Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0200 and EX28DRAFT_0201 are separated by 212 nucleotides EX28DRAFT_0201 and EX28DRAFT_0202 overlap by 14 nucleotides EX28DRAFT_0202 and EX28DRAFT_0203 are separated by 63 nucleotides EX28DRAFT_0203 and EX28DRAFT_0204 are separated by 92 nucleotides
EX28DRAFT_0200: EX28DRAFT_0200 - N-acetylmuramoyl-L-alanine amidase, at 155,793 to 156,668
_0200
EX28DRAFT_0201: EX28DRAFT_0201 - Acetyltransferase (GNAT) family, at 156,881 to 157,306
_0201
EX28DRAFT_0202: EX28DRAFT_0202 - Protein of unknown function (DUF2919), at 157,293 to 157,742
_0202
EX28DRAFT_0203: EX28DRAFT_0203 - Protein of unknown function (DUF1131), at 157,806 to 158,381
_0203
EX28DRAFT_0204: EX28DRAFT_0204 - Dyp-type peroxidase family, at 158,474 to 159,373
_0204
Position (kb) 157
158 Strain fitness (log2 ratio) -2
-1
0
1 at 156.319 kb on + strand, within EX28DRAFT_0200 at 156.319 kb on + strand, within EX28DRAFT_0200 at 156.680 kb on - strand at 156.703 kb on + strand at 156.704 kb on - strand at 156.705 kb on + strand at 156.706 kb on - strand at 156.711 kb on - strand at 156.768 kb on - strand at 156.878 kb on - strand at 156.988 kb on + strand, within EX28DRAFT_0201 at 156.988 kb on + strand, within EX28DRAFT_0201 at 156.988 kb on + strand, within EX28DRAFT_0201 at 156.989 kb on - strand, within EX28DRAFT_0201 at 157.088 kb on + strand, within EX28DRAFT_0201 at 157.092 kb on - strand, within EX28DRAFT_0201 at 157.092 kb on - strand, within EX28DRAFT_0201 at 157.232 kb on + strand, within EX28DRAFT_0201 at 157.232 kb on + strand, within EX28DRAFT_0201 at 157.232 kb on + strand, within EX28DRAFT_0201 at 157.233 kb on - strand, within EX28DRAFT_0201 at 157.233 kb on - strand, within EX28DRAFT_0201 at 157.233 kb on - strand, within EX28DRAFT_0201 at 157.233 kb on - strand, within EX28DRAFT_0201 at 157.406 kb on - strand, within EX28DRAFT_0202 at 157.456 kb on - strand, within EX28DRAFT_0202 at 157.787 kb on - strand at 157.878 kb on - strand, within EX28DRAFT_0203 at 158.045 kb on + strand, within EX28DRAFT_0203 at 158.046 kb on - strand, within EX28DRAFT_0203 at 158.046 kb on - strand, within EX28DRAFT_0203 at 158.056 kb on - strand, within EX28DRAFT_0203 at 158.058 kb on + strand, within EX28DRAFT_0203 at 158.165 kb on - strand, within EX28DRAFT_0203 at 158.193 kb on + strand, within EX28DRAFT_0203 at 158.194 kb on - strand, within EX28DRAFT_0203 at 158.194 kb on - strand, within EX28DRAFT_0203 at 158.195 kb on + strand, within EX28DRAFT_0203 at 158.256 kb on - strand, within EX28DRAFT_0203 at 158.283 kb on + strand, within EX28DRAFT_0203 at 158.283 kb on + strand, within EX28DRAFT_0203 at 158.284 kb on - strand, within EX28DRAFT_0203 at 158.342 kb on + strand at 158.343 kb on - strand at 158.379 kb on + strand at 158.379 kb on + strand at 158.419 kb on - strand at 158.420 kb on + strand at 158.423 kb on + strand at 158.458 kb on + strand at 158.459 kb on - strand at 158.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 156,319 + EX28DRAFT_0200 0.60 -1.0 156,319 + EX28DRAFT_0200 0.60 +0.3 156,680 - +0.0 156,703 + +0.1 156,704 - -1.3 156,705 + -1.1 156,706 - +0.3 156,711 - +0.3 156,768 - -0.3 156,878 - -0.2 156,988 + EX28DRAFT_0201 0.25 -0.8 156,988 + EX28DRAFT_0201 0.25 +1.6 156,988 + EX28DRAFT_0201 0.25 -0.9 156,989 - EX28DRAFT_0201 0.25 +0.6 157,088 + EX28DRAFT_0201 0.49 +0.1 157,092 - EX28DRAFT_0201 0.50 -1.2 157,092 - EX28DRAFT_0201 0.50 +1.1 157,232 + EX28DRAFT_0201 0.82 -0.4 157,232 + EX28DRAFT_0201 0.82 -0.3 157,232 + EX28DRAFT_0201 0.82 +0.8 157,233 - EX28DRAFT_0201 0.83 +0.5 157,233 - EX28DRAFT_0201 0.83 +0.3 157,233 - EX28DRAFT_0201 0.83 -0.2 157,233 - EX28DRAFT_0201 0.83 -0.5 157,406 - EX28DRAFT_0202 0.25 -2.1 157,456 - EX28DRAFT_0202 0.36 -0.3 157,787 - -0.4 157,878 - EX28DRAFT_0203 0.12 -0.2 158,045 + EX28DRAFT_0203 0.41 +1.1 158,046 - EX28DRAFT_0203 0.42 +0.2 158,046 - EX28DRAFT_0203 0.42 +0.0 158,056 - EX28DRAFT_0203 0.43 -1.3 158,058 + EX28DRAFT_0203 0.44 -0.3 158,165 - EX28DRAFT_0203 0.62 -0.4 158,193 + EX28DRAFT_0203 0.67 +0.2 158,194 - EX28DRAFT_0203 0.67 -0.9 158,194 - EX28DRAFT_0203 0.67 +0.6 158,195 + EX28DRAFT_0203 0.68 +0.5 158,256 - EX28DRAFT_0203 0.78 -0.2 158,283 + EX28DRAFT_0203 0.83 +0.2 158,283 + EX28DRAFT_0203 0.83 -0.3 158,284 - EX28DRAFT_0203 0.83 -0.0 158,342 + -1.3 158,343 - -0.4 158,379 + -0.2 158,379 + -0.1 158,419 - -0.1 158,420 + +0.6 158,423 + -0.1 158,458 + -0.5 158,459 - +1.6 158,543 - -0.2
Or see this region's nucleotide sequence