Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1457 and EX28DRAFT_1458 are separated by 109 nucleotides EX28DRAFT_1458 and EX28DRAFT_1459 are separated by 151 nucleotides EX28DRAFT_1459 and EX28DRAFT_1460 overlap by 1 nucleotides
EX28DRAFT_1457: EX28DRAFT_1457 - L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily, at 1,471,158 to 1,472,372
_1457
EX28DRAFT_1458: EX28DRAFT_1458 - Uncharacterized conserved protein, at 1,472,482 to 1,472,808
_1458
EX28DRAFT_1459: EX28DRAFT_1459 - Protein of unknown function (DUF1283), at 1,472,960 to 1,473,298
_1459
EX28DRAFT_1460: EX28DRAFT_1460 - Acetyltransferases, including N-acetylases of ribosomal proteins, at 1,473,298 to 1,473,858
_1460
Position (kb)
1472
1473 Strain fitness (log2 ratio)
-2
-1
0
1 at 1471.525 kb on + strand, within EX28DRAFT_1457 at 1471.526 kb on - strand, within EX28DRAFT_1457 at 1471.578 kb on + strand, within EX28DRAFT_1457 at 1471.579 kb on - strand, within EX28DRAFT_1457 at 1471.579 kb on - strand, within EX28DRAFT_1457 at 1471.608 kb on - strand, within EX28DRAFT_1457 at 1471.811 kb on - strand, within EX28DRAFT_1457 at 1472.161 kb on + strand, within EX28DRAFT_1457 at 1472.162 kb on - strand, within EX28DRAFT_1457 at 1472.162 kb on - strand, within EX28DRAFT_1457 at 1472.162 kb on - strand, within EX28DRAFT_1457 at 1472.342 kb on + strand at 1472.385 kb on + strand at 1472.386 kb on - strand at 1472.553 kb on - strand, within EX28DRAFT_1458 at 1472.609 kb on + strand, within EX28DRAFT_1458 at 1472.609 kb on + strand, within EX28DRAFT_1458 at 1472.610 kb on - strand, within EX28DRAFT_1458 at 1472.610 kb on - strand, within EX28DRAFT_1458 at 1472.663 kb on - strand, within EX28DRAFT_1458 at 1472.713 kb on + strand, within EX28DRAFT_1458 at 1472.808 kb on + strand at 1472.808 kb on + strand at 1472.835 kb on - strand at 1472.933 kb on + strand at 1472.946 kb on + strand at 1472.971 kb on - strand at 1473.318 kb on + strand at 1473.410 kb on - strand, within EX28DRAFT_1460 at 1473.565 kb on - strand, within EX28DRAFT_1460 at 1473.649 kb on + strand, within EX28DRAFT_1460 at 1473.650 kb on - strand, within EX28DRAFT_1460 at 1473.771 kb on + strand, within EX28DRAFT_1460 at 1473.771 kb on + strand, within EX28DRAFT_1460
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,471,525 + EX28DRAFT_1457 0.30 -0.1 1,471,526 - EX28DRAFT_1457 0.30 +0.0 1,471,578 + EX28DRAFT_1457 0.35 +0.3 1,471,579 - EX28DRAFT_1457 0.35 -0.4 1,471,579 - EX28DRAFT_1457 0.35 +0.7 1,471,608 - EX28DRAFT_1457 0.37 +0.1 1,471,811 - EX28DRAFT_1457 0.54 +0.8 1,472,161 + EX28DRAFT_1457 0.83 -0.2 1,472,162 - EX28DRAFT_1457 0.83 +0.2 1,472,162 - EX28DRAFT_1457 0.83 -0.4 1,472,162 - EX28DRAFT_1457 0.83 -0.5 1,472,342 + +0.2 1,472,385 + -0.0 1,472,386 - +0.1 1,472,553 - EX28DRAFT_1458 0.22 +1.3 1,472,609 + EX28DRAFT_1458 0.39 +0.4 1,472,609 + EX28DRAFT_1458 0.39 -0.1 1,472,610 - EX28DRAFT_1458 0.39 +0.2 1,472,610 - EX28DRAFT_1458 0.39 +0.6 1,472,663 - EX28DRAFT_1458 0.55 +0.5 1,472,713 + EX28DRAFT_1458 0.71 +1.4 1,472,808 + -0.3 1,472,808 + +0.2 1,472,835 - +0.4 1,472,933 + -0.3 1,472,946 + +0.3 1,472,971 - -0.2 1,473,318 + +0.0 1,473,410 - EX28DRAFT_1460 0.20 -0.8 1,473,565 - EX28DRAFT_1460 0.48 +0.3 1,473,649 + EX28DRAFT_1460 0.63 -1.8 1,473,650 - EX28DRAFT_1460 0.63 -1.4 1,473,771 + EX28DRAFT_1460 0.84 -0.5 1,473,771 + EX28DRAFT_1460 0.84 -0.7
Or see this region's nucleotide sequence