Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0533 and EX28DRAFT_0534 overlap by 7 nucleotides EX28DRAFT_0534 and EX28DRAFT_0535 overlap by 19 nucleotides EX28DRAFT_0535 and EX28DRAFT_0536 overlap by 1 nucleotides EX28DRAFT_0536 and EX28DRAFT_0537 overlap by 1 nucleotides
EX28DRAFT_0533: EX28DRAFT_0533 - phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 528,089 to 528,700
_0533
EX28DRAFT_0534: EX28DRAFT_0534 - imidazoleglycerol phosphate synthase, cyclase subunit, at 528,694 to 529,470
_0534
EX28DRAFT_0535: EX28DRAFT_0535 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC 5.3.1.16), at 529,452 to 530,189
_0535
EX28DRAFT_0536: EX28DRAFT_0536 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 530,189 to 530,779
_0536
EX28DRAFT_0537: EX28DRAFT_0537 - imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 530,779 to 531,846
_0537
Position (kb) 529
530
531 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 528.592 kb on + strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.593 kb on - strand, within EX28DRAFT_0533 at 528.595 kb on - strand, within EX28DRAFT_0533 at 528.731 kb on + strand at 528.750 kb on + strand at 528.751 kb on - strand at 528.751 kb on - strand at 529.053 kb on - strand, within EX28DRAFT_0534 at 529.074 kb on - strand, within EX28DRAFT_0534 at 529.098 kb on + strand, within EX28DRAFT_0534 at 529.099 kb on - strand, within EX28DRAFT_0534 at 529.177 kb on - strand, within EX28DRAFT_0534 at 529.322 kb on + strand, within EX28DRAFT_0534 at 529.323 kb on - strand, within EX28DRAFT_0534 at 529.435 kb on + strand at 529.450 kb on + strand at 529.450 kb on + strand at 529.450 kb on + strand at 529.451 kb on - strand at 529.451 kb on - strand at 529.490 kb on + strand at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.566 kb on + strand, within EX28DRAFT_0535 at 529.567 kb on - strand, within EX28DRAFT_0535 at 530.055 kb on + strand, within EX28DRAFT_0535 at 530.055 kb on + strand, within EX28DRAFT_0535 at 530.157 kb on - strand at 530.184 kb on - strand at 530.305 kb on - strand, within EX28DRAFT_0536 at 530.346 kb on + strand, within EX28DRAFT_0536 at 530.649 kb on - strand, within EX28DRAFT_0536 at 530.707 kb on - strand, within EX28DRAFT_0536 at 530.781 kb on - strand at 531.003 kb on + strand, within EX28DRAFT_0537 at 531.005 kb on + strand, within EX28DRAFT_0537 at 531.173 kb on + strand, within EX28DRAFT_0537 at 531.174 kb on - strand, within EX28DRAFT_0537 at 531.174 kb on - strand, within EX28DRAFT_0537 at 531.174 kb on - strand, within EX28DRAFT_0537
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 528,592 + EX28DRAFT_0533 0.82 -0.2 528,593 - EX28DRAFT_0533 0.82 -3.6 528,593 - EX28DRAFT_0533 0.82 -2.3 528,593 - EX28DRAFT_0533 0.82 -2.7 528,593 - EX28DRAFT_0533 0.82 -4.9 528,593 - EX28DRAFT_0533 0.82 -2.5 528,595 - EX28DRAFT_0533 0.83 -1.8 528,731 + -3.2 528,750 + -3.8 528,751 - -3.5 528,751 - -2.5 529,053 - EX28DRAFT_0534 0.46 -1.8 529,074 - EX28DRAFT_0534 0.49 -1.9 529,098 + EX28DRAFT_0534 0.52 -3.2 529,099 - EX28DRAFT_0534 0.52 -2.1 529,177 - EX28DRAFT_0534 0.62 +0.1 529,322 + EX28DRAFT_0534 0.81 -2.6 529,323 - EX28DRAFT_0534 0.81 -2.0 529,435 + -2.3 529,450 + -5.6 529,450 + -3.3 529,450 + +0.3 529,451 - -4.0 529,451 - -4.1 529,490 + -1.6 529,566 + EX28DRAFT_0535 0.15 -1.6 529,566 + EX28DRAFT_0535 0.15 -3.3 529,566 + EX28DRAFT_0535 0.15 -2.2 529,567 - EX28DRAFT_0535 0.16 -3.3 530,055 + EX28DRAFT_0535 0.82 -2.3 530,055 + EX28DRAFT_0535 0.82 -2.5 530,157 - -2.7 530,184 - -0.6 530,305 - EX28DRAFT_0536 0.20 -2.1 530,346 + EX28DRAFT_0536 0.27 -2.2 530,649 - EX28DRAFT_0536 0.78 -4.8 530,707 - EX28DRAFT_0536 0.88 -0.9 530,781 - -2.1 531,003 + EX28DRAFT_0537 0.21 -2.3 531,005 + EX28DRAFT_0537 0.21 -2.9 531,173 + EX28DRAFT_0537 0.37 -2.1 531,174 - EX28DRAFT_0537 0.37 -3.8 531,174 - EX28DRAFT_0537 0.37 -3.8 531,174 - EX28DRAFT_0537 0.37 -0.9
Or see this region's nucleotide sequence