Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1618 and EX28DRAFT_1619 are separated by 52 nucleotides EX28DRAFT_1619 and EX28DRAFT_1620 are separated by 231 nucleotides EX28DRAFT_1620 and EX28DRAFT_1621 are separated by 231 nucleotides EX28DRAFT_1621 and EX28DRAFT_1622 are separated by 86 nucleotides
EX28DRAFT_1618: EX28DRAFT_1618 - adenosine deaminase, at 1,637,243 to 1,638,244
_1618
EX28DRAFT_1619: EX28DRAFT_1619 - Predicted dehydrogenases and related proteins, at 1,638,297 to 1,639,337
_1619
EX28DRAFT_1620: EX28DRAFT_1620 - hypothetical protein, at 1,639,569 to 1,639,706
_1620
EX28DRAFT_1621: EX28DRAFT_1621 - Haemolysin expression modulating protein, at 1,639,938 to 1,640,153
_1621
EX28DRAFT_1622: EX28DRAFT_1622 - Protein of unknown function (DUF2569), at 1,640,240 to 1,640,680
_1622
Position (kb)
1638
1639
1640 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1637.340 kb on + strand at 1637.383 kb on - strand, within EX28DRAFT_1618 at 1637.522 kb on + strand, within EX28DRAFT_1618 at 1637.522 kb on + strand, within EX28DRAFT_1618 at 1637.623 kb on + strand, within EX28DRAFT_1618 at 1637.624 kb on - strand, within EX28DRAFT_1618 at 1637.659 kb on + strand, within EX28DRAFT_1618 at 1637.660 kb on - strand, within EX28DRAFT_1618 at 1637.669 kb on - strand, within EX28DRAFT_1618 at 1637.986 kb on + strand, within EX28DRAFT_1618 at 1637.987 kb on - strand, within EX28DRAFT_1618 at 1638.272 kb on - strand at 1638.433 kb on + strand, within EX28DRAFT_1619 at 1638.434 kb on - strand, within EX28DRAFT_1619 at 1638.434 kb on - strand, within EX28DRAFT_1619 at 1638.434 kb on - strand, within EX28DRAFT_1619 at 1638.518 kb on + strand, within EX28DRAFT_1619 at 1638.524 kb on - strand, within EX28DRAFT_1619 at 1638.621 kb on - strand, within EX28DRAFT_1619 at 1638.706 kb on + strand, within EX28DRAFT_1619 at 1638.706 kb on + strand, within EX28DRAFT_1619 at 1639.047 kb on - strand, within EX28DRAFT_1619 at 1639.365 kb on - strand at 1639.403 kb on - strand at 1639.413 kb on - strand at 1639.419 kb on + strand at 1639.434 kb on + strand at 1639.450 kb on + strand at 1639.451 kb on - strand at 1639.544 kb on + strand at 1639.545 kb on - strand at 1639.561 kb on + strand at 1639.562 kb on - strand at 1639.562 kb on - strand at 1639.666 kb on - strand, within EX28DRAFT_1620 at 1639.700 kb on + strand at 1639.720 kb on + strand at 1639.732 kb on + strand at 1639.732 kb on + strand at 1639.733 kb on - strand at 1639.733 kb on - strand at 1639.733 kb on - strand at 1639.733 kb on - strand at 1639.735 kb on + strand at 1639.735 kb on + strand at 1639.736 kb on - strand at 1639.736 kb on - strand at 1639.736 kb on - strand at 1639.748 kb on + strand at 1639.749 kb on - strand at 1639.749 kb on - strand at 1639.907 kb on - strand at 1639.907 kb on - strand at 1639.907 kb on - strand at 1639.909 kb on + strand at 1639.920 kb on - strand at 1639.934 kb on + strand at 1639.937 kb on + strand at 1639.938 kb on - strand at 1639.938 kb on - strand at 1640.019 kb on + strand, within EX28DRAFT_1621 at 1640.020 kb on - strand, within EX28DRAFT_1621 at 1640.020 kb on - strand, within EX28DRAFT_1621 at 1640.048 kb on + strand, within EX28DRAFT_1621 at 1640.049 kb on - strand, within EX28DRAFT_1621 at 1640.051 kb on + strand, within EX28DRAFT_1621 at 1640.051 kb on + strand, within EX28DRAFT_1621 at 1640.051 kb on + strand, within EX28DRAFT_1621 at 1640.055 kb on + strand, within EX28DRAFT_1621 at 1640.127 kb on - strand, within EX28DRAFT_1621 at 1640.150 kb on - strand at 1640.151 kb on + strand at 1640.181 kb on + strand at 1640.181 kb on + strand at 1640.181 kb on + strand at 1640.181 kb on + strand at 1640.181 kb on + strand at 1640.182 kb on - strand at 1640.182 kb on - strand at 1640.183 kb on + strand at 1640.183 kb on + strand at 1640.183 kb on + strand at 1640.184 kb on - strand at 1640.184 kb on - strand at 1640.228 kb on - strand at 1640.311 kb on - strand, within EX28DRAFT_1622
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,637,340 + +0.6 1,637,383 - EX28DRAFT_1618 0.14 +0.4 1,637,522 + EX28DRAFT_1618 0.28 +3.0 1,637,522 + EX28DRAFT_1618 0.28 -0.5 1,637,623 + EX28DRAFT_1618 0.38 +0.3 1,637,624 - EX28DRAFT_1618 0.38 +0.4 1,637,659 + EX28DRAFT_1618 0.42 +0.1 1,637,660 - EX28DRAFT_1618 0.42 -0.2 1,637,669 - EX28DRAFT_1618 0.43 +0.4 1,637,986 + EX28DRAFT_1618 0.74 +0.2 1,637,987 - EX28DRAFT_1618 0.74 -0.3 1,638,272 - -0.6 1,638,433 + EX28DRAFT_1619 0.13 +0.6 1,638,434 - EX28DRAFT_1619 0.13 -0.3 1,638,434 - EX28DRAFT_1619 0.13 -0.1 1,638,434 - EX28DRAFT_1619 0.13 +0.6 1,638,518 + EX28DRAFT_1619 0.21 +2.5 1,638,524 - EX28DRAFT_1619 0.22 +0.5 1,638,621 - EX28DRAFT_1619 0.31 +0.2 1,638,706 + EX28DRAFT_1619 0.39 +0.3 1,638,706 + EX28DRAFT_1619 0.39 -1.8 1,639,047 - EX28DRAFT_1619 0.72 -1.1 1,639,365 - +1.3 1,639,403 - -0.6 1,639,413 - +0.3 1,639,419 + +0.4 1,639,434 + -0.1 1,639,450 + +0.2 1,639,451 - -0.1 1,639,544 + +0.4 1,639,545 - -0.4 1,639,561 + +1.5 1,639,562 - -1.1 1,639,562 - -1.0 1,639,666 - EX28DRAFT_1620 0.70 -1.7 1,639,700 + -0.1 1,639,720 + -0.1 1,639,732 + -0.1 1,639,732 + +0.2 1,639,733 - +0.2 1,639,733 - +0.1 1,639,733 - +1.1 1,639,733 - +1.4 1,639,735 + -0.1 1,639,735 + -0.2 1,639,736 - -0.3 1,639,736 - -0.3 1,639,736 - -0.3 1,639,748 + +0.5 1,639,749 - +0.5 1,639,749 - -0.7 1,639,907 - -1.4 1,639,907 - +0.3 1,639,907 - -0.1 1,639,909 + -0.1 1,639,920 - -0.8 1,639,934 + +0.5 1,639,937 + +0.5 1,639,938 - +0.7 1,639,938 - +0.2 1,640,019 + EX28DRAFT_1621 0.38 -0.7 1,640,020 - EX28DRAFT_1621 0.38 +0.2 1,640,020 - EX28DRAFT_1621 0.38 -1.4 1,640,048 + EX28DRAFT_1621 0.51 -1.1 1,640,049 - EX28DRAFT_1621 0.51 +0.2 1,640,051 + EX28DRAFT_1621 0.52 -0.8 1,640,051 + EX28DRAFT_1621 0.52 -0.0 1,640,051 + EX28DRAFT_1621 0.52 -0.1 1,640,055 + EX28DRAFT_1621 0.54 +0.5 1,640,127 - EX28DRAFT_1621 0.88 -1.1 1,640,150 - +0.1 1,640,151 + -1.7 1,640,181 + +0.9 1,640,181 + -0.1 1,640,181 + +0.6 1,640,181 + +0.3 1,640,181 + +2.1 1,640,182 - +0.5 1,640,182 - +0.1 1,640,183 + +0.8 1,640,183 + +1.0 1,640,183 + +1.5 1,640,184 - -0.3 1,640,184 - -1.2 1,640,228 - +0.4 1,640,311 - EX28DRAFT_1622 0.16 +1.9
Or see this region's nucleotide sequence