Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0537 and EX28DRAFT_0538 overlap by 4 nucleotides EX28DRAFT_0538 and EX28DRAFT_0539 overlap by 4 nucleotides
EX28DRAFT_0537: EX28DRAFT_0537 - imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 530,779 to 531,846
_0537
EX28DRAFT_0538: EX28DRAFT_0538 - histidinol-phosphate aminotransferase, at 531,843 to 532,904
_0538
EX28DRAFT_0539: EX28DRAFT_0539 - histidinol dehydrogenase, at 532,901 to 534,205
_0539
Position (kb) 531
532
533 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 531.003 kb on + strand, within EX28DRAFT_0537 at 531.005 kb on + strand, within EX28DRAFT_0537 at 531.173 kb on + strand, within EX28DRAFT_0537 at 531.174 kb on - strand, within EX28DRAFT_0537 at 531.174 kb on - strand, within EX28DRAFT_0537 at 531.174 kb on - strand, within EX28DRAFT_0537 at 531.237 kb on + strand, within EX28DRAFT_0537 at 531.387 kb on + strand, within EX28DRAFT_0537 at 531.470 kb on + strand, within EX28DRAFT_0537 at 531.470 kb on + strand, within EX28DRAFT_0537 at 531.470 kb on + strand, within EX28DRAFT_0537 at 531.471 kb on - strand, within EX28DRAFT_0537 at 531.471 kb on - strand, within EX28DRAFT_0537 at 531.471 kb on - strand, within EX28DRAFT_0537 at 531.471 kb on - strand, within EX28DRAFT_0537 at 531.471 kb on - strand, within EX28DRAFT_0537 at 531.471 kb on - strand, within EX28DRAFT_0537 at 531.589 kb on + strand, within EX28DRAFT_0537 at 531.628 kb on - strand, within EX28DRAFT_0537 at 531.703 kb on - strand, within EX28DRAFT_0537 at 531.805 kb on - strand at 531.846 kb on + strand at 531.906 kb on - strand at 531.939 kb on - strand at 531.956 kb on + strand, within EX28DRAFT_0538 at 532.018 kb on + strand, within EX28DRAFT_0538 at 532.019 kb on - strand, within EX28DRAFT_0538 at 532.022 kb on + strand, within EX28DRAFT_0538 at 532.023 kb on - strand, within EX28DRAFT_0538 at 532.082 kb on - strand, within EX28DRAFT_0538 at 532.355 kb on - strand, within EX28DRAFT_0538 at 532.576 kb on + strand, within EX28DRAFT_0538 at 532.589 kb on + strand, within EX28DRAFT_0538 at 532.590 kb on - strand, within EX28DRAFT_0538 at 532.590 kb on - strand, within EX28DRAFT_0538 at 532.600 kb on + strand, within EX28DRAFT_0538 at 532.705 kb on + strand, within EX28DRAFT_0538 at 532.856 kb on - strand at 533.179 kb on + strand, within EX28DRAFT_0539 at 533.569 kb on - strand, within EX28DRAFT_0539 at 533.569 kb on - strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.661 kb on + strand, within EX28DRAFT_0539 at 533.662 kb on - strand, within EX28DRAFT_0539 at 533.662 kb on - strand, within EX28DRAFT_0539 at 533.817 kb on + strand, within EX28DRAFT_0539 at 533.818 kb on - strand, within EX28DRAFT_0539 at 533.818 kb on - strand, within EX28DRAFT_0539
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 531,003 + EX28DRAFT_0537 0.21 -2.3 531,005 + EX28DRAFT_0537 0.21 -2.9 531,173 + EX28DRAFT_0537 0.37 -2.1 531,174 - EX28DRAFT_0537 0.37 -3.8 531,174 - EX28DRAFT_0537 0.37 -3.8 531,174 - EX28DRAFT_0537 0.37 -0.9 531,237 + EX28DRAFT_0537 0.43 -3.2 531,387 + EX28DRAFT_0537 0.57 -1.2 531,470 + EX28DRAFT_0537 0.65 -3.2 531,470 + EX28DRAFT_0537 0.65 -2.5 531,470 + EX28DRAFT_0537 0.65 -4.0 531,471 - EX28DRAFT_0537 0.65 -3.2 531,471 - EX28DRAFT_0537 0.65 -3.5 531,471 - EX28DRAFT_0537 0.65 -1.7 531,471 - EX28DRAFT_0537 0.65 -2.8 531,471 - EX28DRAFT_0537 0.65 -2.0 531,471 - EX28DRAFT_0537 0.65 -3.0 531,589 + EX28DRAFT_0537 0.76 -2.2 531,628 - EX28DRAFT_0537 0.79 -4.0 531,703 - EX28DRAFT_0537 0.87 -4.8 531,805 - -3.9 531,846 + -4.0 531,906 - -4.1 531,939 - -3.2 531,956 + EX28DRAFT_0538 0.11 -1.9 532,018 + EX28DRAFT_0538 0.16 -2.8 532,019 - EX28DRAFT_0538 0.17 -2.9 532,022 + EX28DRAFT_0538 0.17 -3.2 532,023 - EX28DRAFT_0538 0.17 -3.2 532,082 - EX28DRAFT_0538 0.23 -3.0 532,355 - EX28DRAFT_0538 0.48 -2.8 532,576 + EX28DRAFT_0538 0.69 -4.3 532,589 + EX28DRAFT_0538 0.70 -0.4 532,590 - EX28DRAFT_0538 0.70 -1.7 532,590 - EX28DRAFT_0538 0.70 -3.1 532,600 + EX28DRAFT_0538 0.71 -3.0 532,705 + EX28DRAFT_0538 0.81 -2.7 532,856 - -1.9 533,179 + EX28DRAFT_0539 0.21 -1.6 533,569 - EX28DRAFT_0539 0.51 +0.0 533,569 - EX28DRAFT_0539 0.51 -2.0 533,659 + EX28DRAFT_0539 0.58 -4.6 533,659 + EX28DRAFT_0539 0.58 -3.5 533,659 + EX28DRAFT_0539 0.58 -3.0 533,660 - EX28DRAFT_0539 0.58 -2.7 533,660 - EX28DRAFT_0539 0.58 -2.7 533,660 - EX28DRAFT_0539 0.58 -2.1 533,661 + EX28DRAFT_0539 0.58 -3.6 533,662 - EX28DRAFT_0539 0.58 -2.3 533,662 - EX28DRAFT_0539 0.58 -1.6 533,817 + EX28DRAFT_0539 0.70 -3.2 533,818 - EX28DRAFT_0539 0.70 -4.0 533,818 - EX28DRAFT_0539 0.70 -1.3
Or see this region's nucleotide sequence