Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1414 and EX28DRAFT_1415 are separated by 2 nucleotides EX28DRAFT_1415 and EX28DRAFT_1416 overlap by 4 nucleotides EX28DRAFT_1416 and EX28DRAFT_1417 are separated by 7 nucleotides EX28DRAFT_1417 and EX28DRAFT_1418 are separated by 11 nucleotides
EX28DRAFT_1414: EX28DRAFT_1414 - phenylacetate degradation probable enoyl-CoA hydratase paaB, at 1,422,743 to 1,423,528
_1414
EX28DRAFT_1415: EX28DRAFT_1415 - Enoyl-CoA hydratase/carnithine racemase, at 1,423,531 to 1,424,298
_1415
EX28DRAFT_1416: EX28DRAFT_1416 - phenylacetate-CoA oxygenase/reductase, PaaK subunit, at 1,424,295 to 1,425,365
_1416
EX28DRAFT_1417: EX28DRAFT_1417 - phenylacetate-CoA oxygenase, PaaJ subunit, at 1,425,373 to 1,425,870
_1417
EX28DRAFT_1418: EX28DRAFT_1418 - phenylacetate-CoA oxygenase, PaaI subunit, at 1,425,882 to 1,426,628
_1418
Position (kb) 1424
1425
1426 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1423.615 kb on - strand, within EX28DRAFT_1415 at 1423.813 kb on - strand, within EX28DRAFT_1415 at 1423.896 kb on + strand, within EX28DRAFT_1415 at 1424.402 kb on - strand, within EX28DRAFT_1416 at 1424.445 kb on - strand, within EX28DRAFT_1416 at 1424.633 kb on + strand, within EX28DRAFT_1416 at 1424.863 kb on + strand, within EX28DRAFT_1416 at 1425.023 kb on - strand, within EX28DRAFT_1416 at 1425.078 kb on + strand, within EX28DRAFT_1416 at 1425.079 kb on - strand, within EX28DRAFT_1416 at 1425.196 kb on + strand, within EX28DRAFT_1416 at 1425.196 kb on + strand, within EX28DRAFT_1416 at 1425.197 kb on - strand, within EX28DRAFT_1416 at 1425.379 kb on - strand at 1425.390 kb on - strand at 1425.411 kb on + strand at 1425.411 kb on + strand at 1425.628 kb on + strand, within EX28DRAFT_1417 at 1425.704 kb on + strand, within EX28DRAFT_1417 at 1425.705 kb on - strand, within EX28DRAFT_1417 at 1425.705 kb on - strand, within EX28DRAFT_1417 at 1425.983 kb on - strand, within EX28DRAFT_1418 at 1425.987 kb on + strand, within EX28DRAFT_1418 at 1426.248 kb on - strand, within EX28DRAFT_1418
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,423,615 - EX28DRAFT_1415 0.11 +0.1 1,423,813 - EX28DRAFT_1415 0.37 +0.9 1,423,896 + EX28DRAFT_1415 0.48 +0.3 1,424,402 - EX28DRAFT_1416 0.10 +1.1 1,424,445 - EX28DRAFT_1416 0.14 +0.3 1,424,633 + EX28DRAFT_1416 0.32 +1.1 1,424,863 + EX28DRAFT_1416 0.53 +0.4 1,425,023 - EX28DRAFT_1416 0.68 -0.3 1,425,078 + EX28DRAFT_1416 0.73 -0.2 1,425,079 - EX28DRAFT_1416 0.73 +1.1 1,425,196 + EX28DRAFT_1416 0.84 -0.5 1,425,196 + EX28DRAFT_1416 0.84 +0.8 1,425,197 - EX28DRAFT_1416 0.84 -0.4 1,425,379 - -1.4 1,425,390 - -0.0 1,425,411 + -2.5 1,425,411 + +0.4 1,425,628 + EX28DRAFT_1417 0.51 +0.2 1,425,704 + EX28DRAFT_1417 0.66 -0.4 1,425,705 - EX28DRAFT_1417 0.67 -0.2 1,425,705 - EX28DRAFT_1417 0.67 -0.8 1,425,983 - EX28DRAFT_1418 0.14 -0.6 1,425,987 + EX28DRAFT_1418 0.14 +0.1 1,426,248 - EX28DRAFT_1418 0.49 +3.1
Or see this region's nucleotide sequence