 1844.227 kb on - strand at 1844.241 kb on - strand, within EX28DRAFT_1826 at 1844.241 kb on - strand, within EX28DRAFT_1826 at 1844.329 kb on + strand, within EX28DRAFT_1826 at 1844.330 kb on - strand, within EX28DRAFT_1826 at 1844.545 kb on + strand at 1844.587 kb on + strand at 1844.587 kb on + strand at 1844.588 kb on - strand at 1844.588 kb on - strand at 1844.588 kb on - strand at 1844.638 kb on + strand, within EX28DRAFT_1827 at 1844.743 kb on + strand, within EX28DRAFT_1827 at 1844.743 kb on + strand, within EX28DRAFT_1827 at 1844.743 kb on + strand, within EX28DRAFT_1827 at 1844.784 kb on - strand, within EX28DRAFT_1827 at 1844.784 kb on - strand, within EX28DRAFT_1827 at 1844.874 kb on + strand, within EX28DRAFT_1827 at 1844.980 kb on - strand at 1845.020 kb on + strand at 1845.137 kb on + strand at 1845.164 kb on - strand at 1845.164 kb on - strand at 1845.184 kb on + strand at 1845.184 kb on + strand at 1845.184 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,842,619 + EX28DRAFT_1823 0.89 -0.3 1,842,746 - +0.2 1,842,753 - -0.0 1,842,895 + EX28DRAFT_1824 0.19 -0.6 1,842,897 + EX28DRAFT_1824 0.19 +0.0 1,842,897 + EX28DRAFT_1824 0.19 +0.4 1,842,898 - EX28DRAFT_1824 0.20 +0.1 1,842,997 - EX28DRAFT_1824 0.32 +0.4 1,843,030 + EX28DRAFT_1824 0.36 +0.1 1,843,031 - EX28DRAFT_1824 0.36 +0.5 1,843,039 + EX28DRAFT_1824 0.37 +0.4 1,843,039 + EX28DRAFT_1824 0.37 -0.1 1,843,040 - EX28DRAFT_1824 0.37 -0.6 1,843,040 - EX28DRAFT_1824 0.37 -0.5 1,843,061 - EX28DRAFT_1824 0.39 +1.6 1,843,061 - EX28DRAFT_1824 0.39 +0.9 1,843,131 - EX28DRAFT_1824 0.48 -1.1 1,843,138 + EX28DRAFT_1824 0.49 -1.2 1,843,139 - EX28DRAFT_1824 0.49 +0.2 1,843,173 - EX28DRAFT_1824 0.53 +0.6 1,843,173 - EX28DRAFT_1824 0.53 +0.1 1,843,230 + EX28DRAFT_1824 0.60 -0.5 1,843,273 + EX28DRAFT_1824 0.65 -0.3 1,843,291 - EX28DRAFT_1824 0.67 -0.9 1,843,445 + EX28DRAFT_1824 0.86 +0.4 1,843,445 + EX28DRAFT_1824 0.86 +1.3 1,843,445 + EX28DRAFT_1824 0.86 +0.0 1,843,445 + EX28DRAFT_1824 0.86 -0.4 1,843,446 - EX28DRAFT_1824 0.86 +0.0 1,843,447 + EX28DRAFT_1824 0.86 -0.7 1,843,448 - EX28DRAFT_1824 0.86 -0.0 1,843,512 + -0.0 1,843,513 - +1.4 1,843,566 - -1.0 1,843,660 + EX28DRAFT_1825 0.18 -2.3 1,843,660 + EX28DRAFT_1825 0.18 -2.3 1,843,676 + EX28DRAFT_1825 0.21 -0.6 1,843,778 + EX28DRAFT_1825 0.36 +0.2 1,844,207 + +0.4 1,844,226 + +0.1 1,844,227 - -0.1 1,844,241 - EX28DRAFT_1826 0.11 -0.4 1,844,241 - EX28DRAFT_1826 0.11 +0.3 1,844,329 + EX28DRAFT_1826 0.34 -2.3 1,844,330 - EX28DRAFT_1826 0.35 -0.1 1,844,545 + -0.7 1,844,587 + -0.7 1,844,587 + -1.2 1,844,588 - +0.5 1,844,588 - -0.2 1,844,588 - +0.0 1,844,638 + EX28DRAFT_1827 0.17 +1.8 1,844,743 + EX28DRAFT_1827 0.46 +1.0 1,844,743 + EX28DRAFT_1827 0.46 +0.3 1,844,743 + EX28DRAFT_1827 0.46 -0.2 1,844,784 - EX28DRAFT_1827 0.58 +0.4 1,844,784 - EX28DRAFT_1827 0.58 -0.6 1,844,874 + EX28DRAFT_1827 0.83 +0.3 1,844,980 - -1.5 1,845,020 + -0.3 1,845,137 + +0.7 1,845,164 - -0.5 1,845,164 - -0.4 1,845,184 + +1.6 1,845,184 + -0.3 1,845,184 + +0.0
Or see this region's nucleotide sequence