Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0548 and EX28DRAFT_0549 are separated by 158 nucleotides EX28DRAFT_0549 and EX28DRAFT_0550 are separated by 46 nucleotides EX28DRAFT_0550 and EX28DRAFT_0551 are separated by 53 nucleotides EX28DRAFT_0551 and EX28DRAFT_0552 are separated by 4 nucleotides
EX28DRAFT_0548: EX28DRAFT_0548 - Predicted membrane protein, at 542,517 to 543,575
_0548
EX28DRAFT_0549: EX28DRAFT_0549 - Uncharacterized protein conserved in bacteria, at 543,734 to 544,069
_0549
EX28DRAFT_0550: EX28DRAFT_0550 - Uncharacterized conserved protein, at 544,116 to 544,451
_0550
EX28DRAFT_0551: EX28DRAFT_0551 - tRNA, at 544,505 to 544,580
_0551
EX28DRAFT_0552: EX28DRAFT_0552 - putative efflux protein, MATE family, at 544,585 to 546,196
_0552
Position (kb) 543
544
545 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 542.888 kb on + strand, within EX28DRAFT_0548 at 542.889 kb on - strand, within EX28DRAFT_0548 at 543.029 kb on + strand, within EX28DRAFT_0548 at 543.029 kb on + strand, within EX28DRAFT_0548 at 543.046 kb on - strand, within EX28DRAFT_0548 at 543.046 kb on - strand, within EX28DRAFT_0548 at 543.376 kb on - strand, within EX28DRAFT_0548 at 543.489 kb on - strand at 543.551 kb on + strand at 543.574 kb on - strand at 543.574 kb on - strand at 543.628 kb on - strand at 543.640 kb on + strand at 543.641 kb on - strand at 543.642 kb on + strand at 543.642 kb on + strand at 543.643 kb on - strand at 543.643 kb on - strand at 543.677 kb on + strand at 543.697 kb on + strand at 543.718 kb on + strand at 543.718 kb on + strand at 543.742 kb on + strand at 543.742 kb on + strand at 543.884 kb on + strand, within EX28DRAFT_0549 at 543.884 kb on + strand, within EX28DRAFT_0549 at 543.885 kb on - strand, within EX28DRAFT_0549 at 543.885 kb on - strand, within EX28DRAFT_0549 at 543.898 kb on + strand, within EX28DRAFT_0549 at 543.898 kb on + strand, within EX28DRAFT_0549 at 543.913 kb on + strand, within EX28DRAFT_0549 at 543.914 kb on - strand, within EX28DRAFT_0549 at 543.947 kb on + strand, within EX28DRAFT_0549 at 543.968 kb on + strand, within EX28DRAFT_0549 at 543.968 kb on + strand, within EX28DRAFT_0549 at 543.969 kb on - strand, within EX28DRAFT_0549 at 543.969 kb on - strand, within EX28DRAFT_0549 at 544.083 kb on + strand at 544.084 kb on - strand at 544.259 kb on + strand, within EX28DRAFT_0550 at 544.268 kb on - strand, within EX28DRAFT_0550 at 544.398 kb on - strand, within EX28DRAFT_0550 at 544.398 kb on - strand, within EX28DRAFT_0550 at 544.398 kb on - strand, within EX28DRAFT_0550 at 544.406 kb on + strand, within EX28DRAFT_0550 at 544.406 kb on + strand, within EX28DRAFT_0550 at 544.406 kb on + strand, within EX28DRAFT_0550 at 544.407 kb on - strand, within EX28DRAFT_0550 at 544.572 kb on + strand, within EX28DRAFT_0551 at 544.597 kb on - strand at 544.677 kb on + strand at 544.678 kb on - strand at 544.823 kb on + strand, within EX28DRAFT_0552 at 544.824 kb on - strand, within EX28DRAFT_0552 at 544.835 kb on - strand, within EX28DRAFT_0552 at 544.835 kb on - strand, within EX28DRAFT_0552 at 544.885 kb on + strand, within EX28DRAFT_0552 at 544.885 kb on + strand, within EX28DRAFT_0552 at 544.886 kb on - strand, within EX28DRAFT_0552 at 544.886 kb on - strand, within EX28DRAFT_0552 at 544.886 kb on - strand, within EX28DRAFT_0552 at 544.886 kb on - strand, within EX28DRAFT_0552 at 544.900 kb on + strand, within EX28DRAFT_0552 at 545.025 kb on + strand, within EX28DRAFT_0552 at 545.026 kb on - strand, within EX28DRAFT_0552 at 545.026 kb on - strand, within EX28DRAFT_0552 at 545.026 kb on - strand, within EX28DRAFT_0552
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 542,888 + EX28DRAFT_0548 0.35 -1.5 542,889 - EX28DRAFT_0548 0.35 -0.3 543,029 + EX28DRAFT_0548 0.48 +0.3 543,029 + EX28DRAFT_0548 0.48 +0.1 543,046 - EX28DRAFT_0548 0.50 -0.6 543,046 - EX28DRAFT_0548 0.50 -0.2 543,376 - EX28DRAFT_0548 0.81 +0.3 543,489 - -1.3 543,551 + -0.5 543,574 - +0.1 543,574 - +0.0 543,628 - +0.1 543,640 + -0.5 543,641 - -0.5 543,642 + -0.3 543,642 + -1.6 543,643 - +1.1 543,643 - +0.2 543,677 + -0.1 543,697 + +0.0 543,718 + -2.5 543,718 + -1.4 543,742 + -1.1 543,742 + -1.7 543,884 + EX28DRAFT_0549 0.45 -0.7 543,884 + EX28DRAFT_0549 0.45 -0.2 543,885 - EX28DRAFT_0549 0.45 -1.2 543,885 - EX28DRAFT_0549 0.45 -1.0 543,898 + EX28DRAFT_0549 0.49 -0.9 543,898 + EX28DRAFT_0549 0.49 -0.0 543,913 + EX28DRAFT_0549 0.53 -0.4 543,914 - EX28DRAFT_0549 0.54 -1.9 543,947 + EX28DRAFT_0549 0.63 -0.9 543,968 + EX28DRAFT_0549 0.70 -1.0 543,968 + EX28DRAFT_0549 0.70 -0.4 543,969 - EX28DRAFT_0549 0.70 +1.3 543,969 - EX28DRAFT_0549 0.70 -2.7 544,083 + +0.3 544,084 - +0.2 544,259 + EX28DRAFT_0550 0.43 +0.9 544,268 - EX28DRAFT_0550 0.45 -3.8 544,398 - EX28DRAFT_0550 0.84 -0.8 544,398 - EX28DRAFT_0550 0.84 +0.2 544,398 - EX28DRAFT_0550 0.84 +0.8 544,406 + EX28DRAFT_0550 0.86 +0.9 544,406 + EX28DRAFT_0550 0.86 -0.2 544,406 + EX28DRAFT_0550 0.86 -1.1 544,407 - EX28DRAFT_0550 0.87 -0.8 544,572 + EX28DRAFT_0551 0.88 +0.1 544,597 - +0.6 544,677 + +1.8 544,678 - +0.2 544,823 + EX28DRAFT_0552 0.15 +1.1 544,824 - EX28DRAFT_0552 0.15 -0.1 544,835 - EX28DRAFT_0552 0.16 -0.2 544,835 - EX28DRAFT_0552 0.16 -0.0 544,885 + EX28DRAFT_0552 0.19 -0.5 544,885 + EX28DRAFT_0552 0.19 +0.9 544,886 - EX28DRAFT_0552 0.19 +0.2 544,886 - EX28DRAFT_0552 0.19 -0.7 544,886 - EX28DRAFT_0552 0.19 +0.1 544,886 - EX28DRAFT_0552 0.19 +2.1 544,900 + EX28DRAFT_0552 0.20 +0.5 545,025 + EX28DRAFT_0552 0.27 +0.4 545,026 - EX28DRAFT_0552 0.27 -0.5 545,026 - EX28DRAFT_0552 0.27 -0.5 545,026 - EX28DRAFT_0552 0.27 +0.1
Or see this region's nucleotide sequence